Display options
Share it on

Neural Regen Res. 2015 Jun;10(6):932-7. doi: 10.4103/1673-5374.158353.

Hydrogen sulfide intervention in focal cerebral ischemia/reperfusion injury in rats.

Neural regeneration research

Xin-Juan Li, Chao-Kun Li, Lin-Yu Wei, Na Lu, Guo-Hong Wang, Hong-Gang Zhao, Dong-Liang Li

Affiliations

  1. Department of Physiology and Neurobiology, Xinxiang Medical University, Xinxiang, Henan Province, China.

PMID: 26199610 PMCID: PMC4498355 DOI: 10.4103/1673-5374.158353

Abstract

The present study aimed to explore the mechanism underlying the protective effects of hydrogen sulfide against neuronal damage caused by cerebral ischemia/reperfusion. We established the middle cerebral artery occlusion model in rats via the suture method. Ten minutes after middle cerebral artery occlusion, the animals were intraperitoneally injected with hydrogen sulfide donor compound sodium hydrosulfide. Immunofluorescence revealed that the immunoreactivity of P2X7 in the cerebral cortex and hippocampal CA1 region in rats with cerebral ischemia/reperfusion injury decreased with hydrogen sulfide treatment. Furthermore, treatment of these rats with hydrogen sulfide significantly lowered mortality, the Longa neurological deficit scores, and infarct volume. These results indicate that hydrogen sulfide may be protective in rats with local cerebral ischemia/reperfusion injury by down-regulating the expression of P2X7 receptors.

Keywords: 2,3,5-triphenyl-2H-tetrazolium chloride staining; NSFC grant; P2X7 receptor; animal model; brain injury; cerebral ischemia/reperfusion injury; hydrogen sulfide; immunofluorescence; middle cerebral artery occlusion; nerve regeneration; neural regeneration; protection; sodium hydrosulfide

References

  1. Methods Enzymol. 2015;554:169-86 - PubMed
  2. Glia. 2009 Feb;57(3):307-19 - PubMed
  3. J Neurosci Res. 2015 Mar;93(3):487-94 - PubMed
  4. Stroke. 1989 Jan;20(1):84-91 - PubMed
  5. Brain Res. 2013 Jan 23;1491:188-96 - PubMed
  6. J Cereb Blood Flow Metab. 2006 Jul;26(7):974-82 - PubMed
  7. Clin Exp Pharmacol Physiol. 2012 Sep;39(9):765-71 - PubMed
  8. Neurochem Int. 2005 Nov;47(6):442-8 - PubMed
  9. Neurobiol Learn Mem. 2013 Sep;104:82-91 - PubMed
  10. Neurochem Int. 2013 Dec;63(8):826-31 - PubMed
  11. Brain Res Bull. 2010 Oct 30;83(5):196-201 - PubMed
  12. J Pharmacol Sci. 2010;114(1):41-9 - PubMed
  13. Brain Res. 2010 Jul 23;1345:197-205 - PubMed
  14. Prog Neurobiol. 2006 Apr;78(6):327-46 - PubMed
  15. Neurosci Bull. 2013 Jun;29(3):390-8 - PubMed
  16. Pharmacol Res. 2014 Jun;84:32-44 - PubMed
  17. J Neuroinflammation. 2012 Aug 17;9:202 - PubMed
  18. Recent Pat CNS Drug Discov. 2010 Jan;5(1):35-45 - PubMed
  19. FASEB J. 2010 Feb;24(2):337-45 - PubMed
  20. Rev Neurosci. 2015;26(2):129-42 - PubMed
  21. Neurobiol Dis. 2012 Mar;45(3):954-61 - PubMed
  22. J Mol Neurosci. 2014;54(2):264-70 - PubMed
  23. Br J Pharmacol. 2011 Jul;163(5):908-11 - PubMed
  24. J Neuropathol Exp Neurol. 2004 Jul;63(7):686-99 - PubMed
  25. PLoS One. 2013;8(4):e60200 - PubMed
  26. J Cereb Blood Flow Metab. 2004 Apr;24(4):392-8 - PubMed
  27. J Vis Exp. 2011 May 08;(51):null - PubMed
  28. J Alzheimers Dis. 2011;24 Suppl 2:173-82 - PubMed
  29. Neurochem Int. 2014 Jan;64:37-47 - PubMed
  30. Neurochem Res. 2011 Oct;36(10):1840-9 - PubMed
  31. J Neurosci Res. 2014 Nov;92 (11):1520-8 - PubMed
  32. Antioxid Redox Signal. 2011 Jul 15;15(2):343-52 - PubMed
  33. Proc Natl Acad Sci U S A. 2009 Jul 28;106(30):12489-93 - PubMed

Publication Types