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Microb Ecol. 1999 Aug;38(2):126-135. doi: 10.1007/s002489900166.

Bacterioplankton Community Diversity in a Series of Thermally Stratified Lakes.

Microbial ecology

Konopka, Bercot, Nakatsu

Affiliations

  1. Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA

PMID: 10441705 DOI: 10.1007/s002489900166

Abstract

> Abstract The dominant members of the bacterioplankton community in a set of 10 small, thermally stratified lakes in northeastern Indiana were determined by denaturing gradient gel electrophoresis (DGGE) of a polymerase chain reaction amplified fragment of 16S rDNA. The variability in community composition was analyzed as function of vertical stratification (epilimnion vs metalimnion), time (July vs August samples), and geographical location. In 58 discrete samples, a range of 8-23 bands were detected (mean = 14, s.d. = 4). For all variables, sample pairs shared about 40-70% of bands. In comparisons between depth strata, pairs of oxic samples shared more bands than an oxic-anoxic pair. There was no obvious relationship between the geographical location of lakes (or their physical connection) and band sharing.http://link.springer-ny.com/link/service/journals/00248/bibs/38n2p126.html

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