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Showing 1 to 12 of 64 entries
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Staphylococcal Protein A Promotes Colonization and Immune Evasion of the Epidemic Healthcare-Associated MRSA ST239.

Frontiers in microbiology

Hong X, Qin J, Li T, Dai Y, Wang Y, Liu Q, He L, Lu H, Gao Q, Lin Y, Li M.
PMID: 27446000
Front Microbiol. 2016 Jun 27;7:951. doi: 10.3389/fmicb.2016.00951. eCollection 2016.

The highly successful epidemic of healthcare-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) ST239 is a growing concern worldwide, due to its progressive adaptation to the highly selective environment of the healthcare system. HA-MRSA ST239 display the reduced virulence and successfully colonize...

Expression of an active spinach acyl carrier protein-I/protein-A gene fusion.

Plant molecular biology

Beremand PD, Elmore DD, Dziewanowska K, Guerra DJ.
PMID: 24272721
Plant Mol Biol. 1989 Jan;12(1):95-104. doi: 10.1007/BF00017452.

A synthetic gene encoding spinach acyl carrier protein I (ACP-I) was fused to a gene encoding the Fc-binding portion of staphylococcal protein A. This gene fusion, under the control of the λPR promoter, was expressed at high levels in...

Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide.

BMC biotechnology

Rigi G, Rostami A, Ghomi H, Ahmadian G, Mirbagheri VS, Jeiranikhameneh M, Vahed M, Rahimi S.
PMID: 34399745
BMC Biotechnol. 2021 Aug 16;21(1):51. doi: 10.1186/s12896-021-00701-x.

BACKGROUND: Human Growth Hormone (hGH) is a glycoprotein released from the pituitary gland. Due to the wide range of effects in humans, any disruption in hGH secretion could have serious consequences. This highlights the clinical importance of hGH production...

Molecular epidemiology and phenotypes of invasive methicillin-resistant vancomycin-intermediate Staphylococcus aureus in Taiwan.

Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi

Wang WY, Chiu CF, Lee YT, Hsueh PR, Tsao SM.
PMID: 34635425
J Microbiol Immunol Infect. 2021 Oct 01; doi: 10.1016/j.jmii.2021.09.003. Epub 2021 Oct 01.

BACKGROUND: Patients with invasive infections caused by methicillin-resistant Staphylococcus aureus (MRSA), especially those with an elevated minimal inhibitory concentration (MIC) of vancomycin (VA), are likely to have treatment failure and poor outcomes. The aim of this study was to...

Fusidic Acid Resistance Determinants in Methicillin-Resistant Staphylococcus aureus Isolated in Kuwait Hospitals.

Medical principles and practice : international journal of the Kuwait University, Health Science Centre

Boloki HA, Al-Musaileem WF, AlFouzan W, Verghese T, Udo EE.
PMID: 34348297
Med Princ Pract. 2021;30(6):542-549. doi: 10.1159/000518408. Epub 2021 Jul 13.

OBJECTIVE: The aim of this study was to investigate the genetic determinants of fusidic acid (FA) resistance in MRSA isolated from patients in Kuwait hospitals.METHODS: The minimum inhibitory concentration (MIC) of FA was tested with E-test strips. Genetic determinants...

An Electrochemical Immunosensor Based on SPA and rGO-PEI-Ag-Nf for the Detection of Arsanilic Acid.

Molecules (Basel, Switzerland)

Wang Y, Ma D, Zhang G, Wang X, Zhou J, Chen Y, You X, Liang C, Qi Y, Li Y, Wang A.
PMID: 35011402
Molecules. 2021 Dec 28;27(1). doi: 10.3390/molecules27010172.

A sensitive electrochemical immunosensor was prepared for rapid detection of ASA based on arsanilic acid (ASA) monoclonal antibody with high affinity. In the preparation of nanomaterials, polyethyleneimine (PEI) improved the stability of the solution and acted as a reducing...

Applications of Therapeutic Apheresis in Patients with Malignant Disease.

The oncologist

Pineda AA, Vamvakas EC.
PMID: 10388036
Oncologist. 1997;2(2):94-103.

Therapeutic apheresis (TA) provides the means for the removal of blood components that are abnormal or that circulate in excessive amounts and have a defined pathogenetic role, or are thought to have one. Multiple disease processes have been treated...

Instantaneous Reproductive Effort in Female American Oysters, Crassostrea virginica, Measured by a New Immunoprecipitation Assay.

The Biological bulletin

Choi KS, Powell EN, Lewis DH, Ray SM.
PMID: 29283295
Biol Bull. 1994 Feb;186(1):41-61. doi: 10.2307/1542035.

An immunoprecipitation assay was developed for measuring instantaneous reproductive effort in female American oysters, Crassostrea virginica. Oysters were injected with 14C-leucine and incubated in situ for 1 to 30 h periodically throughout the annual gametogenic cycle. Gonadal protein labeled...

Isolation of Asian endemic and livestock associated clones of methicillin resistant Staphylococcus aureus from ocular samples in Northeastern Iran.

Iranian journal of microbiology

Havaei SA, Azimian A, Fazeli H, Naderi M, Ghazvini K, Samiee SM, Soleimani M.
PMID: 24475328
Iran J Microbiol. 2013 Sep;5(3):227-32.

BACKGROUND AND OBJECTIVES: Methicillin Resistant Staphylococcus aureus (MRSA) strains are divided into Community Associated (CA-) and Hospital Associated (HA-) MRSA. These strains vary in antimicrobial resistance and pathogenicity. S. aureus is one of the most common microorganisms in ocular...

Staphylococcus aureus eye infections in two Indian hospitals: emergence of ST772 as a major clone.

Clinical ophthalmology (Auckland, N.Z.)

Nadig S, Velusamy N, Lalitha P, Kar S, Sharma S, Arakere G.
PMID: 22291460
Clin Ophthalmol. 2012;6:165-73. doi: 10.2147/OPTH.S23878. Epub 2012 Jan 24.

PURPOSE: The purpose of this study was to perform molecular characterization of Staphylococcus aureus isolates causing a variety of eye infections from two major eye care hospitals in India.METHODS: Twenty-four isolates from Aravind Eye Hospital, Madurai, India, and nine...

Enhancement of in vitro interleukin-2 production in normal subjects following a single spinal manipulative treatment.

Chiropractic & osteopathy

Teodorczyk-Injeyan JA, Injeyan HS, McGregor M, Harris GM, Ruegg R.
PMID: 18507834
Chiropr Osteopat. 2008 May 28;16:5. doi: 10.1186/1746-1340-16-5.

BACKGROUND: Increasing evidence supports somato-visceral effects of manual therapies. We have previously demonstrated that a single spinal manipulative treatment (SMT) accompanied by audible release has an inhibitory effect on the production of proinflammatory cytokines in asymptomatic subjects. The purpose...

Engineered protein A ligands, derived from a histidine-scanning library, facilitate the affinity purification of IgG under mild acidic conditions.

Journal of biological engineering

Tsukamoto M, Watanabe H, Ooishi A, Honda S.
PMID: 25057290
J Biol Eng. 2014 Jul 01;8:15. doi: 10.1186/1754-1611-8-15. eCollection 2014.

BACKGROUND: In antibody purification processes, the acidic buffer commonly used to elute the bound antibodies during conventional affinity chromatograph, can damage the antibody. Herein we describe the development of several types of affinity ligands which enable the purification of...

Showing 1 to 12 of 64 entries