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One-Step Reverse Transcription-Polymerase Chain Reaction for Ebola and Marburg Viruses.

Osong public health and research perspectives

Park SW, Lee YJ, Lee WJ, Jee Y, Choi W.
PMID: 27413652
Osong Public Health Res Perspect. 2016 Jun;7(3):205-9. doi: 10.1016/j.phrp.2016.04.004. Epub 2016 May 06.

OBJECTIVES: Ebola and Marburg viruses (EBOVs and MARVs, respectively) are causative agents of severe hemorrhagic fever with high mortality rates in humans and nonhuman primates. In 2014, there was a major Ebola outbreak in various countries in West Africa,...

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Park SW, Lee YJ, Lee WJ, et al. One-Step Reverse Transcription-Polymerase Chain Reaction for Ebola and Marburg Viruses. Osong Public Health Res Perspect. 2016;7(3):205-9doi: 10.1016/j.phrp.2016.04.004.
Park, S. W., Lee, Y. J., Lee, W. J., Jee, Y., & Choi, W. (2016). One-Step Reverse Transcription-Polymerase Chain Reaction for Ebola and Marburg Viruses. Osong public health and research perspectives, 7(3), 205-9. https://doi.org/10.1016/j.phrp.2016.04.004
Park, Sun-Whan, et al. "One-Step Reverse Transcription-Polymerase Chain Reaction for Ebola and Marburg Viruses." Osong public health and research perspectives vol. 7,3 (2016): 205-9. doi: https://doi.org/10.1016/j.phrp.2016.04.004
Park SW, Lee YJ, Lee WJ, Jee Y, Choi W. One-Step Reverse Transcription-Polymerase Chain Reaction for Ebola and Marburg Viruses. Osong Public Health Res Perspect. 2016 Jun;7(3):205-9. doi: 10.1016/j.phrp.2016.04.004. Epub 2016 May 06. PMID: 27413652; PMCID: PMC4927680.
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Sequence-specific Priming and Exonuclease-released Fluorescence Assay for Rapid and Reliable HLA-A Molecular Typing.

Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology

Menon R, Rudert WA, Braun ER, Jaquins-Gerstl A, Faas SJ, Trucco M.
PMID: 10462597
Mol Diagn. 1997 Jun;2(2):99-111. doi: 10.1054/MODI00200099.

Background: The rapid and sensitive method described for low-resolution DNA typing of alleles at the HLA-A locus is based on a sequence-specific primer polymerase chain reaction approach that eliminates post-polymerase chain reaction gel electrophoresis to analyze the results. Methods...

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Menon R, Rudert WA, Braun ER, et al. Sequence-specific Priming and Exonuclease-released Fluorescence Assay for Rapid and Reliable HLA-A Molecular Typing. Mol Diagn. 1997;2(2):99-111doi: 10.1054/MODI00200099.
Menon, R., Rudert, W. A., Braun, E. R., Jaquins-Gerstl, A., Faas, S. J., & Trucco, M. (1997). Sequence-specific Priming and Exonuclease-released Fluorescence Assay for Rapid and Reliable HLA-A Molecular Typing. Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology, 2(2), 99-111. https://doi.org/10.1054/MODI00200099
Menon, et al. "Sequence-specific Priming and Exonuclease-released Fluorescence Assay for Rapid and Reliable HLA-A Molecular Typing." Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology vol. 2,2 (1997): 99-111. doi: https://doi.org/10.1054/MODI00200099
Menon R, Rudert WA, Braun ER, Jaquins-Gerstl A, Faas SJ, Trucco M. Sequence-specific Priming and Exonuclease-released Fluorescence Assay for Rapid and Reliable HLA-A Molecular Typing. Mol Diagn. 1997 Jun;2(2):99-111. doi: 10.1054/MODI00200099. PMID: 10462597.
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The polymerase chain reaction and its uses in endocrinology.

Trends in endocrinology and metabolism: TEM

Eidne KA.
PMID: 18411178
Trends Endocrinol Metab. 1991 Sep-Oct;2(5):169-75. doi: 10.1016/1043-2760(91)90014-e.

The polymerase chain reaction (PCR) represents a major technological development for molecular biologists and will have a great impact on molecular endocrinology. It is a novel technique that amplifies specific DNA sequences with remarkable efficiency. Repeated cycles of denaturation,...

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Eidne KA. The polymerase chain reaction and its uses in endocrinology. Trends Endocrinol Metab. 1991;2(5):169-75doi: 10.1016/1043-2760(91)90014-e.
Eidne, K. A. (1991). The polymerase chain reaction and its uses in endocrinology. Trends in endocrinology and metabolism: TEM, 2(5), 169-75. https://doi.org/10.1016/1043-2760(91)90014-e
Eidne, K A. "The polymerase chain reaction and its uses in endocrinology." Trends in endocrinology and metabolism: TEM vol. 2,5 (1991): 169-75. doi: https://doi.org/10.1016/1043-2760(91)90014-e
Eidne KA. The polymerase chain reaction and its uses in endocrinology. Trends Endocrinol Metab. 1991 Sep-Oct;2(5):169-75. doi: 10.1016/1043-2760(91)90014-e. PMID: 18411178.
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A novel 1297-1304delGCCTGCCA mutation in the exon 10 of the thyroid hormone receptor β gene causes resistance to thyroid hormone.

Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology

Rivolta CM, Gil MS, Ballarino C, Ridruejo MC, Miguel CM, Gimenez SB, Bernacchi SS, Targovnik HM.
PMID: 23315966
Mol Diagn. 2004 Sep;8(3):163-9. doi: 10.1007/BF03260060.

INTRODUCTION: Resistance to the thyroid hormone (RTH) is an inherited syndrome of reduced tissue responsiveness to hormonal action caused by mutations located in the ligand-binding domain and adjacent hinge region of the thyroid hormone receptor β (TRβ) gene.PATIENT: The...

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Rivolta CM, Gil MS, Ballarino C, et al. A novel 1297-1304delGCCTGCCA mutation in the exon 10 of the thyroid hormone receptor β gene causes resistance to thyroid hormone. Mol Diagn. 2004;8(3):163-9doi: 10.1007/BF03260060.
Rivolta, C. M., Gil, M. S., Ballarino, C., Ridruejo, M. C., Miguel, C. M., Gimenez, S. B., Bernacchi, S. S., & Targovnik, H. M. (2004). A novel 1297-1304delGCCTGCCA mutation in the exon 10 of the thyroid hormone receptor β gene causes resistance to thyroid hormone. Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology, 8(3), 163-9. https://doi.org/10.1007/BF03260060
Rivolta, Carina M, et al. "A novel 1297-1304delGCCTGCCA mutation in the exon 10 of the thyroid hormone receptor β gene causes resistance to thyroid hormone." Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology vol. 8,3 (2004): 163-9. doi: https://doi.org/10.1007/BF03260060
Rivolta CM, Gil MS, Ballarino C, Ridruejo MC, Miguel CM, Gimenez SB, Bernacchi SS, Targovnik HM. A novel 1297-1304delGCCTGCCA mutation in the exon 10 of the thyroid hormone receptor β gene causes resistance to thyroid hormone. Mol Diagn. 2004 Sep;8(3):163-9. doi: 10.1007/BF03260060. PMID: 23315966.
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Purification and characterization of Taq polymerase: A 9-week biochemistry laboratory project for undergraduate students.

Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology

Bellin RM, Bruno MK, Farrow MA.
PMID: 21567784
Biochem Mol Biol Educ. 2010 Jan;38(1):11-6. doi: 10.1002/bmb.20352.

We have developed a 9-week undergraduate laboratory series focused on the purification and characterization of Thermus aquaticus DNA polymerase (Taq). Our aim was to provide undergraduate biochemistry students with a full-semester continuing project simulating a research-like experience, while having...

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Bellin RM, Bruno MK, Farrow MA. Purification and characterization of Taq polymerase: A 9-week biochemistry laboratory project for undergraduate students. Biochem Mol Biol Educ. 2010;38(1):11-6doi: 10.1002/bmb.20352.
Bellin, R. M., Bruno, M. K., & Farrow, M. A. (2010). Purification and characterization of Taq polymerase: A 9-week biochemistry laboratory project for undergraduate students. Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology, 38(1), 11-6. https://doi.org/10.1002/bmb.20352
Bellin, Robert M, et al. "Purification and characterization of Taq polymerase: A 9-week biochemistry laboratory project for undergraduate students." Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology vol. 38,1 (2010): 11-6. doi: https://doi.org/10.1002/bmb.20352
Bellin RM, Bruno MK, Farrow MA. Purification and characterization of Taq polymerase: A 9-week biochemistry laboratory project for undergraduate students. Biochem Mol Biol Educ. 2010 Jan;38(1):11-6. doi: 10.1002/bmb.20352. PMID: 21567784.
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Mutating Asn-666 to Glu in the O-helix region of the taq DNA polymerase gene.

Research in pharmaceutical sciences

Sadeghi HM, Rajaei R, Moazen F, Rabbani M, Jafarian-Dehkordi A.
PMID: 21589764
Res Pharm Sci. 2010 Jan;5(1):15-9.

Taq DNA polymerase is widely used in laboratories and for this reason many investigators have focused their attention on understanding the role of various regions and amino acids in this enzyme. O-helix is a part of taq polymerase suggested...

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Sadeghi HM, Rajaei R, Moazen F, et al. Mutating Asn-666 to Glu in the O-helix region of the taq DNA polymerase gene. Res Pharm Sci. 2010;5(1):15-9
Sadeghi, H. M., Rajaei, R., Moazen, F., Rabbani, M., & Jafarian-Dehkordi, A. (2010). Mutating Asn-666 to Glu in the O-helix region of the taq DNA polymerase gene. Research in pharmaceutical sciences, 5(1), 15-9.
Sadeghi, H Mir Mohammad, et al. "Mutating Asn-666 to Glu in the O-helix region of the taq DNA polymerase gene." Research in pharmaceutical sciences vol. 5,1 (2010): 15-9.
Sadeghi HM, Rajaei R, Moazen F, Rabbani M, Jafarian-Dehkordi A. Mutating Asn-666 to Glu in the O-helix region of the taq DNA polymerase gene. Res Pharm Sci. 2010 Jan;5(1):15-9. PMID: 21589764; PMCID: PMC3093097.
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A review of therapeutic aptamer conjugates with emphasis on new approaches.

Pharmaceuticals (Basel, Switzerland)

Bruno JG.
PMID: 24276022
Pharmaceuticals (Basel). 2013 Mar 19;6(3):340-57. doi: 10.3390/ph6030340.

The potential to emulate or enhance antibodies with nucleic acid aptamers while lowering costs has prompted development of new aptamer-protein, siRNA, drug, and nanoparticle conjugates. Specific focal points of this review discuss DNA aptamers covalently bound at their 3'...

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Bruno JG. A review of therapeutic aptamer conjugates with emphasis on new approaches. Pharmaceuticals (Basel). 2013;6(3):340-57doi: 10.3390/ph6030340.
Bruno, J. G. (2013). A review of therapeutic aptamer conjugates with emphasis on new approaches. Pharmaceuticals (Basel, Switzerland), 6(3), 340-57. https://doi.org/10.3390/ph6030340
Bruno, John G. "A review of therapeutic aptamer conjugates with emphasis on new approaches." Pharmaceuticals (Basel, Switzerland) vol. 6,3 (2013): 340-57. doi: https://doi.org/10.3390/ph6030340
Bruno JG. A review of therapeutic aptamer conjugates with emphasis on new approaches. Pharmaceuticals (Basel). 2013 Mar 19;6(3):340-57. doi: 10.3390/ph6030340. PMID: 24276022; PMCID: PMC3816688.
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Engineered DNA polymerase improves PCR results for plastid DNA.

Applications in plant sciences

Schori M, Appel M, Kitko A, Showalter AM.
PMID: 25202519
Appl Plant Sci. 2013 Jan 31;1(2). doi: 10.3732/apps.1200519. eCollection 2013 Feb.

PREMISE OF THE STUDY: Secondary metabolites often inhibit PCR and sequencing reactions in extractions from plant material, especially from silica-dried and herbarium material. A DNA polymerase that is tolerant to inhibitors improves PCR results. •METHODS AND RESULTS: A novel...

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Schori M, Appel M, Kitko A, et al. Engineered DNA polymerase improves PCR results for plastid DNA. Appl Plant Sci. 2013;1(2)doi: 10.3732/apps.1200519.
Schori, M., Appel, M., Kitko, A., & Showalter, A. M. (2013). Engineered DNA polymerase improves PCR results for plastid DNA. Applications in plant sciences, 1(2), . https://doi.org/10.3732/apps.1200519
Schori, Melanie, et al. "Engineered DNA polymerase improves PCR results for plastid DNA." Applications in plant sciences vol. 1,2 (2013). doi: https://doi.org/10.3732/apps.1200519
Schori M, Appel M, Kitko A, Showalter AM. Engineered DNA polymerase improves PCR results for plastid DNA. Appl Plant Sci. 2013 Jan 31;1(2). doi: 10.3732/apps.1200519. eCollection 2013 Feb. PMID: 25202519; PMCID: PMC4105373.
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Obstacles of Multiplex Real-Time PCR for Bacterial 16S rDNA: Primer Specifity and DNA Decontamination of Taq Polymerase.

Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie

Philipp S, Huemer HP, Irschick EU, Gassner C.
PMID: 20737013
Transfus Med Hemother. 2010 Feb;37(1):21-28. doi: 10.1159/000265571. Epub 2010 Jan 07.

BACKGROUND: The detection of a broad range of bacteria by PCR is applied for the screening of blood and blood products with special attention to platelet concentrates. For practical use it is desirable that detection systems include Gram-positive, Gram-negative...

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Philipp S, Huemer HP, Irschick EU, et al. Obstacles of Multiplex Real-Time PCR for Bacterial 16S rDNA: Primer Specifity and DNA Decontamination of Taq Polymerase. Transfus Med Hemother. 2010;37(1):21-28doi: 10.1159/000265571.
Philipp, S., Huemer, H. P., Irschick, E. U., & Gassner, C. (2010). Obstacles of Multiplex Real-Time PCR for Bacterial 16S rDNA: Primer Specifity and DNA Decontamination of Taq Polymerase. Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie, 37(1), 21-28. https://doi.org/10.1159/000265571
Philipp, Sebastian, et al. "Obstacles of Multiplex Real-Time PCR for Bacterial 16S rDNA: Primer Specifity and DNA Decontamination of Taq Polymerase." Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie vol. 37,1 (2010): 21-28. doi: https://doi.org/10.1159/000265571
Philipp S, Huemer HP, Irschick EU, Gassner C. Obstacles of Multiplex Real-Time PCR for Bacterial 16S rDNA: Primer Specifity and DNA Decontamination of Taq Polymerase. Transfus Med Hemother. 2010 Feb;37(1):21-28. doi: 10.1159/000265571. Epub 2010 Jan 07. PMID: 20737013; PMCID: PMC2914405.
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Long range PCR.

Methods in molecular medicine

Waggott W.
PMID: 21390778
Methods Mol Med. 1998;16:81-91. doi: 10.1385/0-89603-499-2:81.

Conventional polymerase chain reaction (PCR) enables reliable amplification of 3-4 kb of DNA (1) while attempts at optimisation has enabled 15.6 kb of λ DNA to be amplified (2). The maximum amplifiable length of PCR is limited by the...

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Waggott W. Long range PCR. Methods Mol Med. 1998;16:81-91doi: 10.1385/0-89603-499-2:81.
Waggott, W. (1998). Long range PCR. Methods in molecular medicine, 1681-91. https://doi.org/10.1385/0-89603-499-2:81
Waggott, W. "Long range PCR." Methods in molecular medicine vol. 16 (1998): 81-91. doi: https://doi.org/10.1385/0-89603-499-2:81
Waggott W. Long range PCR. Methods Mol Med. 1998;16:81-91. doi: 10.1385/0-89603-499-2:81. PMID: 21390778.
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Efficient genomic DNA extraction protocol from medicinal rich Passiflora foetida containing high level of polysaccharide and polyphenol.

SpringerPlus

Lade BD, Patil AS, Paikrao HM.
PMID: 25191636
Springerplus. 2014 Aug 23;3:457. doi: 10.1186/2193-1801-3-457. eCollection 2014.

In Present work, the main objective is to develop less time consuming protocol for genomic DNA isolation from leaves of Passiflora foetida. Optimized protocol is cost effective, as it avoided use of expensive liquid nitrogen. The important parameters of...

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Lade BD, Patil AS, Paikrao HM. Efficient genomic DNA extraction protocol from medicinal rich Passiflora foetida containing high level of polysaccharide and polyphenol. Springerplus. 2014;3:457doi: 10.1186/2193-1801-3-457.
Lade, B. D., Patil, A. S., & Paikrao, H. M. (2014). Efficient genomic DNA extraction protocol from medicinal rich Passiflora foetida containing high level of polysaccharide and polyphenol. SpringerPlus, 3457. https://doi.org/10.1186/2193-1801-3-457
Lade, Bipin Deochand, et al. "Efficient genomic DNA extraction protocol from medicinal rich Passiflora foetida containing high level of polysaccharide and polyphenol." SpringerPlus vol. 3 (2014): 457. doi: https://doi.org/10.1186/2193-1801-3-457
Lade BD, Patil AS, Paikrao HM. Efficient genomic DNA extraction protocol from medicinal rich Passiflora foetida containing high level of polysaccharide and polyphenol. Springerplus. 2014 Aug 23;3:457. doi: 10.1186/2193-1801-3-457. eCollection 2014. PMID: 25191636; PMCID: PMC4153872.
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Novel properties of recombinant Sso7d-Taq DNA polymerase purified using aqueous two-phase extraction: Utilities of the enzyme in viral diagnosis.

Biotechnology reports (Amsterdam, Netherlands)

Sundarrajan S, Parambath S, Suresh S, Rao S, Padmanabhan S.
PMID: 30197870
Biotechnol Rep (Amst). 2018 Jun 27;19:e00270. doi: 10.1016/j.btre.2018.e00270. eCollection 2018 Sep.

Using Sso7d from

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Sundarrajan S, Parambath S, Suresh S, et al. Novel properties of recombinant Sso7d-Taq DNA polymerase purified using aqueous two-phase extraction: Utilities of the enzyme in viral diagnosis. Biotechnol Rep (Amst). 2018;19:e00270doi: 10.1016/j.btre.2018.e00270.
Sundarrajan, S., Parambath, S., Suresh, S., Rao, S., & Padmanabhan, S. (2018). Novel properties of recombinant Sso7d-Taq DNA polymerase purified using aqueous two-phase extraction: Utilities of the enzyme in viral diagnosis. Biotechnology reports (Amsterdam, Netherlands), 19e00270. https://doi.org/10.1016/j.btre.2018.e00270
Sundarrajan, Sudarson, et al. "Novel properties of recombinant Sso7d-Taq DNA polymerase purified using aqueous two-phase extraction: Utilities of the enzyme in viral diagnosis." Biotechnology reports (Amsterdam, Netherlands) vol. 19 (2018): e00270. doi: https://doi.org/10.1016/j.btre.2018.e00270
Sundarrajan S, Parambath S, Suresh S, Rao S, Padmanabhan S. Novel properties of recombinant Sso7d-Taq DNA polymerase purified using aqueous two-phase extraction: Utilities of the enzyme in viral diagnosis. Biotechnol Rep (Amst). 2018 Jun 27;19:e00270. doi: 10.1016/j.btre.2018.e00270. eCollection 2018 Sep. PMID: 30197870; PMCID: PMC6127375.
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Showing 1 to 12 of 50 entries