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Showing 1 to 9 of 9 entries
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NEXT-peak: a normal-exponential two-peak model for peak-calling in ChIP-seq data.

BMC genomics

Kim NK, Jayatillake RV, Spouge JL.
PMID: 23706083
BMC Genomics. 2013 May 25;14:349. doi: 10.1186/1471-2164-14-349.

BACKGROUND: Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) can locate transcription factor binding sites on genomic scale. Although many models and programs are available to call peaks, none has dominated its competition in comparison studies.RESULTS: We propose a rigorous...

Nanobody-based chromatin immunoprecipitation.

Methods in molecular biology (Clifton, N.J.)

Duc TN, Hassanzadeh-Ghassabeh G, Saerens D, Peeters E, Charlier D, Muyldermans S.
PMID: 22886272
Methods Mol Biol. 2012;911:491-505. doi: 10.1007/978-1-61779-968-6_31.

Chromatin immunoprecipitation (ChIP), followed by microarray hybridization (ChIP-chip) or high-throughput sequencing (ChIP-seq), is becoming a widely used powerful method for the analysis of the in vivo DNA-protein interactions at genomic scale.The success of ChIP largely depends on the quality...

Analyzing ChIP-chip data using bioconductor.

PLoS computational biology

Toedling J, Huber W.
PMID: 19043553
PLoS Comput Biol. 2008 Nov;4(11):e1000227. doi: 10.1371/journal.pcbi.1000227. Epub 2008 Nov 28.

No abstract available.

A statistical framework for power calculations in ChIP-seq experiments.

Bioinformatics (Oxford, England)

Zuo C, Keleş S.
PMID: 23665773
Bioinformatics. 2014 Mar 15;30(6):753-60. doi: 10.1093/bioinformatics/btt200. Epub 2013 May 10.

MOTIVATION: ChIP-seq technology enables investigators to study genome-wide binding of transcription factors and mapping of epigenomic marks. Although the availability of basic analysis tools for ChIP-seq data is rapidly increasing, there has not been much progress on the related...

Chromatin immunoprecipitation assay.

BioTechniques

Das PM, Ramachandran K, vanWert J, Singal R.
PMID: 15597545
Biotechniques. 2004 Dec;37(6):961-9. doi: 10.2144/04376RV01.

Association between proteins and DNA is crucial for many vital cellular functions such as gene transcription, DNA replication and recombination, repair, segregation, chromosomal stability, cell cycle progression, and epigenetic silencing. It is important to know the genomic targets of...

CoCAS: a ChIP-on-chip analysis suite.

Bioinformatics (Oxford, England)

Benoukraf T, Cauchy P, Fenouil R, Jeanniard A, Koch F, Jaeger S, Thieffry D, Imbert J, Andrau JC, Spicuglia S, Ferrier P.
PMID: 19193731
Bioinformatics. 2009 Apr 01;25(7):954-5. doi: 10.1093/bioinformatics/btp075. Epub 2009 Feb 04.

MOTIVATION: High-density tiling microarrays are increasingly used in combination with ChIP assays to study transcriptional regulation. To ease the analysis of the large amounts of data generated by this approach, we have developed ChIP-on-chip Analysis Suite (CoCAS), a standalone...

Reusable, extensible, and modifiable R scripts and Kepler workflows for comprehensive single set ChIP-seq analysis.

BMC bioinformatics

Cormier N, Kolisnik T, Bieda M.
PMID: 27377783
BMC Bioinformatics. 2016 Jul 05;17(1):270. doi: 10.1186/s12859-016-1125-3.

BACKGROUND: There has been an enormous expansion of use of chromatin immunoprecipitation followed by sequencing (ChIP-seq) technologies. Analysis of large-scale ChIP-seq datasets involves a complex series of steps and production of several specialized graphical outputs. A number of systems...

Microfluidics Technologies for Low Cell Number Chromatin Immunoprecipitation.

Cold Spring Harbor protocols

Wu AR, Quake SR.
PMID: 26700100
Cold Spring Harb Protoc. 2016 Apr 01;2016(4):pdb.prot084996. doi: 10.1101/pdb.prot084996.

Protein-DNA interactions are responsible for numerous critical cellular events: For example, gene expression and silencing are mediated by transcription factor protein binding and histone protein modifications, and DNA replication and repair rely on site-specific protein binding. Chromatin immunoprecipitation (ChIP)...

Computational analysis of protein-DNA interactions from ChIP-seq data.

Methods in molecular biology (Clifton, N.J.)

Rougemont J, Naef F.
PMID: 21938632
Methods Mol Biol. 2012;786:263-73. doi: 10.1007/978-1-61779-292-2_16.

Chromatin immunoprecipitation experiments followed by ultra-high-throughput sequencing (ChIP-seq) is becoming the method of choice to identify transcription factor binding sites in prokaryotes and eukaryotes in vivo. Here, we review the computational steps that are necessary for analyzing the sequenced...

Showing 1 to 9 of 9 entries