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Showing 1 to 12 of 23 entries
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Chromosomal proteins in Saccharomyces cerevisiae : I. number and properties of individual proteins.

Current genetics

Gulløv K, Friis J.
PMID: 24189725
Curr Genet. 1980 Jul;2(1):69-74. doi: 10.1007/BF00445696.

Proteins were isolated from purified yeast chromatin and subjected to two-dimensional electrophoresis. The cellular and the chromosomal content of the major nonhistone proteins was measured. Two polypeptides of molecular weights 55,000 and 53,000, identified as α and β tubulin,...

Effect of amygdalin on proliferation of rat hepatic fat-storing cells and collagen production in vitro.

World journal of gastroenterology

Xu LM, Liu C, Liu P.
PMID: 27041958
World J Gastroenterol. 1997 Jun 15;3(2):103. doi: 10.3748/wjg.v3.i2.103.

AIM: To study the anti-fibrotic mechanism of amygdalin, a component of Semen Persicae, on fat-storing cells (FSC).METHODS: Livers of normal adult rats were perfused with Pronas E and collagenase in situ. FSC were isolated by centrifugation with 11% Metrizamide....

In vitro binding of riboflavin to subcellular particles from maize coleoptiles and Cucurbita hypocotyls.

Planta

Hertel R, Jesaitis AJ, Dohrmann U, Briggs WR.
PMID: 24311081
Planta. 1980 Jan;147(4):312-9. doi: 10.1007/BF00379839.

Saturable and reversible in vitro binding of [(14)C]riboflavin was found to occur on subcellular, sedimentable particles from maize coleoptiles and Cucurbita hypocotyls. The KD was ca. 6 μM, the pH optimum was near 6.0, and the number of binding...

Isolation of dendritic cells from mouse lymph nodes.

Methods in molecular medicine

Gabrilovich D.
PMID: 21374244
Methods Mol Med. 2001;64:3-7. doi: 10.1385/1-59259-150-7:3.

Lymph nodes are the primary sites of T-cell stimulation by dendritic cells (DC). After contact with antigens, DCs migrate to draining lymph nodes from the skin and other tissues (1-3). Investigation of the morphology and function of lymph node...

Circular adenovirus DNA-protein complexes from infected HeLa cell nuclei.

Virology

Girard M, Bouché JP, Marty L, Revet B, Berthelot N.
PMID: 18625474
Virology. 1977 Nov;83(1):34-55. doi: 10.1016/0042-6822(77)90209-4.

Adenovirus (Ad) DNA was extracted by the use of Sarkosyl from Ad5-infected HeLa cell nuclei at 15 hr after infection. Mature and replicating viral DNA molecules in the Sarkosyl extract were purified by sucrose gradient centrifugation followed by equilibrium...

Enriched populations of rat retinal ganglion cells: Studies using a cell-type specific surface marker.

Neurochemistry international

Beale R, Beaton DW, Neuhoff V, Osborne NN.
PMID: 20487999
Neurochem Int. 1983;5(6):691-6. doi: 10.1016/0197-0186(83)90094-3.

Cells dissociated from adult and neonatal rat retinas were separated by density gradient centrifugation. Previous work had shown that rat retinal cells labelled by an immunofluorescence assay for the Thy-1 antigen were chiefly or exclusively ganglion cells, and so...

An improved isolation procedure for yeast two-micrometer minichromosomes.

Current genetics

Shalitin C, Vishlizky A.
PMID: 24173517
Curr Genet. 1984 Dec;9(1):107-11. doi: 10.1007/BF00396211.

Two micrometer minichromosomes from Saccharomyces cerevisiae were isolated without detergent using metrizamide gradients. 2 μm minichromosomes showed a lower density in metrizamide gradients relative to genomic chromatin. Our results suggest a lower ratio of proteins to DNA in 2-μm...

Isolation of Vacuoles from Root Storage Tissue of Beta vulgaris L.

Plant physiology

Leigh RA, Branton D.
PMID: 16659738
Plant Physiol. 1976 Nov;58(5):656-62. doi: 10.1104/pp.58.5.656.

Morphologically intact and osmotically active vacuoles were isolated from root storage tissue of the red beet Beta vulgaris L., and the factors influencing both yield and stability of the vacuoles were determined. Successful isolation depended upon slicing the tissue...

Cerebrospinal fluid response following metrizamide myelography in normal dogs: effects of routine myelography and postmyelographic removal of contrast medium.

Veterinary clinical pathology

Widmer WR, Blevins WE, Cantwell HD, Cook JR, DeNicola DB.
PMID: 12684951
Vet Clin Pathol. 1990;19(3):66-76. doi: 10.1111/j.1939-165x.1990.tb00546.x.

The effect of metrizamide myelography on 90-minute postmyelographic cerebrospinal fluid (CSF) samples was evaluated in a paired crossover study in 16 normal dogs. Each dog received a routine cervical myelogram (nonwithdrawal myelography) and a myelogram followed by contrast medium...

Cytoplasmic distribution of heat shock proteins in soybean.

Plant physiology

Mansfield MA, Key JL.
PMID: 16666061
Plant Physiol. 1988 Apr;86(4):1240-6. doi: 10.1104/pp.86.4.1240.

Previous analyses of the distribution of heat shock (hs) proteins in soybean (Glycine max L. Merr., var Wayne) have demonstrated that a fraction of the low molecular weight hs protein associates with ribosomes during hs. To more specifically characterize...

Isolation of mesophyll protoplasts from mature leaves of soybeans.

Plant physiology

Lin W.
PMID: 16663331
Plant Physiol. 1983 Dec;73(4):1067-9. doi: 10.1104/pp.73.4.1067.

A procedure based on a combined cellulase-Pectolyase Y-23 enzyme digestion and metrizamide-sorbitol gradient purification protocol was developed for isolating mesophyll protoplasts from mature leaves of soybean (Glycine max L. Merr.). Based on chlorophyll content, this procedure results in a...

Cervical intervertebral disc protrusion in two horses.

The Canadian veterinary journal = La revue veterinaire canadienne

Foss RR, Genetzky RM, Riedesel EA, Graham C.
PMID: 17422269
Can Vet J. 1983 Jun;24(6):188-91.

Two horses with ataxia of all four limbs were found to have cervical intervertebral disc protrusion. Severe pelvic limb ataxia, proprioceptive deficits and spasticity were present in both horses with similar but less severe signs in the thoracic limbs....

Showing 1 to 12 of 23 entries