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In-gel s-pyridylethylation of gel-resolved proteins: whole gel method.

CSH protocols

Simpson RJ.
PMID: 21356843
CSH Protoc. 2008 Jun 01;2008:pdb.prot4604. doi: 10.1101/pdb.prot4604.

INTRODUCTIONThis protocol describes a method for performing reduction and S-alkylation of Coomassie-blue-stained proteins within an intact gel. Reduction is performed with dithiothreitol, and alkylation with 4-vinylpyridine. (Treatment of free cysteines with 4-vinylpyridine yields the S-β-(4-pyridylethyl) cysteinyl derivative.) S-β-(4-pyridylethyl) cysteine-containing...

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Simpson RJ. In-gel s-pyridylethylation of gel-resolved proteins: whole gel method. CSH Protoc. 2008;2008:pdb.prot4604doi: 10.1101/pdb.prot4604.
Simpson, R. J. (2008). In-gel s-pyridylethylation of gel-resolved proteins: whole gel method. CSH protocols, 2008pdb.prot4604. https://doi.org/10.1101/pdb.prot4604
Simpson, Richard J. "In-gel s-pyridylethylation of gel-resolved proteins: whole gel method." CSH protocols vol. 2008 (2008): pdb.prot4604. doi: https://doi.org/10.1101/pdb.prot4604
Simpson RJ. In-gel s-pyridylethylation of gel-resolved proteins: whole gel method. CSH Protoc. 2008 Jun 01;2008:pdb.prot4604. doi: 10.1101/pdb.prot4604. PMID: 21356843.
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Application of a coupled reducing system to Edman sequencing.

Journal of biomolecular techniques : JBT

Rusnak F, Hathaway G.
PMID: 19499071
J Biomol Tech. 2001 Sep;12(3):40-3.

Addition of low levels of solid tris-(2-carboxyethyl)-phosphine hydrochloride to Applied Biosystems' R4A (2 mM) and R5 (0.17 mM) resulted in a coupled, phosphinedithiothreitol reducing system. Coupling the two reductants eliminated or greatly decreased interference from the oxidation product of...

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Rusnak F, Hathaway G. Application of a coupled reducing system to Edman sequencing. J Biomol Tech. 2001;12(3):40-3
Rusnak, F., & Hathaway, G. (2001). Application of a coupled reducing system to Edman sequencing. Journal of biomolecular techniques : JBT, 12(3), 40-3.
Rusnak, F, and Hathaway, G. "Application of a coupled reducing system to Edman sequencing." Journal of biomolecular techniques : JBT vol. 12,3 (2001): 40-3.
Rusnak F, Hathaway G. Application of a coupled reducing system to Edman sequencing. J Biomol Tech. 2001 Sep;12(3):40-3. PMID: 19499071; PMCID: PMC2291652.
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Mass spectral identification and quantification of phenylthiohydantoin derivatives from Edman degradation of proteins: Cysteine derivatives.

FEBS letters

Tschesche H, Schneider M, Wachter E.
PMID: 11946655
FEBS Lett. 1972 Jul 01;23(3):367-372. doi: 10.1016/0014-5793(72)80318-1.

No abstract available.

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Tschesche H, Schneider M, Wachter E. Mass spectral identification and quantification of phenylthiohydantoin derivatives from Edman degradation of proteins: Cysteine derivatives. FEBS Lett. 1972;23(3):367-372doi: 10.1016/0014-5793(72)80318-1.
Tschesche, H., Schneider, M., & Wachter, E. (1972). Mass spectral identification and quantification of phenylthiohydantoin derivatives from Edman degradation of proteins: Cysteine derivatives. FEBS letters, 23(3), 367-372. https://doi.org/10.1016/0014-5793(72)80318-1
Tschesche, H, et al. "Mass spectral identification and quantification of phenylthiohydantoin derivatives from Edman degradation of proteins: Cysteine derivatives." FEBS letters vol. 23,3 (1972): 367-372. doi: https://doi.org/10.1016/0014-5793(72)80318-1
Tschesche H, Schneider M, Wachter E. Mass spectral identification and quantification of phenylthiohydantoin derivatives from Edman degradation of proteins: Cysteine derivatives. FEBS Lett. 1972 Jul 01;23(3):367-372. doi: 10.1016/0014-5793(72)80318-1. PMID: 11946655.
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Determination of amino acids in Sargassum fusiforme by high performance capillary electrophoresis.

Talanta

Chen F, Wang S, Guo W, Hu M.
PMID: 18970049
Talanta. 2005 Apr 30;66(3):755-61. doi: 10.1016/j.talanta.2004.12.038. Epub 2005 Jan 18.

High performance capillary electrophoresis (HPCE) was developed for quantitative determination of 18 phenylthiohydantoin (PTH)-amino acids. The influence of electrolyte concentration, pH, organic modifier and applied voltage on HPCE performance was investigated. The HPCE separation of a PTH-amino acids mixture...

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Chen F, Wang S, Guo W, et al. Determination of amino acids in Sargassum fusiforme by high performance capillary electrophoresis. Talanta. 2005;66(3):755-61doi: 10.1016/j.talanta.2004.12.038.
Chen, F., Wang, S., Guo, W., & Hu, M. (2005). Determination of amino acids in Sargassum fusiforme by high performance capillary electrophoresis. Talanta, 66(3), 755-61. https://doi.org/10.1016/j.talanta.2004.12.038
Chen, Fan, et al. "Determination of amino acids in Sargassum fusiforme by high performance capillary electrophoresis." Talanta vol. 66,3 (2005): 755-61. doi: https://doi.org/10.1016/j.talanta.2004.12.038
Chen F, Wang S, Guo W, Hu M. Determination of amino acids in Sargassum fusiforme by high performance capillary electrophoresis. Talanta. 2005 Apr 30;66(3):755-61. doi: 10.1016/j.talanta.2004.12.038. Epub 2005 Jan 18. PMID: 18970049.
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Miniaturized protein microsequencer with PTH amino acid identification by capillary electrophoresis I. An argon pressurized delivery system for adsorptive and covalent sequencing.

Talanta

Waldron KC, Li XF, Chen M, Ireland I, Lewis D, Carpenter M, Dovichi NJ.
PMID: 18966756
Talanta. 1997 Mar;44(3):383-99. doi: 10.1016/s0039-9140(96)02077-2.

The design of a simple, highly miniaturized instrument for manual microsequence analysis of proteins and peptides is described. The reaction chamber is made of fused silica capillary tubing with all reagents and solvents necessary for coupling and cleavage delivered...

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Waldron KC, Li XF, Chen M, et al. Miniaturized protein microsequencer with PTH amino acid identification by capillary electrophoresis I. An argon pressurized delivery system for adsorptive and covalent sequencing. Talanta. 1997;44(3):383-99doi: 10.1016/s0039-9140(96)02077-2.
Waldron, K. C., Li, X. F., Chen, M., Ireland, I., Lewis, D., Carpenter, M., & Dovichi, N. J. (1997). Miniaturized protein microsequencer with PTH amino acid identification by capillary electrophoresis I. An argon pressurized delivery system for adsorptive and covalent sequencing. Talanta, 44(3), 383-99. https://doi.org/10.1016/s0039-9140(96)02077-2
Waldron, K C, et al. "Miniaturized protein microsequencer with PTH amino acid identification by capillary electrophoresis I. An argon pressurized delivery system for adsorptive and covalent sequencing." Talanta vol. 44,3 (1997): 383-99. doi: https://doi.org/10.1016/s0039-9140(96)02077-2
Waldron KC, Li XF, Chen M, Ireland I, Lewis D, Carpenter M, Dovichi NJ. Miniaturized protein microsequencer with PTH amino acid identification by capillary electrophoresis I. An argon pressurized delivery system for adsorptive and covalent sequencing. Talanta. 1997 Mar;44(3):383-99. doi: 10.1016/s0039-9140(96)02077-2. PMID: 18966756.
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In-gel s-pyridylethylation of gel-resolved proteins: individual spot method.

CSH protocols

Simpson RJ.
PMID: 21356844
CSH Protoc. 2008 Jun 01;2008:pdb.prot4605. doi: 10.1101/pdb.prot4605.

INTRODUCTIONThis protocol describes a method for performing reduction and S-alkylation of Coomassie-blue-stained proteins within individually excised, stained protein spots or bands. Reduction is performed with dithiothreitol, and alkylation with 4-vinylpyridine. (Treatment of free cysteines with 4-vinylpyridine yields the S-β-(4-pyridylethyl)...

Cite
Simpson RJ. In-gel s-pyridylethylation of gel-resolved proteins: individual spot method. CSH Protoc. 2008;2008:pdb.prot4605doi: 10.1101/pdb.prot4605.
Simpson, R. J. (2008). In-gel s-pyridylethylation of gel-resolved proteins: individual spot method. CSH protocols, 2008pdb.prot4605. https://doi.org/10.1101/pdb.prot4605
Simpson, Richard J. "In-gel s-pyridylethylation of gel-resolved proteins: individual spot method." CSH protocols vol. 2008 (2008): pdb.prot4605. doi: https://doi.org/10.1101/pdb.prot4605
Simpson RJ. In-gel s-pyridylethylation of gel-resolved proteins: individual spot method. CSH Protoc. 2008 Jun 01;2008:pdb.prot4605. doi: 10.1101/pdb.prot4605. PMID: 21356844.
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Absolute configuration, stability, and interconversion of 6,7-dihydro-7-hydroxy-1-hydroxymethyl-5H-pyrrolizine valine adducts and their phenylthiohydantoin derivatives.

Journal of food and drug analysis

Jiang X, Wang S, Zhao Y, Xia Q, Cai L, Sun X, Fu PP.
PMID: 28911388
J Food Drug Anal. 2015 Jun;23(2):318-326. doi: 10.1016/j.jfda.2015.01.004. Epub 2015 Feb 18.

Pyrrolizidine alkaloid-containing plants are widespread in the world and probably the most common poisonous plants affecting livestock, wildlife, and humans. Pyrrolizidine alkaloids require metabolic activation to form dehydropyrrolizidine alkaloids that bind to cellular proteins and DNA leading to hepatotoxicity,...

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Jiang X, Wang S, Zhao Y, et al. Absolute configuration, stability, and interconversion of 6,7-dihydro-7-hydroxy-1-hydroxymethyl-5H-pyrrolizine valine adducts and their phenylthiohydantoin derivatives. J Food Drug Anal. 2015;23(2):318-326doi: 10.1016/j.jfda.2015.01.004.
Jiang, X., Wang, S., Zhao, Y., Xia, Q., Cai, L., Sun, X., & Fu, P. P. (2015). Absolute configuration, stability, and interconversion of 6,7-dihydro-7-hydroxy-1-hydroxymethyl-5H-pyrrolizine valine adducts and their phenylthiohydantoin derivatives. Journal of food and drug analysis, 23(2), 318-326. https://doi.org/10.1016/j.jfda.2015.01.004
Jiang, Xiao, et al. "Absolute configuration, stability, and interconversion of 6,7-dihydro-7-hydroxy-1-hydroxymethyl-5H-pyrrolizine valine adducts and their phenylthiohydantoin derivatives." Journal of food and drug analysis vol. 23,2 (2015): 318-326. doi: https://doi.org/10.1016/j.jfda.2015.01.004
Jiang X, Wang S, Zhao Y, Xia Q, Cai L, Sun X, Fu PP. Absolute configuration, stability, and interconversion of 6,7-dihydro-7-hydroxy-1-hydroxymethyl-5H-pyrrolizine valine adducts and their phenylthiohydantoin derivatives. J Food Drug Anal. 2015 Jun;23(2):318-326. doi: 10.1016/j.jfda.2015.01.004. Epub 2015 Feb 18. PMID: 28911388.
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Showing 1 to 7 of 7 entries