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Showing 1 to 6 of 6 entries
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Isothermal circular-strand-displacement polymerization of DNA and microRNA in digital microfluidic devices.

Analytical and bioanalytical chemistry

Giuffrida MC, Zanoli LM, D'Agata R, Finotti A, Gambari R, Spoto G.
PMID: 25579461
Anal Bioanal Chem. 2015 Feb;407(6):1533-43. doi: 10.1007/s00216-014-8405-4. Epub 2015 Jan 13.

Nucleic-acid amplification is a crucial step in nucleic-acid-sequence-detection assays. The use of digital microfluidic devices to miniaturize amplification techniques reduces the required sample volume and the analysis time and offers new possibilities for process automation and integration in a...

MOIRAI: a compact workflow system for CAGE analysis.

BMC bioinformatics

Hasegawa A, Daub C, Carninci P, Hayashizaki Y, Lassmann T.
PMID: 24884663
BMC Bioinformatics. 2014 May 16;15:144. doi: 10.1186/1471-2105-15-144.

BACKGROUND: Cap analysis of gene expression (CAGE) is a sequencing based technology to capture the 5' ends of RNAs in a biological sample. After mapping, a CAGE peak on the genome indicates the position of an active transcriptional start...

Isolation of enriched small RNA from cell-lysate using on-chip isotachophoresis.

Electrophoresis

Khnouf R, Han CM, Munro SA.
PMID: 31675123
Electrophoresis. 2019 Dec;40(23):3140-3147. doi: 10.1002/elps.201900215. Epub 2019 Nov 14.

In spite of the growing interest in the roles and applications of small RNAs (sRNAs), sRNA isolation methods are inconsistent, tedious, and dependent on the starting number of cells. In this work, we employ ITP to isolate sRNAs from...

[Studies on flavonoids from Derris eriocarpa].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

Wang LX, Wu HG, Zhang H, Lou HY, Liang GY, Jiang WW, Yang ZC, Pan WD.
PMID: 26677702
Zhongguo Zhong Yao Za Zhi. 2015 Aug;40(15):3009-12.

Derris eriocarpa, a traditional Chinese medicine belonging to the family of Leguminosae, is widely distributed mainly over Yunnan, Guangxi and Guizhou of China. Modern pharmacological researches on this herb showed that it had extensive bioactivities, such as promoting urination,...

A Spiral-Helix (3D) Tubing Array That Ensures Ultrahigh-Throughput Single-Cell Sampling.

Analytical chemistry

Wei X, Zhang X, Guo R, Chen ML, Yang T, Xu ZR, Wang JH.
PMID: 31724393
Anal Chem. 2019 Dec 17;91(24):15826-15832. doi: 10.1021/acs.analchem.9b04122. Epub 2019 Nov 25.

ICP-MS is powerful in evaluating elemental species at the single-cell level, where high throughput/efficiency/precision are the keys for achieving statistically significant information based on massive data. We report an ultrahigh-throughput single-cell sampling system, consisting of a 3D spiral-helix tubing...

BAMscale: quantification of next-generation sequencing peaks and generation of scaled coverage tracks.

Epigenetics & chromatin

Pongor LS, Gross JM, Vera Alvarez R, Murai J, Jang SM, Zhang H, Redon C, Fu H, Huang SY, Thakur B, Baris A, Marino-Ramirez L, Landsman D, Aladjem MI, Pommier Y.
PMID: 32321568
Epigenetics Chromatin. 2020 Apr 22;13(1):21. doi: 10.1186/s13072-020-00343-x.

BACKGROUND: Next-generation sequencing allows genome-wide analysis of changes in chromatin states and gene expression. Data analysis of these increasingly used methods either requires multiple analysis steps, or extensive computational time. We sought to develop a tool for rapid quantification...

Showing 1 to 6 of 6 entries