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One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products

k-12

[No authors listed]
GSID: r0zbMngCaDgJ
KA Datsenko, BL Wanner - Proceedings of the National …, 2000 - National Acad Sciences

We have developed a simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s). In this procedure, recombination requires the phage λ Red recombinase, which is...

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Datsenko, K. A., & Wanner, B. L. (2000). One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proceedings of the National Academy of Sciences, 97(12), 6640-6645.
Datsenko, Kirill A., and Barry L. Wanner. "One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products." Proceedings of the National Academy of Sciences 97.12 (2000): 6640-6645.
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Gene in the major cotransduction gap of the Escherichia coli K-12 linkage map required for the expression of chromosomal resistance to tetracycline and other …

k-12

[No authors listed]
GSID: XkSYGmd6_usJ
AM George, SB Levy - Journal of bacteriology, 1983 - Am Soc Microbiol

… coli K-12 linkage map. We have designated the locus marA (multiple antibiotic resistance). … coli K-12 chromosome, allowed identification of the marA locus (for multiple antibiotic … Insertion of transposons through the major cotransduction gap of Escherichia coli...

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George, A. M., & Levy, S. B. (1983). Gene in the major cotransduction gap of the Escherichia coli K-12 linkage map required for the expression of chromosomal resistance to tetracycline and other antibiotics. Journal of bacteriology, 155(2), 541-548.
George, A. M., and S. B. Levy. "Gene in the major cotransduction gap of the Escherichia coli K-12 linkage map required for the expression of chromosomal resistance to tetracycline and other antibiotics." Journal of bacteriology 155.2 (1983): 541-548.
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The Escherichia coli K‐12 sheA gene encodes a 34‐kDa secreted haemolysin

k-12

[No authors listed]
GSID: 1TdT2W21jOgJ
FJ Del Castillo, SC Leal, F Moreno… - Molecular …, 1997 - Wiley Online Library

… The non-pathogenic Escherichia coli K-12 laboratory strain was considered for years to be non… coli K-12 gene, sheA, whose overexpression results in a haemolytic phenotype. It maps to … coli K-12 chromosomal haemolysin. The SheA haemolysin does not...

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Del Castillo, F. J., Leal, S. C., Moreno, F., & Castillo, I. D. (1997). The Escherichia coli K‐12 sheA gene encodes a 34‐kDa secreted haemolysin. Molecular microbiology, 25(1), 107-115.
Del Castillo, Francisco J., et al. "The Escherichia coli K‐12 sheA gene encodes a 34‐kDa secreted haemolysin." Molecular microbiology 25.1 (1997): 107-115.
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Alterations in the pathogenicity of Escherichia coli K-12 after transfer of plasmid and chromosomal genes from Shigella flexneri

k-12

[No authors listed]
GSID: qm6ubY-sGksJ
PJ Sansonetti, TL Hale, GJ Dammin… - Infection and …, 1983 - Am Soc Microbiol

… coli K-12 has facilitated the study of conjugally transferred regions of the shigella chromosome as expressed in a K-12 recipient which harbors the … coli K-12 background and assayed ina variety of laboratory models. These studies indicatethat the...

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Sansonetti, P. J., Hale, T. L., Dammin, G. J., Kapfer, C., Collins Jr, H. H., & Formal, S. B. (1983). Alterations in the pathogenicity of Escherichia coli K-12 after transfer of plasmid and chromosomal genes from Shigella flexneri. Infection and Immunity, 39(3), 1392-1402.
Sansonetti, Philippe J., et al. "Alterations in the pathogenicity of Escherichia coli K-12 after transfer of plasmid and chromosomal genes from Shigella flexneri." Infection and Immunity 39.3 (1983): 1392-1402.
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Escherichia coli K-12 Mutants Hyperproducing Chromosomal Beta-Lactamase by Gene Repetitions

k-12

[No authors listed]
GSID: MeZWtL8iYE0J
S Normark, T Edlund, T Grundström… - Journal of …, 1977 - Am Soc Microbiol

… Escherichia coli K-12 ampicillin-resistant mutants hyperproducing chromosomal /3-lactamase … Ampicillin-resistant mutants of E.coli K-12 can be isolated at a low incidence. The first … coli K-12 strains used are listed in Table 1. recA derivatives of various strains...

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Normark, S., Edlund, T., Grundström, T., Bergström, S., & Wolf-Watz, H. (1977). Escherichia coli K-12 mutants hyperproducing chromosomal beta-lactamase by gene repetitions. Journal of Bacteriology, 132(3), 912-922.
Normark, Staffan, et al. "Escherichia coli K-12 mutants hyperproducing chromosomal beta-lactamase by gene repetitions." Journal of Bacteriology 132.3 (1977): 912-922.
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Impact of a mild scrotal heating on sperm chromosomal abnormality, acrosin activity and seminal alpha‐glucosidase in human fertile males

STEM and males

Qiu Y.
GSID: R8FTBFnv8wwJ
MH Zhang, LP Zhai, ZY Fang, AN Li, Y Qiu, YX Liu - Andrologia, 2018 - Wiley Online Library

The aim of this study was to observe sperm aneuploidy, DNA integrity, seminal alpha‐glucosidase ( NAG ) and acrosin activity ( AA ) under testicular heat stress ( SH ). Spermatozoa …

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Zhang, M. H., Zhai, L. P., Fang, Z. Y., Li, A. N., Qiu, Y., & Liu, Y. X. (2018). Impact of a mild scrotal heating on sperm chromosomal abnormality, acrosin activity and seminal alpha‐glucosidase in human fertile males. Andrologia, 50(4), e12985.
Zhang, M‐H., et al. "Impact of a mild scrotal heating on sperm chromosomal abnormality, acrosin activity and seminal alpha‐glucosidase in human fertile males." Andrologia 50.4 (2018): e12985.
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A 40 kb chromosomal fragment encoding Salmonella typhimurium invasion genes is absent from the corresponding region of the Escherichia coli K‐12 chromosome

k-12

[No authors listed]
GSID: LZygYEQ8Q8cJ
DM Mills, V Bajaj, CA Lee - Molecular microbiology, 1995 - Wiley Online Library

… viously shown that some of the invasion genes from this region are not present in Escherichia coli K-12. We examined whether other S. typhimurium … coli K-12 chromosome and may represent a 'pathogenicity island'. We speculate that acquisition...

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Mills, D. M., Bajaj, V., & Lee, C. A. (1995). A 40 kb chromosomal fragment encoding Salmonella typhimurium invasion genes is absent from the corresponding region of the Escherichia coli K‐12 chromosome. Molecular microbiology, 15(4), 749-759.
Mills, Debra M., Vivek Bajaj, and Catherine A. Lee. "A 40 kb chromosomal fragment encoding Salmonella typhimurium invasion genes is absent from the corresponding region of the Escherichia coli K‐12 chromosome." Molecular microbiology 15.4 (1995): 749-759.
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At what age should we attempt to retrieve sperm from males with Klinefelter syndrome

STEM and males

[No authors listed]
GSID: fXiZwUzooFIJ
S Shepherd, R Oates - Translational Andrology and Urology, 2021 - ncbi.nlm.nih.gov

Klinefelter syndrome (KS) is a common disorder and almost every clinician in almost every sub-specialty of medicine will knowingly or unwittingly treat boys or men with a 47, XXY chromosomal constitution. Although there are numerous aspects of KS worthy...

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Shepherd, S., & Oates, R. (2021). At what age should we attempt to retrieve sperm from males with Klinefelter syndrome. Translational Andrology and Urology, 10(3), 1432.
Shepherd, Shanta, and Robert Oates. "At what age should we attempt to retrieve sperm from males with Klinefelter syndrome." Translational Andrology and Urology 10.3 (2021): 1432.
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Level of functioning of siblings and parents of probands of varying degrees of retardation.

Behavior genetics

Johnson CA, Ahern FM, Johnson RC.
PMID: 988824
Behav Genet. 1976 Oct;6(4):473-7. doi: 10.1007/BF01065704.

A further analysis of already published data supports the position that retardates of low ability level less frequently have retarded siblings, retarded parents, and parents low in occupational level than do retardates higher in ability level. The analysis supports...

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Johnson CA, Ahern FM, Johnson RC. Level of functioning of siblings and parents of probands of varying degrees of retardation. Behav Genet. 1976;6(4):473-7doi: 10.1007/BF01065704.
Johnson, C. A., Ahern, F. M., & Johnson, R. C. (1976). Level of functioning of siblings and parents of probands of varying degrees of retardation. Behavior genetics, 6(4), 473-7. https://doi.org/10.1007/BF01065704
Johnson, C A, et al. "Level of functioning of siblings and parents of probands of varying degrees of retardation." Behavior genetics vol. 6,4 (1976): 473-7. doi: https://doi.org/10.1007/BF01065704
Johnson CA, Ahern FM, Johnson RC. Level of functioning of siblings and parents of probands of varying degrees of retardation. Behav Genet. 1976 Oct;6(4):473-7. doi: 10.1007/BF01065704. PMID: 988824.
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Congenital heart disease in Down syndrome: an echocardiographic study.

Indian pediatrics

Bhatia S, Verma IC, Shrivastava S.
PMID: 1452307
Indian Pediatr. 1992 Sep;29(9):1113-6.

We evaluated the utility of echocardiography in assessing the frequency and nature of cardiac malformations in children with Down syndrome. Fifty cases of chromosomally proven Down syndrome were studied. A physical examination, electro cardiogram, radiograph of chest and two-dimensional...

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Bhatia S, Verma IC, Shrivastava S. Congenital heart disease in Down syndrome: an echocardiographic study. Indian Pediatr. 1992;29(9):1113-6
Bhatia, S., Verma, I. C., & Shrivastava, S. (1992). Congenital heart disease in Down syndrome: an echocardiographic study. Indian pediatrics, 29(9), 1113-6.
Bhatia, S, et al. "Congenital heart disease in Down syndrome: an echocardiographic study." Indian pediatrics vol. 29,9 (1992): 1113-6.
Bhatia S, Verma IC, Shrivastava S. Congenital heart disease in Down syndrome: an echocardiographic study. Indian Pediatr. 1992 Sep;29(9):1113-6. PMID: 1452307.
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Chromosoma Prize 1992.

Chromosoma

[No authors listed]
PMID: 1424990
Chromosoma. 1992 Jun;101(8):A5. doi: 10.1007/BF00352467.

No abstract available.

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Chromosoma Prize 1992. Chromosoma. 1992;101(8):A5doi: 10.1007/BF00352467.
(1992). Chromosoma Prize 1992. Chromosoma, 101(8), A5. https://doi.org/10.1007/BF00352467
"Chromosoma Prize 1992." Chromosoma vol. 101,8 (1992): A5. doi: https://doi.org/10.1007/BF00352467
Chromosoma Prize 1992. Chromosoma. 1992 Jun;101(8):A5. doi: 10.1007/BF00352467. PMID: 1424990.
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Multiplex PCR based screening for microdeletions in azoospermia factor region of Y chromosome in azoospermic and severe oligozoospermic south Indian men.

Iranian journal of reproductive medicine

Suganthi R, Vijesh V, Jayachandran S, Fathima Benazir JA.
PMID: 24639749
Iran J Reprod Med. 2013 Mar;11(3):219-26.

BACKGROUND: Y chromosomal microdeletion is an important genetic disorder, which may arise due to intrachromosomal recombination between homologous sequences in the male specific region of the human Y chromosome. It is frequently associated with the quantitative reduction of sperm....

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Suganthi R, Vijesh V, Jayachandran S, et al. Multiplex PCR based screening for microdeletions in azoospermia factor region of Y chromosome in azoospermic and severe oligozoospermic south Indian men. Iran J Reprod Med. 2013;11(3):219-26
Suganthi, R., Vijesh, V., Jayachandran, S., & Fathima Benazir, J. A. (2013). Multiplex PCR based screening for microdeletions in azoospermia factor region of Y chromosome in azoospermic and severe oligozoospermic south Indian men. Iranian journal of reproductive medicine, 11(3), 219-26.
Suganthi, Ramaswamy, et al. "Multiplex PCR based screening for microdeletions in azoospermia factor region of Y chromosome in azoospermic and severe oligozoospermic south Indian men." Iranian journal of reproductive medicine vol. 11,3 (2013): 219-26.
Suganthi R, Vijesh V, Jayachandran S, Fathima Benazir JA. Multiplex PCR based screening for microdeletions in azoospermia factor region of Y chromosome in azoospermic and severe oligozoospermic south Indian men. Iran J Reprod Med. 2013 Mar;11(3):219-26. PMID: 24639749; PMCID: PMC3943221.
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Showing 1 to 12 of 9948 entries