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Gomes KA, Almeida TC, Gesteira AS, et al. ESTs from Seeds to Assist the Selective Breeding of Jatropha curcas L. for Oil and Active Compounds. Genomics Insights. 2010;3:29-56doi: 10.4137/GEI.S4340.
Gomes, K. A., Almeida, T. C., Gesteira, A. S., Lôbo, I. P., Guimarães, A. C., de Miranda, A. B., Van Sluys, M. A., da Cruz, R. S., Cascardo, J. C., & Carels, N. (2010). ESTs from Seeds to Assist the Selective Breeding of Jatropha curcas L. for Oil and Active Compounds. Genomics insights, 329-56. https://doi.org/10.4137/GEI.S4340
Gomes, Kleber A, et al. "ESTs from Seeds to Assist the Selective Breeding of Jatropha curcas L. for Oil and Active Compounds." Genomics insights vol. 3 (2010): 29-56. doi: https://doi.org/10.4137/GEI.S4340
Gomes KA, Almeida TC, Gesteira AS, Lôbo IP, Guimarães AC, de Miranda AB, Van Sluys MA, da Cruz RS, Cascardo JC, Carels N. ESTs from Seeds to Assist the Selective Breeding of Jatropha curcas L. for Oil and Active Compounds. Genomics Insights. 2010 Oct 28;3:29-56. doi: 10.4137/GEI.S4340. eCollection 2010. PMID: 26217103; PMCID: PMC4510598.
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Genomics Insights. 2010 Oct 28;3:29-56. doi: 10.4137/GEI.S4340. eCollection 2010.

ESTs from Seeds to Assist the Selective Breeding of Jatropha curcas L. for Oil and Active Compounds.

Genomics insights

Kleber A Gomes, Tiago C Almeida, Abelmon S Gesteira, Ivon P Lôbo, Ana Carolina R Guimarães, Antonio B de Miranda, Marie-Anne Van Sluys, Rosenira S da Cruz, Júlio Cm Cascardo, Nicolas Carels

Affiliations

  1. Universidade Estadual de Santa Cruz (UESC), Centro de Biotecnologia e Genética. Laboratório de Genômica e Proteômica, Ilhéus, Bahia, Brazil. ; Universidade de São Paulo, Instituto de Biociências, Departamento de Botânica, São Paulo, SP, Brazil.
  2. Universidade Estadual de Santa Cruz (UESC), Centro de Biotecnologia e Genética. Laboratório de Genômica e Proteômica, Ilhéus, Bahia, Brazil.
  3. Universidade Estadual de Santa Cruz (UESC), Centro de Biotecnologia e Genética. Laboratório de Genômica e Proteômica, Ilhéus, Bahia, Brazil. ; Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Mandioca e Fruticultura Tropical, Cruz das Almas, Bahia, Brazil.
  4. Universidade Estadual de Santa Cruz (UESC), Grupo Bioenergia e Meio Ambiente, Ilhéus, Bahia, Brazil.
  5. Fundação Oswaldo Cruz (FIOCRUZ), Instituto Oswaldo Cruz (IOC), Laboratório de Genômica Funcional e Bioinformática, Rio de Janeiro, RJ, Brazil.
  6. Universidade de São Paulo, Instituto de Biociências, Departamento de Botânica, São Paulo, SP, Brazil.
  7. Universidade Estadual de Santa Cruz (UESC), Centro de Biotecnologia e Genética. Laboratório de Genômica e Proteômica, Ilhéus, Bahia, Brazil. ; Fundação Oswaldo Cruz (FIOCRUZ), Instituto Oswaldo Cruz (IOC), Laboratório de Genômica Funcional e Bioinformática, Rio de Janeiro, RJ, Brazil.

PMID: 26217103 PMCID: PMC4510598 DOI: 10.4137/GEI.S4340

Abstract

We report here on the characterization of a cDNA library from seeds of Jatropha curcas L. at three stages of fruit maturation before yellowing. We sequenced a total of 2200 clones and obtained a set of 931 non-redundant sequences (unigenes) after trimming and quality control, ie, 140 contigs and 791 singlets with PHRED quality ≥10. We found low levels of sequence redundancy and extensive metabolic coverage by homology comparison to GO. After comparison of 5841 non-redundant ESTs from a total of 13193 reads from GenBank with KEGG, we identified tags with nucleotide variations among J. curcas accessions for genes of fatty acid, terpene, alkaloid, quinone and hormone pathways of biosynthesis. More specifically, the expression level of four genes (palmitoyl-acyl carrier protein thioesterase, 3-ketoacyl-CoA thiolase B, lysophosphatidic acid acyltransferase and geranyl pyrophosphate synthase) measured by real-time PCR proved to be significantly different between leaves and fruits. Since the nucleotide polymorphism of these tags is associated to higher level of gene expression in fruits compared to leaves, we propose this approach to speed up the search for quantitative traits in selective breeding of J. curcas. We also discuss its potential utility for the selective breeding of economically important traits in J. curcas.

Keywords: Jatropha curcas; alkaloids; biofuel; fatty acids; genomics; terpenes

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