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Turco F, Andreozzi P, Palumbo I, et al. Bacterial stimuli activate nitric oxide colonic mucosal production in diverticular disease. Protective effects of . United European Gastroenterol J. 2016;5(5):715-724doi: 10.1177/2050640616684398.
Turco, F., Andreozzi, P., Palumbo, I., Zito, F. P., Cargiolli, M., Fiore, W., Gennarelli, N., De Palma, G. D., Sarnelli, G., & Cuomo, R. (2017). Bacterial stimuli activate nitric oxide colonic mucosal production in diverticular disease. Protective effects of . United European gastroenterology journal, 5(5), 715-724. https://doi.org/10.1177/2050640616684398
Turco, Fabio, et al. "Bacterial stimuli activate nitric oxide colonic mucosal production in diverticular disease. Protective effects of ." United European gastroenterology journal vol. 5,5 (2017): 715-724. doi: https://doi.org/10.1177/2050640616684398
Turco F, Andreozzi P, Palumbo I, Zito FP, Cargiolli M, Fiore W, Gennarelli N, De Palma GD, Sarnelli G, Cuomo R. Bacterial stimuli activate nitric oxide colonic mucosal production in diverticular disease. Protective effects of . United European Gastroenterol J. 2017 Aug;5(5):715-724. doi: 10.1177/2050640616684398. Epub 2016 Dec 11. PMID: 28815036; PMCID: PMC5548353.
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United European Gastroenterol J. 2017 Aug;5(5):715-724. doi: 10.1177/2050640616684398. Epub 2016 Dec 11.

Bacterial stimuli activate nitric oxide colonic mucosal production in diverticular disease. Protective effects of .

United European gastroenterology journal

Fabio Turco, Paolo Andreozzi, Ilaria Palumbo, Francesco Paolo Zito, Martina Cargiolli, Walter Fiore, Nicola Gennarelli, Giovanni Domenico De Palma, Giovanni Sarnelli, Rosario Cuomo

Affiliations

  1. Department of Clinical Medicine and Surgery, Federico II University of Naples, Naples, Italy.
  2. Sofar S.p.A. Trezzano Rosa, Italy.

PMID: 28815036 PMCID: PMC5548353 DOI: 10.1177/2050640616684398

Abstract

BACKGROUND: Micro-inflammation and changes in gut microbiota may play a role in the pathogenesis of diverticular disease (DD).

OBJECTIVE: The objective of this article is to evaluate the expression of nitric oxide (NO)-related mediators and S100B in colonic mucosa of patients with DD in an ex vivo model of bacterial infection.

METHODS: Intestinal biopsies obtained from patients with diverticulosis, symptomatic uncomplicated diverticular disease (SUDD) and SUDD with previous acute diverticulitis (SUDD+AD) were stimulated with the probiotic

RESULTS: Basal iNOS expression was significantly increased in SUDD and SUDD+AD patients. Basal NO expression was significantly increased in SUDD+AD. No differences in S100B release were found. In all groups, iNOS expression was significantly increased by EIEC and reduced by LCDG. In all groups, except for SUDD+AD, EIEC significantly increased NO release, whereas no increase was observed when LCDG was added to biopsies. EIEC did not induce significant changes in S100B release.

CONCLUSIONS: Colonic mucosa of patients with DD is characterized by a different reactivity toward pathogenic stimuli. LCDG plays a role in counteracting the pro-inflammatory effects exerted by EIEC, suggesting a beneficial role of this probiotic in DD.

Keywords: Diverticular disease; enteroinvasive Escherichia coli; human colonic mucosa; nitric oxide; probiotic

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