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Effect of age on pro-inflammatory miRNAs contained in mesenchymal stem cell-derived extracellular vesicles.

Scientific reports

Fafián-Labora J, Lesende-Rodriguez I, Fernández-Pernas P, Sangiao-Alvarellos S, Monserrat L, Arntz OJ, van de Loo FJ, Mateos J, Arufe MC.
PMID: 28262816
Sci Rep. 2017 Mar 06;7:43923. doi: 10.1038/srep43923.

Stem cells possess significant age-dependent differences in their immune-response profile. These differences were analysed by Next-Generation Sequencing of six age groups from bone marrow mesenchymal stem cells. A total of 9,628 genes presenting differential expression between age groups were...

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Fafián-Labora J, Lesende-Rodriguez I, Fernández-Pernas P, et al. Effect of age on pro-inflammatory miRNAs contained in mesenchymal stem cell-derived extracellular vesicles. Sci Rep. 2017;7:43923doi: 10.1038/srep43923.
Fafián-Labora, J., Lesende-Rodriguez, I., Fernández-Pernas, P., Sangiao-Alvarellos, S., Monserrat, L., Arntz, O. J., van de Loo, F. J., Mateos, J., & Arufe, M. C. (2017). Effect of age on pro-inflammatory miRNAs contained in mesenchymal stem cell-derived extracellular vesicles. Scientific reports, 743923. https://doi.org/10.1038/srep43923
Fafián-Labora, J, et al. "Effect of age on pro-inflammatory miRNAs contained in mesenchymal stem cell-derived extracellular vesicles." Scientific reports vol. 7 (2017): 43923. doi: https://doi.org/10.1038/srep43923
Fafián-Labora J, Lesende-Rodriguez I, Fernández-Pernas P, Sangiao-Alvarellos S, Monserrat L, Arntz OJ, van de Loo FJ, Mateos J, Arufe MC. Effect of age on pro-inflammatory miRNAs contained in mesenchymal stem cell-derived extracellular vesicles. Sci Rep. 2017 Mar 06;7:43923. doi: 10.1038/srep43923. PMID: 28262816; PMCID: PMC5338265.
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Real -time PCR for early microbiological diagnosis: is it time?.

Intensive care medicine

Paiva JA, Laupland KB.
PMID: 28536877
Intensive Care Med. 2017 Nov;43(11):1714-1716. doi: 10.1007/s00134-017-4793-1. Epub 2017 May 23.

No abstract available.

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Paiva JA, Laupland KB. Real -time PCR for early microbiological diagnosis: is it time?. Intensive Care Med. 2017;43(11):1714-1716doi: 10.1007/s00134-017-4793-1.
Paiva, J. A., & Laupland, K. B. (2017). Real -time PCR for early microbiological diagnosis: is it time?. Intensive care medicine, 43(11), 1714-1716. https://doi.org/10.1007/s00134-017-4793-1
Paiva, José-Artur, and Laupland, Kevin B. "Real -time PCR for early microbiological diagnosis: is it time?." Intensive care medicine vol. 43,11 (2017): 1714-1716. doi: https://doi.org/10.1007/s00134-017-4793-1
Paiva JA, Laupland KB. Real -time PCR for early microbiological diagnosis: is it time?. Intensive Care Med. 2017 Nov;43(11):1714-1716. doi: 10.1007/s00134-017-4793-1. Epub 2017 May 23. PMID: 28536877; PMCID: PMC7095199.
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[Validation of the libraries of the serial analysis of gene expression by the application of real-time quantitative polymerase chain reaction].

Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae

Yin LM, Zhang QH, Wang Y, Xu YD, Yang YQ.
PMID: 20236588
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2010 Feb;32(1):51-4. doi: 10.3881/j.issn.1000-503X.2010.01.013.

OBJECTIVE: To validate and supplement the libraries of serial analysis of gene expression (SAGE) by the application of the real-time quantitative polymerase chain reaction PCR).METHODS: The primers were designed based on the full sequences of the genes. Nine single...

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Yin LM, Zhang QH, Wang Y, et al. [Validation of the libraries of the serial analysis of gene expression by the application of real-time quantitative polymerase chain reaction]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2010;32(1):51-4doi: 10.3881/j.issn.1000-503X.2010.01.013.
Yin, L. M., Zhang, Q. H., Wang, Y., Xu, Y. D., & Yang, Y. Q. (2010). [Validation of the libraries of the serial analysis of gene expression by the application of real-time quantitative polymerase chain reaction]. Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae, 32(1), 51-4. https://doi.org/10.3881/j.issn.1000-503X.2010.01.013
Yin, Lei-miao, et al. "[Validation of the libraries of the serial analysis of gene expression by the application of real-time quantitative polymerase chain reaction]." Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae vol. 32,1 (2010): 51-4. doi: https://doi.org/10.3881/j.issn.1000-503X.2010.01.013
Yin LM, Zhang QH, Wang Y, Xu YD, Yang YQ. [Validation of the libraries of the serial analysis of gene expression by the application of real-time quantitative polymerase chain reaction]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2010 Feb;32(1):51-4. doi: 10.3881/j.issn.1000-503X.2010.01.013. Chinese. PMID: 20236588.
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Differential gene expression in HIV-infected individuals following ART.

Antiviral research

Massanella M, Singhania A, Beliakova-Bethell N, Pier R, Lada SM, White CH, Pérez-Santiago J, Blanco J, Richman DD, Little SJ, Woelk CH.
PMID: 23933117
Antiviral Res. 2013 Nov;100(2):420-8. doi: 10.1016/j.antiviral.2013.07.017. Epub 2013 Aug 06.

Previous studies of the effect of ART on gene expression in HIV-infected individuals have identified small numbers of modulated genes. Since these studies were underpowered or cross-sectional in design, a paired analysis of peripheral blood mononuclear cells (PBMCs), isolated...

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Massanella M, Singhania A, Beliakova-Bethell N, et al. Differential gene expression in HIV-infected individuals following ART. Antiviral Res. 2013;100(2):420-8doi: 10.1016/j.antiviral.2013.07.017.
Massanella, M., Singhania, A., Beliakova-Bethell, N., Pier, R., Lada, S. M., White, C. H., Pérez-Santiago, J., Blanco, J., Richman, D. D., Little, S. J., & Woelk, C. H. (2013). Differential gene expression in HIV-infected individuals following ART. Antiviral research, 100(2), 420-8. https://doi.org/10.1016/j.antiviral.2013.07.017
Massanella, Marta, et al. "Differential gene expression in HIV-infected individuals following ART." Antiviral research vol. 100,2 (2013): 420-8. doi: https://doi.org/10.1016/j.antiviral.2013.07.017
Massanella M, Singhania A, Beliakova-Bethell N, Pier R, Lada SM, White CH, Pérez-Santiago J, Blanco J, Richman DD, Little SJ, Woelk CH. Differential gene expression in HIV-infected individuals following ART. Antiviral Res. 2013 Nov;100(2):420-8. doi: 10.1016/j.antiviral.2013.07.017. Epub 2013 Aug 06. PMID: 23933117; PMCID: PMC3899918.
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A novel picoliter droplet array for parallel real-time polymerase chain reaction based on double-inkjet printing.

Lab on a chip

Sun Y, Zhou X, Yu Y.
PMID: 25070461
Lab Chip. 2014 Sep 21;14(18):3603-10. doi: 10.1039/c4lc00598h. Epub 2014 Jul 29.

We developed and characterized a novel picoliter droplet-in-oil array generated by a double-inkjet printing method on a uniform hydrophobic silicon chip specifically designed for quantitative polymerase chain reaction (qPCR) analysis. Double-inkjet printing was proposed to efficiently address the evaporation...

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Sun Y, Zhou X, Yu Y. A novel picoliter droplet array for parallel real-time polymerase chain reaction based on double-inkjet printing. Lab Chip. 2014;14(18):3603-10doi: 10.1039/c4lc00598h.
Sun, Y., Zhou, X., & Yu, Y. (2014). A novel picoliter droplet array for parallel real-time polymerase chain reaction based on double-inkjet printing. Lab on a chip, 14(18), 3603-10. https://doi.org/10.1039/c4lc00598h
Sun, Yingnan, et al. "A novel picoliter droplet array for parallel real-time polymerase chain reaction based on double-inkjet printing." Lab on a chip vol. 14,18 (2014): 3603-10. doi: https://doi.org/10.1039/c4lc00598h
Sun Y, Zhou X, Yu Y. A novel picoliter droplet array for parallel real-time polymerase chain reaction based on double-inkjet printing. Lab Chip. 2014 Sep 21;14(18):3603-10. doi: 10.1039/c4lc00598h. Epub 2014 Jul 29. PMID: 25070461.
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A home-brew real-time PCR assay for reliable detection and quantification of mature miR-122.

Applied immunohistochemistry & molecular morphology : AIMM

Naderi M, Abdul Tehrani H, Soleimani M, Shabani I, Hashemi SM.
PMID: 25075470
Appl Immunohistochem Mol Morphol. 2015 Sep;23(8):601-6. doi: 10.1097/PAI.0000000000000125.

miR-122 is a liver-specific miRNA that has significant gene expression alterations in response to specific pathophysiological circumstances of liver such as drug-induced liver injury, hepatocellular carcinoma, and hepatitis B and C virus infections. Therefore, accurate and precise quantification of...

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Naderi M, Abdul Tehrani H, Soleimani M, et al. A home-brew real-time PCR assay for reliable detection and quantification of mature miR-122. Appl Immunohistochem Mol Morphol. 2015;23(8):601-6doi: 10.1097/PAI.0000000000000125.
Naderi, M., Abdul Tehrani, H., Soleimani, M., Shabani, I., & Hashemi, S. M. (2015). A home-brew real-time PCR assay for reliable detection and quantification of mature miR-122. Applied immunohistochemistry & molecular morphology : AIMM, 23(8), 601-6. https://doi.org/10.1097/PAI.0000000000000125
Naderi, Mahmood, et al. "A home-brew real-time PCR assay for reliable detection and quantification of mature miR-122." Applied immunohistochemistry & molecular morphology : AIMM vol. 23,8 (2015): 601-6. doi: https://doi.org/10.1097/PAI.0000000000000125
Naderi M, Abdul Tehrani H, Soleimani M, Shabani I, Hashemi SM. A home-brew real-time PCR assay for reliable detection and quantification of mature miR-122. Appl Immunohistochem Mol Morphol. 2015 Sep;23(8):601-6. doi: 10.1097/PAI.0000000000000125. PMID: 25075470.
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Development of a DNA-Based Real-Time PCR Assay To Quantify .

Plant disease

Nguyen-Huu T, Doré J, Aït Barka E, Lavire C, Clément C, Vial L, Sanchez L.
PMID: 32734845
Plant Dis. 2021 Feb;105(2):384-391. doi: 10.1094/PDIS-04-20-0732-RE. Epub 2020 Dec 10.

No abstract available.

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Nguyen-Huu T, Doré J, Aït Barka E, et al. Development of a DNA-Based Real-Time PCR Assay To Quantify . Plant Dis. 2020;105(2):384-391doi: 10.1094/PDIS-04-20-0732-RE.
Nguyen-Huu, T., Doré, J., Aït Barka, E., Lavire, C., Clément, C., Vial, L., & Sanchez, L. (2021). Development of a DNA-Based Real-Time PCR Assay To Quantify . Plant disease, 105(2), 384-391. https://doi.org/10.1094/PDIS-04-20-0732-RE
Nguyen-Huu, Trong, et al. "Development of a DNA-Based Real-Time PCR Assay To Quantify ." Plant disease vol. 105,2 (2021): 384-391. doi: https://doi.org/10.1094/PDIS-04-20-0732-RE
Nguyen-Huu T, Doré J, Aït Barka E, Lavire C, Clément C, Vial L, Sanchez L. Development of a DNA-Based Real-Time PCR Assay To Quantify . Plant Dis. 2021 Feb;105(2):384-391. doi: 10.1094/PDIS-04-20-0732-RE. Epub 2020 Dec 10. PMID: 32734845.
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A low-cost TaqMan minor groove binder probe-based one-step RT-qPCR assay for rapid identification of N501Y variants of SARS-CoV-2.

Journal of virological methods

Chan CT, Leung JS, Lee LK, Lo HW, Wong EY, Wong DS, Ng TT, Lao HY, Lu KK, Jim SH, Yau MC, Lam JY, Ho AY, Luk KS, Yip KT, Que TL, To KK, Siu GK.
PMID: 34656702
J Virol Methods. 2022 Jan;299:114333. doi: 10.1016/j.jviromet.2021.114333. Epub 2021 Oct 14.

The increasing prevalence of N501Y variants of SARS-CoV-2 has kindled global concern due to their enhanced transmissibility. Genome sequencing is the gold standard method to identify the emerging variants of concern. But it is time-consuming and expensive, limiting the...

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Chan CT, Leung JS, Lee LK, et al. A low-cost TaqMan minor groove binder probe-based one-step RT-qPCR assay for rapid identification of N501Y variants of SARS-CoV-2. J Virol Methods. 2021;299:114333doi: 10.1016/j.jviromet.2021.114333.
Chan, C. T., Leung, J. S., Lee, L. K., Lo, H. W., Wong, E. Y., Wong, D. S., Ng, T. T., Lao, H. Y., Lu, K. K., Jim, S. H., Yau, M. C., Lam, J. Y., Ho, A. Y., Luk, K. S., Yip, K. T., Que, T. L., To, K. K., & Siu, G. K. (2022). A low-cost TaqMan minor groove binder probe-based one-step RT-qPCR assay for rapid identification of N501Y variants of SARS-CoV-2. Journal of virological methods, 299114333. https://doi.org/10.1016/j.jviromet.2021.114333
Chan, Chloe Toi-Mei, et al. "A low-cost TaqMan minor groove binder probe-based one-step RT-qPCR assay for rapid identification of N501Y variants of SARS-CoV-2." Journal of virological methods vol. 299 (2022): 114333. doi: https://doi.org/10.1016/j.jviromet.2021.114333
Chan CT, Leung JS, Lee LK, Lo HW, Wong EY, Wong DS, Ng TT, Lao HY, Lu KK, Jim SH, Yau MC, Lam JY, Ho AY, Luk KS, Yip KT, Que TL, To KK, Siu GK. A low-cost TaqMan minor groove binder probe-based one-step RT-qPCR assay for rapid identification of N501Y variants of SARS-CoV-2. J Virol Methods. 2022 Jan;299:114333. doi: 10.1016/j.jviromet.2021.114333. Epub 2021 Oct 14. PMID: 34656702; PMCID: PMC8516123.
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Utility of a commercial RT-qPCR assay to detect SARS-CoV-2 gene variations as an indicator of lineages.

Journal of virological methods

Trobajo-Sanmartín C, Miqueleiz A, Portillo ME, Fernández-Huerta M, Navascués A, Castilla J, Ezpeleta C.
PMID: 34906667
J Virol Methods. 2022 Feb;300:114428. doi: 10.1016/j.jviromet.2021.114428. Epub 2021 Dec 11.

BACKGROUND: The World Health Organization (WHO) recommended RT-qPCR tests as the reference technique for SARS-CoV-2 molecular detection, however with the rapid spread of the infection, mutations in specific RT-qPCR target regions have been widely described could allow the presumptive...

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Trobajo-Sanmartín C, Miqueleiz A, Portillo ME, et al. Utility of a commercial RT-qPCR assay to detect SARS-CoV-2 gene variations as an indicator of lineages. J Virol Methods. 2021;300:114428doi: 10.1016/j.jviromet.2021.114428.
Trobajo-Sanmartín, C., Miqueleiz, A., Portillo, M. E., Fernández-Huerta, M., Navascués, A., Castilla, J., & Ezpeleta, C. (2022). Utility of a commercial RT-qPCR assay to detect SARS-CoV-2 gene variations as an indicator of lineages. Journal of virological methods, 300114428. https://doi.org/10.1016/j.jviromet.2021.114428
Trobajo-Sanmartín, Camino, et al. "Utility of a commercial RT-qPCR assay to detect SARS-CoV-2 gene variations as an indicator of lineages." Journal of virological methods vol. 300 (2022): 114428. doi: https://doi.org/10.1016/j.jviromet.2021.114428
Trobajo-Sanmartín C, Miqueleiz A, Portillo ME, Fernández-Huerta M, Navascués A, Castilla J, Ezpeleta C. Utility of a commercial RT-qPCR assay to detect SARS-CoV-2 gene variations as an indicator of lineages. J Virol Methods. 2022 Feb;300:114428. doi: 10.1016/j.jviromet.2021.114428. Epub 2021 Dec 11. PMID: 34906667; PMCID: PMC8665652.
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Ultrafast and Real-Time Nanoplasmonic On-Chip Polymerase Chain Reaction for Rapid and Quantitative Molecular Diagnostics.

ACS nano

Kang BH, Lee Y, Yu ES, Na H, Kang M, Huh HJ, Jeong KH.
PMID: 34008961
ACS Nano. 2021 Jun 22;15(6):10194-10202. doi: 10.1021/acsnano.1c02154. Epub 2021 May 19.

Advent and fast spread of pandemic diseases draw worldwide attention to rapid, prompt, and accurate molecular diagnostics with technical development of ultrafast polymerase chain reaction (PCR). Microfluidic on-chip PCR platforms provide highly efficient and small-volume bioassay for point-of-care diagnostic...

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Kang BH, Lee Y, Yu ES, et al. Ultrafast and Real-Time Nanoplasmonic On-Chip Polymerase Chain Reaction for Rapid and Quantitative Molecular Diagnostics. ACS Nano. 2021;15(6):10194-10202doi: 10.1021/acsnano.1c02154.
Kang, B. H., Lee, Y., Yu, E. S., Na, H., Kang, M., Huh, H. J., & Jeong, K. H. (2021). Ultrafast and Real-Time Nanoplasmonic On-Chip Polymerase Chain Reaction for Rapid and Quantitative Molecular Diagnostics. ACS nano, 15(6), 10194-10202. https://doi.org/10.1021/acsnano.1c02154
Kang, Byoung-Hoon, et al. "Ultrafast and Real-Time Nanoplasmonic On-Chip Polymerase Chain Reaction for Rapid and Quantitative Molecular Diagnostics." ACS nano vol. 15,6 (2021): 10194-10202. doi: https://doi.org/10.1021/acsnano.1c02154
Kang BH, Lee Y, Yu ES, Na H, Kang M, Huh HJ, Jeong KH. Ultrafast and Real-Time Nanoplasmonic On-Chip Polymerase Chain Reaction for Rapid and Quantitative Molecular Diagnostics. ACS Nano. 2021 Jun 22;15(6):10194-10202. doi: 10.1021/acsnano.1c02154. Epub 2021 May 19. PMID: 34008961.
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Limited capacity of SARS-CoV-2 variants testing in Japan: A secondary analysis using publicly available data.

Travel medicine and infectious disease

Hashimoto T, Ozaki A, Bhandari D, Sawano T, Murayama A, Shrestha S, Sah R, Tanimoto T, Montenegro-Idrogo JJ, Rodriguez-Morales AJ.
PMID: 34298174
Travel Med Infect Dis. 2021 Sep-Oct;43:102145. doi: 10.1016/j.tmaid.2021.102145. Epub 2021 Jul 21.

No abstract available.

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Hashimoto T, Ozaki A, Bhandari D, et al. Limited capacity of SARS-CoV-2 variants testing in Japan: A secondary analysis using publicly available data. Travel Med Infect Dis. 2021;43:102145doi: 10.1016/j.tmaid.2021.102145.
Hashimoto, T., Ozaki, A., Bhandari, D., Sawano, T., Murayama, A., Shrestha, S., Sah, R., Tanimoto, T., Montenegro-Idrogo, J. J., & Rodriguez-Morales, A. J. (2021). Limited capacity of SARS-CoV-2 variants testing in Japan: A secondary analysis using publicly available data. Travel medicine and infectious disease, 43102145. https://doi.org/10.1016/j.tmaid.2021.102145
Hashimoto, Takanao, et al. "Limited capacity of SARS-CoV-2 variants testing in Japan: A secondary analysis using publicly available data." Travel medicine and infectious disease vol. 43 (2021): 102145. doi: https://doi.org/10.1016/j.tmaid.2021.102145
Hashimoto T, Ozaki A, Bhandari D, Sawano T, Murayama A, Shrestha S, Sah R, Tanimoto T, Montenegro-Idrogo JJ, Rodriguez-Morales AJ. Limited capacity of SARS-CoV-2 variants testing in Japan: A secondary analysis using publicly available data. Travel Med Infect Dis. 2021 Sep-Oct;43:102145. doi: 10.1016/j.tmaid.2021.102145. Epub 2021 Jul 21. PMID: 34298174; PMCID: PMC8294103.
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Rapid and simple method of qPCR primer design.

Methods in molecular biology (Clifton, N.J.)

Thornton B, Basu C.
PMID: 25697660
Methods Mol Biol. 2015;1275:173-9. doi: 10.1007/978-1-4939-2365-6_13.

Quantitative real-time polymerase chain reaction (qPCR) is a powerful tool for analysis and quantification of gene expression. It is advantageous compared to traditional gel-based method of PCR, as gene expression can be visualized "real-time" using a computer. In qPCR,...

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Thornton B, Basu C. Rapid and simple method of qPCR primer design. Methods Mol Biol. 2015;1275:173-9doi: 10.1007/978-1-4939-2365-6_13.
Thornton, B., & Basu, C. (2015). Rapid and simple method of qPCR primer design. Methods in molecular biology (Clifton, N.J.), 1275173-9. https://doi.org/10.1007/978-1-4939-2365-6_13
Thornton, Brenda, and Basu, Chhandak. "Rapid and simple method of qPCR primer design." Methods in molecular biology (Clifton, N.J.) vol. 1275 (2015): 173-9. doi: https://doi.org/10.1007/978-1-4939-2365-6_13
Thornton B, Basu C. Rapid and simple method of qPCR primer design. Methods Mol Biol. 2015;1275:173-9. doi: 10.1007/978-1-4939-2365-6_13. PMID: 25697660.
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Showing 1 to 12 of 71 entries