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Electron microscopic examination of the extracellular polymeric substances in anaerobic granular biofilms.

World journal of microbiology & biotechnology

Macleod FA, Guiot SR, Costerton JW.
PMID: 24414896
World J Microbiol Biotechnol. 1995 Sep;11(5):481-5. doi: 10.1007/BF00286356.

Scanning electron microscopy revealed that collapsed extracellular polymeric substances (EPS) surrounded bacteria present in granular sludge. Treatment of granular sludge with whole-cell antiserum and staining with polycationic ferritin demonstrated that bacteria were enveloped by extensive EPS. Antibody stabilization permitted...

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