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Raptis L, Whitfield JF. Protein kinase C stimulation increases the transforming ability of the polyoma virus middle T antigen. Biochem Biophys Res Commun. 1986;140(3):1106-12doi: 10.1016/0006-291x(86)90749-7.
Raptis, L., & Whitfield, J. F. (1986). Protein kinase C stimulation increases the transforming ability of the polyoma virus middle T antigen. Biochemical and biophysical research communications, 140(3), 1106-12. https://doi.org/10.1016/0006-291x(86)90749-7
Raptis, L, and Whitfield, J F. "Protein kinase C stimulation increases the transforming ability of the polyoma virus middle T antigen." Biochemical and biophysical research communications vol. 140,3 (1986): 1106-12. doi: https://doi.org/10.1016/0006-291x(86)90749-7
Raptis L, Whitfield JF. Protein kinase C stimulation increases the transforming ability of the polyoma virus middle T antigen. Biochem Biophys Res Commun. 1986 Nov 14;140(3):1106-12. doi: 10.1016/0006-291x(86)90749-7. PMID: 2430571.
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Biochem Biophys Res Commun. 1986 Nov 14;140(3):1106-12. doi: 10.1016/0006-291x(86)90749-7.

Protein kinase C stimulation increases the transforming ability of the polyoma virus middle T antigen.

Biochemical and biophysical research communications

L Raptis, J F Whitfield

PMID: 2430571 DOI: 10.1016/0006-291x(86)90749-7

Abstract

Exposure of dexamethasone-treated cells of the mT-1 line of F111 rat cells bearing a dexamethasone-inducible polyoma virus middle T (mT) antigen gene to very low concentrations of the protein kinase C-stimulating phorbol ester TPA increased the association of mT antigen with the cellular pp60c-src tyrosine protein kinase, as indicated by an increased phosphorylation of tyrosine residues of mT in mT:pp60c-src complexes precipitated from extracts of the TPA-treated cells by anti-mT antibodies. This TPA (hence probably protein kinase C)-enhanced association of mT with pp60c-src was accompanied by a large increase in the transforming ability of mT as indicated by a much enhanced ability of TPA-treated mT-1 cells producing submaximal levels of mT to proliferate while suspended in semi-solid medium and to form foci on confluent monolayers of normal F111 cells. NRCC NO: 26558.

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