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Houmiel KL, Slater S, Broyles D, et al. Poly(beta-hydroxybutyrate) production in oilseed leukoplasts of brassica napus. Planta. 1999;209(4):547-50doi: 10.1007/s004250050760.
Houmiel, K. L., Slater, S., Broyles, D., Casagrande, L., Colburn, S., Gonzalez, K., Mitsky, T. A., Reiser, S. E., Shah, D., Taylor, N. B., Tran, M., Valentin, H. E., & Gruys, K. J. (1999). Poly(beta-hydroxybutyrate) production in oilseed leukoplasts of brassica napus. Planta, 209(4), 547-50. https://doi.org/10.1007/s004250050760
Houmiel, et al. "Poly(beta-hydroxybutyrate) production in oilseed leukoplasts of brassica napus." Planta vol. 209,4 (1999): 547-50. doi: https://doi.org/10.1007/s004250050760
Houmiel KL, Slater S, Broyles D, Casagrande L, Colburn S, Gonzalez K, Mitsky TA, Reiser SE, Shah D, Taylor NB, Tran M, Valentin HE, Gruys KJ. Poly(beta-hydroxybutyrate) production in oilseed leukoplasts of brassica napus. Planta. 1999 Oct;209(4):547-50. doi: 10.1007/s004250050760. PMID: 10550638.
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Planta. 1999 Oct;209(4):547-50. doi: 10.1007/s004250050760.

Poly(beta-hydroxybutyrate) production in oilseed leukoplasts of brassica napus.

Planta

Houmiel, Slater, Broyles, Casagrande, Colburn, Gonzalez, Mitsky, Reiser, Shah, Taylor, Tran, Valentin, Gruys

Affiliations

  1. Monsanto Company, Agricultural Sector, 700 Chesterfield Parkway North, St. Louis, MO 63198, USA.

PMID: 10550638 DOI: 10.1007/s004250050760

Abstract

Polyhydroxyalkanoates (PHAs) comprise a class of biodegradable polymers which offer an environmentally sustainable alternative to petroleum-based plastics. Production of PHAs in plants is attractive since current fermentation technology is prohibitively expensive. The PHA homopolymer poly(beta-hydroxybutyrate) (PHB) has previously been produced in leaves of Arabidopsis thaliana (Nawrath et al., 1994, Proc Natl Acad Sci USA 91: 12760-12764). However, Brassica napus oilseed may provide a better system for PHB production because acetyl-CoA, the substrate required in the first step of PHB biosynthesis, is prevalent during fatty acid biosynthesis. Three enzymatic activities are needed to synthesize PHB: a beta-ketothiolase, an acetoacetyl-CoA reductase and a PHB synthase. Genes from the bacterium Ralstonia eutropha encoding these enzymes were independently engineered behind the seed-specific Lesquerella fendleri oleate 12-hydroxylase promoter in a modular fashion. The gene cassettes were sequentially transferred into a single, multi-gene vector which was used to transform B. napus. Poly(beta-hydroxybutyrate) accumulated in leukoplasts to levels as high as 7.7% fresh seed weight of mature seeds. Electron-microscopy analyses indicated that leukoplasts from these plants were distorted, yet intact, and appeared to expand in response to polymer accumulation.

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