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Showing 1 to 12 of 431 entries
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Immobilization of penicillin G acylase using permeabilized Escherichia coli whole cells within chitosan beads.

Research in pharmaceutical sciences

Bagherinejad MR, Korbekandi H, Tavakoli N, Abedi D.
PMID: 23181084
Res Pharm Sci. 2012 Apr;7(2):79-85.

Entrapment of permeabilized whole cells within a matrix is a common method for immobilization. Chitosan possesses distinct chemical and biological properties, which make it a suitable matrix for entrapment and immobilization of penicillin G acylase (PGA). In the first...

Management of syphilis in HIV-positive individuals.

Sexual health

Cresswell FV, Fisher M.
PMID: 25621462
Sex Health. 2015 Apr;12(2):135-40. doi: 10.1071/SH14168.

Since the turn of the millennium a sustained outbreak of syphilis among men who have sex with men continues, approximately 20-50% of whom have concurrent HIV infection. In this paper we aim to explore the controversies that exist around...

Positional Variance in NMR Crystallography.

Journal of the American Chemical Society

Hofstetter A, Emsley L.
PMID: 28146348
J Am Chem Soc. 2017 Feb 22;139(7):2573-2576. doi: 10.1021/jacs.6b12705. Epub 2017 Feb 08.

We propose a method to quantify positional uncertainties in crystal structures determined by chemical-shift-based NMR crystallography. The method combines molecular dynamics simulations and density functional theory calculations with experimental and computational chemical shift uncertainties. In this manner we find...

Evaluation of Penicillin Hypersensitivity with Penicilloyl Polylysine and Penicillin G.

Indian journal of dermatology, venereology and leprology

Kumar B, Kaur S, Choudhary RR.
PMID: 28145386
Indian J Dermatol Venereol Leprol. 1987 Jul-Aug;53(4):223-226.

A case of phaeohyphomycosis in a three-year-old female child born to consanguineous parents is reported. The causative agent was Exophiala jmmlmei. The lesions were found in skin, subcutaneous tissue, lymph nodes and limb bones, which makes it a rare...

Bilateral papillitis and unilateral focal chorioretinitis as the presenting features of syphilis.

Journal of ophthalmic inflammation and infection

Benson CE, Soliman MK, Knezevic A, Xu DD, Nguyen QD, Do DV.
PMID: 26069511
J Ophthalmic Inflamm Infect. 2015 Jun 05;5:16. doi: 10.1186/s12348-015-0045-0. eCollection 2015.

BACKGROUND: Syphilis is a multisystem bacterial infection caused by Treponema pallidum. The incidence of infection in the United States has risen by more than 75% since the year 2000, when it was at a low of 2.1 per 100,000...

Cephalosporin C acylase and penicillin V acylase formation by Aeromonas sp. ACY 95.

World journal of microbiology & biotechnology

Deshpande BS, Ambedkar SS, Shewale JG.
PMID: 24415313
World J Microbiol Biotechnol. 1996 Jul;12(4):373-8. doi: 10.1007/BF00340214.

Aeromonas sp. ACY 95 produces constitutively and intracellularly a penicillin V acylase at an early stage of fermentation (12 h) and a cephalosporin C acylase at a later stage (36 h). Some penicillins, cephalosporin C and their side chain...

Antibacterial Properties of Metallocenyl-7-ADCA Derivatives and Structure in Complex with CTX-M .

Organometallics

Lewandowski EM, Szczupak Ł, Wong S, Skiba J, Guśpiel A, Solecka J, Vrček V, Kowalski K, Chen Y.
PMID: 29051683
Organometallics. 2017 May 08;36(9):1673-1676. doi: 10.1021/acs.organomet.6b00888. Epub 2017 Feb 01.

A series of six novel metallocenyl-7-ADCA (metallocenyl = ferrocenyl or ruthenocenyl; 7-ADCA = 7-aminodesacetoxycephalosporanic acid) conjugates were synthesized and their antibacterial properties evaluated by biochemical and microbiological assays. The ruthenocene derivatives showed a higher level of inhibition of DD-carboxypeptidase...

Detecting the frequency of aminoglycoside modifying enzyme encoding genes among clinical isolates of methicillin-resistant Staphylococcus aureus.

BioImpacts : BI

Shokravi Z, Mehrad L, Ramazani A.
PMID: 26191502
Bioimpacts. 2015;5(2):87-91. doi: 10.15171/bi.2015.15. Epub 2015 Apr 21.

INTRODUCTION: Methicillin-resistant Staphylococcus aureus (MRSA) plays an important role in causing many serious nosocomial infections. In this study, the antimicrobial susceptibility and the frequency of aminoglycoside modifying enzyme encoding genes among clinical isolates of methicillin-resistant Staphylococcus aureus was investigated...

Isolation, abundance and phylogenetic affiliation of endophytic actinomycetes associated with medicinal plants and screening for their in vitro antimicrobial biosynthetic potential.

Frontiers in microbiology

Passari AK, Mishra VK, Saikia R, Gupta VK, Singh BP.
PMID: 25904906
Front Microbiol. 2015 Apr 07;6:273. doi: 10.3389/fmicb.2015.00273. eCollection 2015.

Microorganisms associated with medicinal plants are of interest as the producers of important bioactive compounds. To date, the diversity of culturable endophytic actinomycetes associated with medicinal plants is in its initial phase of exploration. In this study, 42 endophytic...

Propionibacterium acnes: A Treatable Cause of Constrictive Pericarditis.

Case reports in medicine

Cruz D, Ahmed H, Gandapur Y, Abraham MR.
PMID: 26167179
Case Rep Med. 2015;2015:193272. doi: 10.1155/2015/193272. Epub 2015 Jun 17.

In this case report we share a case of infective Pericarditis caused by Propionibacterium acnes (P. acnes) in an immune-competent, nonsurgical patient. This case and review will illustrate the importance of considering P. acnes as a cause of idiopathic...

Production of immobilized penicillin acylase using aqueous polymer systems for enzyme purification and in situ immobilization.

Enzyme and microbial technology

Guan YH, Lilley TH, Brook AH.
PMID: 11166815
Enzyme Microb Technol. 2001 Feb 01;28(2):218-224. doi: 10.1016/s0141-0229(00)00305-7.

A novel approach for the isolation and purification of penicillin acylase (PA), which couples aqueous two-phase partitioning and enzyme immobilization has been investigated.A PA yield of 90% was achieved by treating E. coli cells with 4% butyl acetate, freeze-thawing...

Site-directed Mutagenesis of the Active Center of Penicillin Acylase from E. coli ATCC 11105.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

Dai MH, Wang ED, Xie Y, Jiang WH, Zhao GP.
PMID: 12114970
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1999;31(5):558-562.

Site-directed mutagenesis and chemical modification were performed at Ser290 of the penicillin G acylase from E. coli ATCC11105. The Ser290 was substituted by Cys or Secys. Wild type and mutant proteins were purified, and the activities and kinetic constants...

Showing 1 to 12 of 431 entries