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Tanouchi Y, Pai A, Park H, et al. Long-term growth data of Escherichia coli at a single-cell level. Sci Data. 2017;4:170036doi: 10.1038/sdata.2017.36.
Tanouchi, Y., Pai, A., Park, H., Huang, S., Buchler, N. E., & You, L. (2017). Long-term growth data of Escherichia coli at a single-cell level. Scientific data, 4170036. https://doi.org/10.1038/sdata.2017.36
Tanouchi, Yu, et al. "Long-term growth data of Escherichia coli at a single-cell level." Scientific data vol. 4 (2017): 170036. doi: https://doi.org/10.1038/sdata.2017.36
Tanouchi Y, Pai A, Park H, Huang S, Buchler NE, You L. Long-term growth data of Escherichia coli at a single-cell level. Sci Data. 2017 Mar 28;4:170036. doi: 10.1038/sdata.2017.36. PMID: 28350394; PMCID: PMC5369309.
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Sci Data. 2017 Mar 28;4:170036. doi: 10.1038/sdata.2017.36.

Long-term growth data of Escherichia coli at a single-cell level.

Scientific data

Yu Tanouchi, Anand Pai, Heungwon Park, Shuqiang Huang, Nicolas E Buchler, Lingchong You

Affiliations

  1. Department of Bioengineering, Stanford University, Stanford, California 94305, USA.
  2. The Gladstone Institutes (Virology and Immunology), San Francisco, San Francisco, California 94107, USA.
  3. Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.
  4. Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA.
  5. Department of Physics, Duke University, Durham, North Carolina 27708, USA.
  6. Department of Biology, Duke University, Durham, North Carolina 27708, USA.
  7. Center for Genomic and Computational Biology, Duke University, Durham, North Carolina 27708, USA.
  8. Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina 27710, USA.

PMID: 28350394 PMCID: PMC5369309 DOI: 10.1038/sdata.2017.36

Abstract

Long-term, single-cell measurement of bacterial growth is extremely valuable information, particularly in the study of homeostatic aspects such as cell-size and growth rate control. Such measurement has recently become possible due to the development of microfluidic technology. Here we present data from single-cell measurements of Escherichia coli growth over 70 generations obtained for three different growth conditions. The data were recorded every minute, and contain time course data of cell length and fluorescent intensity of constitutively expressed yellow fluorescent protein.

References

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  4. Curr Biol. 2010 Jun 22;20(12):1099-103 - PubMed
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