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RNAi Arrives at the Bedside after a Mere Two Decades.

Molecular therapy : the journal of the American Society of Gene Therapy

Haussecker D.
PMID: 30366820
Mol Ther. 2018 Nov 07;26(11):2533-2534. doi: 10.1016/j.ymthe.2018.10.003. Epub 2018 Oct 23.

No abstract available.

IdealKnock: A framework for efficiently identifying knockout strategies leading to targeted overproduction.

Computational biology and chemistry

Gu D, Zhang C, Zhou S, Wei L, Hua Q.
PMID: 26948338
Comput Biol Chem. 2016 Apr;61:229-37. doi: 10.1016/j.compbiolchem.2016.02.014. Epub 2016 Feb 17.

In recent years, computer aided redesigning methods based on genome-scale metabolic network models (GEMs) have played important roles in metabolic engineering studies; however, most of these methods are hindered by intractable computing times. In particular, methods that predict knockout...

RNAi/CRISPR Screens: from a Pool to a Valid Hit.

Trends in biotechnology

Schuster A, Erasimus H, Fritah S, Nazarov PV, van Dyck E, Niclou SP, Golebiewska A.
PMID: 30177380
Trends Biotechnol. 2019 Jan;37(1):38-55. doi: 10.1016/j.tibtech.2018.08.002. Epub 2018 Aug 31.

High-throughput genetic screens interfering with gene expression are invaluable tools to identify gene function and phenotype-to-genotype interactions. Implementing such screens in the laboratory is challenging, and the choice between currently available technologies based on RNAi and CRISPR/Cas9 (CRISPR-associated protein...

Showing 1 to 3 of 3 entries