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Showing 1 to 12 of 19 entries
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Toward an understanding of the fluorescence intensity changes observed on fluorescein 5'-Isothiocyanate-Na(+),K (+)-ATPase.

Journal of fluorescence

Grell E, Lewitzki E, Ruf H, Brand K, Schneider FW, von der Haar T, Zachariasse KA.
PMID: 24233456
J Fluoresc. 1994 Sep;4(3):251-4. doi: 10.1007/BF01878459.

The fluorescence emission intensity between the Na(+), and the K(+) complex of Na(+),K(+)-ATPase, labeled with fluorescein 5'-isothiocyanate (FITC), differs by 30 to 40%. Experimental studies are carried out to elucidate the physical reasons which account this intensity difference. The...

High-speed free-flow electrophoresis on chip.

Analytical chemistry

Zhang CX, Manz A.
PMID: 14588015
Anal Chem. 2003 Nov 01;75(21):5759-66. doi: 10.1021/ac0345190.

A microfluidic device has been developed for continuous separation in free-flow electrophoresis (FFE) mode. A mixture of two fluorescent reagents is separated into two component streams in 75 ms using a sample flow rate of 2 nL/s. The residence...

Serological Methods for Detection of Polymyxa graminis, an Obligate Root Parasite and Vector of Plant Viruses.

Phytopathology

Delfosse P, Reddy AS, Legréve A, Devi KT, Abdurahman MD, Maraite H, Reddy DV.
PMID: 18944561
Phytopathology. 2000 May;90(5):537-45. doi: 10.1094/PHYTO.2000.90.5.537.

A purification procedure was developed to separate Polymyxa graminisresting spores from sorghum root materials. The spores were used as im-munogen to produce a polyclonal antiserum. In a direct antigen coating enzyme-linked immunosorbent assay (DAC ELISA), the antiserum could detect...

Targeted ultrasound imaging of apoptosis with annexin a5 microbubbles in acute Doxorubicin-induced cardiotoxicity.

Journal of cardiovascular ultrasound

Min PK, Lim S, Kang SJ, Hong SY, Hwang KC, Chung KH, Shim CY, Rim SJ, Chung N.
PMID: 20967156
J Cardiovasc Ultrasound. 2010 Sep;18(3):91-7. doi: 10.4250/jcu.2010.18.3.91. Epub 2010 Sep 30.

BACKGROUND: The aim of this study was to assess the feasibility of targeted ultrasound imaging on apoptosis with annexin A5 microbubbles (A5MB) in acute doxorubicin-induced cardiotoxicity.METHODS: Avidinated and octafluoropropan-filled phospholipid microbubbles were conjugated with biotinylated annexin A5. To confirm...

Uptake of biodegradable poly(γ-glutamic acid) nanoparticles and antigen presentation by dendritic cells in vivo.

Results in immunology

Uto T, Toyama M, Nishi Y, Akagi T, Shima F, Akashi M, Baba M.
PMID: 24600553
Results Immunol. 2012 Dec 03;3:1-9. doi: 10.1016/j.rinim.2012.11.002. eCollection 2013.

Poly(γ-glutamic acid) (γ-PGA) nanoparticles (NPs) carrying antigens have been shown to induce potent antigen-specific immune responses. However, in vivo delivery of γ-PGA NPs to dendritic cells (DCs), a key regulator of immune responses, still remains unclear. In this study,...

Imiquimod induced vitiligo-like lesions-A consequence of modified melanocyte function.

Immunity, inflammation and disease

Yu H, Cen J, Lin X, Cheng H, Seifert O.
PMID: 34614305
Immun Inflamm Dis. 2021 Oct 06; doi: 10.1002/iid3.543. Epub 2021 Oct 06.

INTRODUCTION: Imiquimod plays an important role in the management of condyloma and premalignant lesions. Successively, an increase of hypopigmented lesions following imiquimod application has been reported. However, the mechanisms of imiquimod on melanocytes remain unclear. This study was designed...

Sol-gel modified poly(dimethylsiloxane) microfluidic devices with high electroosmotic mobilities and hydrophilic channel wall characteristics.

Analytical chemistry

Roman GT, Hlaus T, Bass KJ, Seelhammer TG, Culbertson CT.
PMID: 15732926
Anal Chem. 2005 Mar 01;77(5):1414-22. doi: 10.1021/ac048811z.

Using a sol-gel method, we have fabricated poly(dimethylsiloxane) (PDMS) microchips with SiO2 particles homogeneously distributed within the PDMS polymer matrix. These particles are approximately 10 nm in diameter. To fabricate such devices, PDMS (Sylgard 184) was cast against SU-8...

[Microchip free flow isoelectric focusing with immobilized pH gradient on monolithic materials].

Se pu = Chinese journal of chromatography

Han B, Wang P, Zhang L, Qu F, Liang Z, Deng Y, Zhang Y.
PMID: 19938489
Se Pu. 2009 Jul;27(4):383-6.

Microchip free flow electrophoresis (microFFE) is a significant microscale technique for the continuous pre-fractionation and the preparation of valuable biological samples. In our recent work, monolithic polyacylamide (PAM) materials were polymerized in microchamber by ultraviolet (UV) initiated polymerization. With...

Oridonin induces oxidative stress-mediated cancer cells apoptosis via targeting thioredoxin reductase.

Current pharmaceutical biotechnology

Duan D, Feng X, Pan D, Wang L, Wang Y, Wang X.
PMID: 34923938
Curr Pharm Biotechnol. 2021 Dec 17; doi: 10.2174/1389201023666211217151955. Epub 2021 Dec 17.

BACKGROUND: Thioredoxin reductase (TrxR) plays vital role in regulating cellular redox balance as well as redox-mediated signal transduction. Accumulating evidence supports that overactivation of TrxR is closely related to tumorigenesis and that targeting TrxR ablation reverses the growth of...

Imiquimod induced vitiligo-like lesions-A consequence of modified melanocyte function.

Immunity, inflammation and disease

Yu H, Cen J, Lin X, Cheng H, Seifert O.
PMID: 34614305
Immun Inflamm Dis. 2022 Jan;10(1):70-77. doi: 10.1002/iid3.543. Epub 2021 Oct 06.

INTRODUCTION: Imiquimod plays an important role in the management of condyloma and premalignant lesions. Successively, an increase of hypopigmented lesions following imiquimod application has been reported. However, the mechanisms of imiquimod on melanocytes remain unclear. This study was designed...

Structure and Extent of Adsorbed Gelatin on Acrylic Latex and Polystyrene Colloidal Particles.

Journal of colloid and interface science

Vaynberg KA, Wagner NJ, Sharma R, Martic P.
PMID: 9710506
J Colloid Interface Sci. 1998 Sep 01;205(1):131-40. doi: 10.1006/jcis.1998.5640.

The extent and structure of the adsorbed layer of gelatin onto colloidal particles is measured using dynamic light scattering, small-angle neutron scattering, and dilution viscometry. Adsorption isotherms are measured using solution depletion of fluorescein-5-isothiocyanate tagged gelatin. The results show...

Imiquimod induced vitiligo-like lesions-A consequence of modified melanocyte function.

Immunity, inflammation and disease

Yu H, Cen J, Lin X, Cheng H, Seifert O.
PMID: 34614305
Immun Inflamm Dis. 2022 Jan;10(1):70-77. doi: 10.1002/iid3.543. Epub 2021 Oct 06.

INTRODUCTION: Imiquimod plays an important role in the management of condyloma and premalignant lesions. Successively, an increase of hypopigmented lesions following imiquimod application has been reported. However, the mechanisms of imiquimod on melanocytes remain unclear. This study was designed...

Showing 1 to 12 of 19 entries