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Kim HA, Whittle SC, Lee S, et al. Brain immune cell composition and functional outcome after cerebral ischemia: comparison of two mouse strains. Front Cell Neurosci. 2014;8:365doi: 10.3389/fncel.2014.00365.
Kim, H. A., Whittle, S. C., Lee, S., Chu, H. X., Zhang, S. R., Wei, Z., Arumugam, T. V., Vinh, A., Drummond, G. R., & Sobey, C. G. (2014). Brain immune cell composition and functional outcome after cerebral ischemia: comparison of two mouse strains. Frontiers in cellular neuroscience, 8365. https://doi.org/10.3389/fncel.2014.00365
Kim, Hyun Ah, et al. "Brain immune cell composition and functional outcome after cerebral ischemia: comparison of two mouse strains." Frontiers in cellular neuroscience vol. 8 (2014): 365. doi: https://doi.org/10.3389/fncel.2014.00365
Kim HA, Whittle SC, Lee S, Chu HX, Zhang SR, Wei Z, Arumugam TV, Vinh A, Drummond GR, Sobey CG. Brain immune cell composition and functional outcome after cerebral ischemia: comparison of two mouse strains. Front Cell Neurosci. 2014 Nov 19;8:365. doi: 10.3389/fncel.2014.00365. eCollection 2014. PMID: 25477780; PMCID: PMC4237143.
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Front Cell Neurosci. 2014 Nov 19;8:365. doi: 10.3389/fncel.2014.00365. eCollection 2014.

Brain immune cell composition and functional outcome after cerebral ischemia: comparison of two mouse strains.

Frontiers in cellular neuroscience

Hyun Ah Kim, Stephanie C Whittle, Seyoung Lee, Hannah X Chu, Shenpeng R Zhang, Zihui Wei, Thiruma V Arumugam, Anthony Vinh, Grant R Drummond, Christopher G Sobey

Affiliations

  1. Department of Pharmacology, Monash University Clayton, VIC, Australia.
  2. Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore Singapore, Singapore ; School of Pharmacy, Sungkyunkwan University Suwon, South Korea ; School of Biomedical Sciences, The University of Queensland St Lucia, QLD, Australia.
  3. Department of Pharmacology, Monash University Clayton, VIC, Australia ; Department of Surgery, Monash Medical Centre, Southern Clinical School, Monash University Clayton, VIC, Australia.

PMID: 25477780 PMCID: PMC4237143 DOI: 10.3389/fncel.2014.00365

Abstract

Inflammatory cells may contribute to secondary brain injury following cerebral ischemia. The C57Bl/6 mouse strain is known to exhibit a T helper 1-prone, pro-inflammatory type response to injury, whereas the FVB strain is relatively T helper 2-prone, or anti-inflammatory, in its immune response. We tested whether stroke outcome is more severe in C57Bl/6 than FVB mice. Male mice of each strain underwent sham surgery or 1 h occlusion of the middle cerebral artery followed by 23 h of reperfusion. Despite no difference in infarct size, C57Bl/6 mice displayed markedly greater functional deficits than FVB mice after stroke, as assessed by neurological scoring and hanging wire test. Total numbers of CD45(+) leukocytes tended to be larger in the brains of C57Bl/6 than FVB mice after stroke, but there were marked differences in leukocyte composition between the two mouse strains. The inflammatory response in C57Bl/6 mice primarily involved T and B lymphocytes, whereas neutrophils, monocytes and macrophages were more prominent in FVB mice. Our data are consistent with the concept that functional outcome after stroke is dependent on the immune cell composition which develops following ischemic brain injury.

Keywords: Th1/Th2 balance; cerebral ischemia-reperfusion; immune cell infiltration; inflammation; middle cerebral artery occlusion; stroke

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