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SITS Derivatization of Peptides to Enhance 266 nm Ultraviolet Photodissociation (UVPD).

Journal of the American Society for Mass Spectrometry

Quick MM, Mehaffey MR, Johns RW, Parker WR, Brodbelt JS.
PMID: 28315237
J Am Soc Mass Spectrom. 2017 Jul;28(7):1462-1472. doi: 10.1007/s13361-017-1650-y. Epub 2017 Mar 17.

N-terminal derivatization of peptides with the chromogenic reagent 4-acetamido-4-isothiocyanatostilbene-2,2-disulfonic acid (SITS) is demonstrated to enhance the efficiency of 266 nm ultraviolet photodissociation (UVPD). Attachment of the chromophore results in a mass shift of 454 Da and provides significant gains...

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