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Showing 1 to 12 of 153 entries
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Calibration methods and avoidance of errors in measurement of intracellular pH (pHcyt) using the indicator bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) in human platelets.

Journal of fluorescence

Valant PA, Haynes DH.
PMID: 24241630
J Fluoresc. 1992 Sep;2(3):191-206. doi: 10.1007/BF00866934.

Determinations of pHcyt in suspensions of human platelets using BCECF [bis(carboxyethyl)-5(6)-carboxyfluorescein] can be seriously biased by leakage of the fluorescent indicator. Two methods ("pH jump" and "Mn(2+)") are presented for determining the fraction of external indicator (B ext) and...

An estimation of the proton conductance of the inner membrane of turnip (Brassica napus L.) mitochondria.

Planta

Moore AL, Wilson SB.
PMID: 24414876
Planta. 1978 Jan;141(3):297-302. doi: 10.1007/BF00388347.

The permeability of the inner membrane of turnip mitochondria to H(+) and OH(-) ions has been investigated using an acid pulse technique. The rate of decay of a H(+) pulse across the inner membrane is exponential having first-order kinetics...

An Investigation of the Sustained Component of Nonphotochemical Quenching of Chlorophyll Fluorescence in Isolated Chloroplasts and Leaves of Spinach.

Plant physiology

Ruban AV, Horton P.
PMID: 12228504
Plant Physiol. 1995 Jun;108(2):721-726. doi: 10.1104/pp.108.2.721.

The slowly reversible component of nonphotochemical quenching of Chl fluorescence, ql, has been investigated in intact leaves and chloroplasts of spinach (Spinacia oleracea). In leaves, between 50 and 100% of ql (defined as the quenching that remained after at...

Solubilization and reconstitution of ca pump from corn leaf plasma membrane.

Plant physiology

Kasai M, Muto S.
PMID: 16668222
Plant Physiol. 1991 Jun;96(2):565-70. doi: 10.1104/pp.96.2.565.

The Ca(2+) transport system of corn (Zea mays) leaf plasma membrane is composed of Ca(2+) pump and Ca(2+)/H(+) antiporter driven by H(+) gradient imposed by a H(+) pump (M Kasai, S Muto [1990] J Membr Biol 114: 133-142). It...

Anion-sensitive, h-pumping ATPase in membrane vesicles from oat roots.

Plant physiology

Churchill KA, Sze H.
PMID: 16662875
Plant Physiol. 1983 Mar;71(3):610-7. doi: 10.1104/pp.71.3.610.

H(+)-pumping ATPases were detected in microsomal vesicles of oat (Avena sativa L. var Lang) roots using [(14)C]methylamine distribution or quinacrine fluorescent quenching. Methylamine (MeA) accumulation into vesicles and quinacrine quench were specifically dependent on Mg,ATP. Both activities reflected formation...

Separation of two types of electrogenic h-pumping ATPases from oat roots.

Plant physiology

Churchill KA, Holaway B, Sze H.
PMID: 16663344
Plant Physiol. 1983 Dec;73(4):921-8. doi: 10.1104/pp.73.4.921.

Microsomal vesicles of oat roots (Avena sativa var Lang) were separated with a linear dextran (0.5-10%, w/w) or sucrose (25-45%, w/w) gradient to determine the types and membrane identity of proton-pumping ATPases associated with plant membranes. ATPase activity stimulated...

l-Aspartate Transport into Pea Chloroplasts : Kinetic and Inhibitor Evidence for Multiple Transport Systems.

Plant physiology

Werner-Washburne M, Keegstra K.
PMID: 16664220
Plant Physiol. 1985 Jun;78(2):221-7. doi: 10.1104/pp.78.2.221.

The kinetics of l-aspartate transport into pea chloroplasts was studied in the presence and absence of transport inhibitors to determine whether multiple aspartate carriers exist. Transport was measured by the silicone oil centrifugation technique. Reciprocal plots of concentration-dependent transport...

Auxin and Fusicoccin Enhancement of beta-Glucan Synthase in Peas : An Intracellular Enzyme Activity Apparently Modulated by Proton Extrusion.

Plant physiology

Ray PM.
PMID: 16664267
Plant Physiol. 1985 Jul;78(3):466-72. doi: 10.1104/pp.78.3.466.

Fusicoccin (FC), like indoleacetic acid (IAA), causes Golgi-localized beta-1,4-glucan synthase (GS) activity to increase when applied to pea third internode segments whose GS activity has declined after isolation from the plant. This suggests that GS activity is modulated by...

Reversible Effects of Toxin from Helminthosporium maydis Race T on Oxidative Phosphorylation by Mitochondria from Maize.

Plant physiology

Bednarski MA, Izawa S, Scheffer RP.
PMID: 16659889
Plant Physiol. 1977 Apr;59(4):540-5. doi: 10.1104/pp.59.4.540.

Host-selective toxin from Helminthosporium maydis race T inhibited oxidative phosphorylation (AT(32)P formation) and stimulated ATPase activity by mitochondria from male-sterile (T) but not from normal (N) cytoplasm maize (Zea mays L.). Toxin increased the rate of NADH oxidation, but...

Effect of Osmotic Stress on Ion Transport Processes and Phospholipid Composition of Wheat (Triticum aestivum L.) Mitochondria.

Plant physiology

Klein RR, Burke JJ, Wilson RF.
PMID: 16665169
Plant Physiol. 1986 Dec;82(4):936-41. doi: 10.1104/pp.82.4.936.

The effect of osmotic stress on wheat (Triticum aestivum L.) mitochondrial activity and phospholipid composition was investigated. Preliminary growth measurements showed that osmotic stress (-0.25 or -0.5 megapascal external water potential) inhibited the rate of shoot dry matter accumulation...

Mechanism of Action of Pseudomonas syringae Phytotoxin, Syringomycin : Stimulation of Red Beet Plasma Membrane ATPase Activity.

Plant physiology

Bidwai AP, Zhang L, Bachmann RC, Takemoto JY.
PMID: 16665211
Plant Physiol. 1987 Jan;83(1):39-43. doi: 10.1104/pp.83.1.39.

Syringomycin, a peptide toxin produced by the phytopathogen Pseudomonas syringae pv syringae preferentially stimulated (2-fold) the vanadate-sensitive ATPase activity associated with the plasma membrane of red beet storage tissue. The toxin had a very slight effect on the tonoplast...

Sugar uptake by maize endosperm suspension cultures.

Plant physiology

Felker FC, Goodwin JC.
PMID: 16666450
Plant Physiol. 1988 Dec;88(4):1235-9. doi: 10.1104/pp.88.4.1235.

Maize (Zea mays L.) endosperm suspension cultures are a useful model system for studying biochemical and physiological events in developing maize endosperm. In this report, sugar uptake by the cultures is characterized. Uptake of (14)C-labeled fructose and l-glucose was...

Showing 1 to 12 of 153 entries