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Li P, Yu H, Ni ZY, et al. High Expression of a cDNA Coding for Human Lymphotoxin alpha Derivative in E. coli and Purification of Its Protein Product. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1998;30(3):288-292
Li, P., Yu, H., Ni, Z. Y., Chen, C. M., Zhao, S. Y., & Li, C. B. (1998). High Expression of a cDNA Coding for Human Lymphotoxin alpha Derivative in E. coli and Purification of Its Protein Product. Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica, 30(3), 288-292.
Li, Ping, et al. "High Expression of a cDNA Coding for Human Lymphotoxin alpha Derivative in E. coli and Purification of Its Protein Product." Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica vol. 30,3 (1998): 288-292.
Li P, Yu H, Ni ZY, Chen CM, Zhao SY, Li CB. High Expression of a cDNA Coding for Human Lymphotoxin alpha Derivative in E. coli and Purification of Its Protein Product. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1998;30(3):288-292. PMID: 12174263.
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Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1998;30(3):288-292.

High Expression of a cDNA Coding for Human Lymphotoxin alpha Derivative in E. coli and Purification of Its Protein Product.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

Ping Li, Hong Yu, Zhuo-Yu Ni, Chun-Mei Chen, Shou-Yuan Zhao, Chang-Ben Li

Affiliations

  1. Institute of Genetics, State Key Laboratory of Genetic Engineering, Fudan University, Shanghai 200433, China. [email protected]

PMID: 12174263

Abstract

Using PCR, a cDNA coding for human lymphotoxin alpha derivative (hLTalphaDa) lacking 27 amino acids at N-terminal of natural hLT was constructed. The expression construct was expressed in E. coli BL21 (DE3). The product of expression was in the form of inclusion bodies, and accounted for 60%-80% of total bacterial proteins. The product protein was purified to over 95% by treatments of inclusion bodies. The specific activity of hLTalphaDa was above 2x10(8) IU(per mg protein). Its cytotoxic activity can be neutralized by monoclone antibody against hLT. The anti-tumor effects of hLTalphaDa were also tested in vitro and in vivo.

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