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Mitsuo R, Itakura T, Sato M. Cloning and Sequencing of Cytochrome P450 1A Complementary DNA in Eel (Anguilla japonica). Mar Biotechnol (NY). 1999;1(4):353-358doi: 10.1007/pl00011786.
Mitsuo, R., Itakura, T., & Sato, M. (1999). Cloning and Sequencing of Cytochrome P450 1A Complementary DNA in Eel (Anguilla japonica). Marine biotechnology (New York, N.Y.), 1(4), 353-358. https://doi.org/10.1007/pl00011786
Mitsuo, et al. "Cloning and Sequencing of Cytochrome P450 1A Complementary DNA in Eel (Anguilla japonica)." Marine biotechnology (New York, N.Y.) vol. 1,4 (1999): 353-358. doi: https://doi.org/10.1007/pl00011786
Mitsuo R, Itakura T, Sato M. Cloning and Sequencing of Cytochrome P450 1A Complementary DNA in Eel (Anguilla japonica). Mar Biotechnol (NY). 1999 Jul;1(4):353-358. doi: 10.1007/pl00011786. PMID: 10489411.
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Mar Biotechnol (NY). 1999 Jul;1(4):353-358. doi: 10.1007/pl00011786.

Cloning and Sequencing of Cytochrome P450 1A Complementary DNA in Eel (Anguilla japonica).

Marine biotechnology (New York, N.Y.)

Mitsuo, Itakura, Sato

Affiliations

  1. Faculty of Fisheries, Kagoshima University, 4-50-20, Shimoarata, Kagoshima 890-0056, Japan

PMID: 10489411 DOI: 10.1007/pl00011786

Abstract

: Cytochrome P450 1A (CYP1A) complementary DNA was isolated from eel (Anguilla japonica) liver treated with 3-methylcholanthrene. The cDNA contained a 5' untranslated region of 163 bp, an open reading flame of 1560 bp coding for 519 amino acids and a stop codon, and a 3' untranslated region of 1730 bp. The predicted molecular weight was approximately 58.4 kDa. The deduced amino acid sequence exhibited identities with reported CYP1A sequences of 80% for rainbow trout, 79% for scup, 76% for plaice and butterfly fish, and 74% for toadfish. When compared with mammalian CYP proteins, the eel CYP1A was more similar to CYP1A1 (54%-56%) than to CYP1A2 (49%-52%). Northern and Southern blot analyses showed two distinct bands, suggesting the existence of another 3-methylcholanthrene-inducible CYP1A gene in eel.

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