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Sakaguchi S, Lannuzel G, Fogliani B, et al. Development of nuclear and chloroplast microsatellite markers for the endangered conifer Callitris sulcata (Cupressaceae). Appl Plant Sci. 2015;3(8)doi: 10.3732/apps.1500045.
Sakaguchi, S., Lannuzel, G., Fogliani, B., Wulff, A. S., L'Huillier, L., Kurata, S., Ueno, S., Isagi, Y., Tsumura, Y., & Ito, M. (2015). Development of nuclear and chloroplast microsatellite markers for the endangered conifer Callitris sulcata (Cupressaceae). Applications in plant sciences, 3(8), . https://doi.org/10.3732/apps.1500045
Sakaguchi, Shota, et al. "Development of nuclear and chloroplast microsatellite markers for the endangered conifer Callitris sulcata (Cupressaceae)." Applications in plant sciences vol. 3,8 (2015). doi: https://doi.org/10.3732/apps.1500045
Sakaguchi S, Lannuzel G, Fogliani B, Wulff AS, L'Huillier L, Kurata S, Ueno S, Isagi Y, Tsumura Y, Ito M. Development of nuclear and chloroplast microsatellite markers for the endangered conifer Callitris sulcata (Cupressaceae). Appl Plant Sci. 2015 Aug 10;3(8). doi: 10.3732/apps.1500045. eCollection 2015 Aug. PMID: 26312198; PMCID: PMC4542945.
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Appl Plant Sci. 2015 Aug 10;3(8). doi: 10.3732/apps.1500045. eCollection 2015 Aug.

Development of nuclear and chloroplast microsatellite markers for the endangered conifer Callitris sulcata (Cupressaceae).

Applications in plant sciences

Shota Sakaguchi, Guillaume Lannuzel, Bruno Fogliani, Adrien S Wulff, Laurent L'Huillier, Seikan Kurata, Saneyoshi Ueno, Yuji Isagi, Yoshihiko Tsumura, Motomi Ito

Affiliations

  1. Laboratory of Plant Evolution and Biodiversity, Department of General Systems Studies, Graduate School of Arts and Sciences, University of Tokyo, Komaba 1538902, Japan.
  2. Institut Agronomique néo-Calédonien (IAC), Diversités biologique et fonctionnelle des écosystèmes terrestres, BP 73, Port Laguerre, 98890 Païta, New Caledonia.
  3. Institut Agronomique néo-Calédonien (IAC), Diversités biologique et fonctionnelle des écosystèmes terrestres, BP 73, Port Laguerre, 98890 Païta, New Caledonia ; SoREco-NC, 57 Route de l'Anse Vata, 98800 Nouméa, New Caledonia.
  4. Department of Forest Genetics, Forestry, and Forest Products Research Institute, 1 Matsunosato, Tsukuba, Ibaraki 3058687, Japan.
  5. Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University, Kyoto 6068502, Japan.
  6. Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 3058577, Japan.

PMID: 26312198 PMCID: PMC4542945 DOI: 10.3732/apps.1500045

Abstract

PREMISE OF THE STUDY: Microsatellite markers were developed for Callitris sulcata (Cupressaceae), an endangered conifer species in New Caledonia.

METHODS AND RESULTS: Using sequencing by synthesis (SBS) of an RNA-Seq library, 15 polymorphic nuclear and chloroplast microsatellite markers were developed. When evaluated with 48 individuals, these markers showed genetic variations ranging from two to 15 alleles and expected heterozygosity ranging from 0 to 0.881.

CONCLUSIONS: These markers will be useful for examining the genetic diversity and structure of remaining wild populations and improving the genetic status of ex situ populations.

Keywords: Callitris sulcata; Cupressaceae; New Caledonia; microsatellites; sequencing by synthesis

References

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  2. Mol Ecol Resour. 2008 Jan;8(1):92-4 - PubMed
  3. J Mol Biol. 1990 Oct 5;215(3):403-10 - PubMed

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