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Collins MA, McConville KJ, McReynolds C, et al. Potential of conjugal transfer as a strategy for the introduction of recombinant genetic material into strains of lactobacillus helveticus . Appl Environ Microbiol. 1999;65(5):1910-4doi: 10.1128/AEM.65.5.1910-1914.1999.
Collins, M. A., McConville, K. J., McReynolds, C., & Thompson, J. K. (1999). Potential of conjugal transfer as a strategy for the introduction of recombinant genetic material into strains of lactobacillus helveticus . Applied and environmental microbiology, 65(5), 1910-4. https://doi.org/10.1128/AEM.65.5.1910-1914.1999
Collins, et al. "Potential of conjugal transfer as a strategy for the introduction of recombinant genetic material into strains of lactobacillus helveticus ." Applied and environmental microbiology vol. 65,5 (1999): 1910-4. doi: https://doi.org/10.1128/AEM.65.5.1910-1914.1999
Collins MA, McConville KJ, McReynolds C, Thompson JK. Potential of conjugal transfer as a strategy for the introduction of recombinant genetic material into strains of lactobacillus helveticus . Appl Environ Microbiol. 1999 May;65(5):1910-4. doi: 10.1128/AEM.65.5.1910-1914.1999. PMID: 10223978; PMCID: PMC91275.
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Appl Environ Microbiol. 1999 May;65(5):1910-4. doi: 10.1128/AEM.65.5.1910-1914.1999.

Potential of conjugal transfer as a strategy for the introduction of recombinant genetic material into strains of lactobacillus helveticus .

Applied and environmental microbiology

Collins, McConville, McReynolds, Thompson

Affiliations

  1. Department of Food Science (Food Microbiology), Department of Agriculture for Northern Ireland.

PMID: 10223978 PMCID: PMC91275 DOI: 10.1128/AEM.65.5.1910-1914.1999
Free PMC Article

Abstract

Cointegrates generated between a plasmid pIP501 deletion derivative (pVA797) and nonconjugative shuttle vector pSA3 were confirmed as capable of exconjugation from lactococci into a range of strains of Lactobacillus helveticus with the concomitant expression of a recombinant gene. The plasmid cointegrate that was formed appeared to be segregationally stable at 37 degrees C in some host strains. In all strains, however, the plasmid became increasingly unstable as the incubation temperature was raised. The technique offers not only a generalized method for the introduction of novel genetic material into this important industrial microbe but also the possibility of exploiting the thermal sensitivity of the plasmid to enable it to act as a delivery system for the integration of cloned genes into the bacterial chromosome, at restrictive temperatures, by recombination at regions of homology.

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