United European Gastroenterol J. 2017 Aug;5(5):715-724. doi: 10.1177/2050640616684398. Epub 2016 Dec 11.
Bacterial stimuli activate nitric oxide colonic mucosal production in diverticular disease. Protective effects of .
United European gastroenterology journal
Fabio Turco, Paolo Andreozzi, Ilaria Palumbo, Francesco Paolo Zito, Martina Cargiolli, Walter Fiore, Nicola Gennarelli, Giovanni Domenico De Palma, Giovanni Sarnelli, Rosario Cuomo
- Department of Clinical Medicine and Surgery, Federico II University of Naples, Naples, Italy.
- Sofar S.p.A. Trezzano Rosa, Italy.
PMCID: PMC5548353 DOI: 10.1177/2050640616684398
BACKGROUND: Micro-inflammation and changes in gut microbiota may play a role in the pathogenesis of diverticular disease (DD).
OBJECTIVE: The objective of this article is to evaluate the expression of nitric oxide (NO)-related mediators and S100B in colonic mucosa of patients with DD in an ex vivo model of bacterial infection.
METHODS: Intestinal biopsies obtained from patients with diverticulosis, symptomatic uncomplicated diverticular disease (SUDD) and SUDD with previous acute diverticulitis (SUDD+AD) were stimulated with the probiotic
RESULTS: Basal iNOS expression was significantly increased in SUDD and SUDD+AD patients. Basal NO expression was significantly increased in SUDD+AD. No differences in S100B release were found. In all groups, iNOS expression was significantly increased by EIEC and reduced by LCDG. In all groups, except for SUDD+AD, EIEC significantly increased NO release, whereas no increase was observed when LCDG was added to biopsies. EIEC did not induce significant changes in S100B release.
CONCLUSIONS: Colonic mucosa of patients with DD is characterized by a different reactivity toward pathogenic stimuli. LCDG plays a role in counteracting the pro-inflammatory effects exerted by EIEC, suggesting a beneficial role of this probiotic in DD.
Keywords: Diverticular disease; enteroinvasive Escherichia coli; human colonic mucosa; nitric oxide; probiotic
- Neurogastroenterol Motil. 2014 Oct;26(10 ):1458-68 - PubMed
- Mol Med. 2000 May;6(5):347-73 - PubMed
- Neurogastroenterol Motil. 2009 Nov;21(11):1209-e112 - PubMed
- Expert Opin Pharmacother. 2007 Feb;8(3):299-307 - PubMed
- J Clin Gastroenterol. 2008 Jul;42(6):699-703 - PubMed
- Clin Microbiol Rev. 1998 Jan;11(1):142-201 - PubMed