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Fluhr R, Lips SH, Sagi M. Aldehyde oxidase and xanthine dehydrogenase in a flacca tomato mutant with deficient abscisic acid and wilty phenotype . Plant Physiol. 1999;120(2):571-8doi: 10.1104/pp.120.2.571.
Fluhr, R., Lips, S. H., & Sagi, M. (1999). Aldehyde oxidase and xanthine dehydrogenase in a flacca tomato mutant with deficient abscisic acid and wilty phenotype . Plant physiology, 120(2), 571-8. https://doi.org/10.1104/pp.120.2.571
Fluhr, et al. "Aldehyde oxidase and xanthine dehydrogenase in a flacca tomato mutant with deficient abscisic acid and wilty phenotype ." Plant physiology vol. 120,2 (1999): 571-8. doi: https://doi.org/10.1104/pp.120.2.571
Fluhr R, Lips SH, Sagi M. Aldehyde oxidase and xanthine dehydrogenase in a flacca tomato mutant with deficient abscisic acid and wilty phenotype . Plant Physiol. 1999 Jun;120(2):571-8. doi: 10.1104/pp.120.2.571. PMID: 10364409; PMCID: PMC59296.
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PubMed Central Free PMC Article

Plant Physiol. 1999 Jun;120(2):571-8. doi: 10.1104/pp.120.2.571.

Aldehyde oxidase and xanthine dehydrogenase in a flacca tomato mutant with deficient abscisic acid and wilty phenotype .

Plant physiology

Fluhr, Lips, Sagi

Affiliations

  1. Biostress Research Laboratory, Department of Life Science, Faculty of Natural Sciences, Ben-Gurion University of the Negev, Sede Boqer 84990, Israel (M.S., S.H.L.).

PMID: 10364409 PMCID: PMC59296 DOI: 10.1104/pp.120.2.571
Free PMC Article

Abstract

The flacca tomato (Lycopersicon esculentum) mutant displays a wilty phenotype as a result of abscisic acid (ABA) deficiency. The Mo cofactor (MoCo)-containing aldehyde oxidases (AO; EC 1.2.3.1) are thought to play a role in the final oxidation step required for ABA biosynthesis. AO and related MoCo-containing enzymes xanthine dehydrogenase (XDH; EC 1.2.1.37) and nitrate reductase (EC 1.6.6.1) were examined in extracts of the flacca tomato genotype and of wild-type (WT) roots and shoots. The levels of MoCo were found to be similar in both genotypes. No significant XDH or AO (MoCo-containing hydroxylases) activities were detected in flacca leaves; however, the mutant exhibited considerable MoCo-containing hydroxylase activity in the roots, which contained notable amounts of ABA. Native western blots probed with an antibody to MoCo-containing hydroxylases revealed substantial, albeit reduced, levels of cross-reactive protein in the flacca mutant shoots and roots. The ABA xylem-loading rate was significantly lower than that in the WT, indicating that the flacca is also defective in ABA transport to the shoot. Significantly, in vitro sulfurylation with Na2S reactivated preexisting XDH and AO proteins in extracts from flacca, particularly from the shoots, and superinduced the basal-level activity in the WT extracts. The results indicate that in flacca, MoCo-sulfurylase activity is impaired in a tissue-dependent manner.

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