J Magn Reson. 1999 Nov;141(1):180-4. doi: 10.1006/jmre.1999.1891.

Transverse-relaxation-optimized (TROSY) gradient-enhanced triple-resonance NMR spectroscopy.

Journal of magnetic resonance (San Diego, Calif. : 1997)

J P Loria, M Rance, A G Palmer

PMID: 10527755 DOI: 10.1006/jmre.1999.1891

Abstract

Two modifications to sensitivity-enhanced gradient-selected TROSY-based triple-resonance NMR experiments are proposed that reduce the overall duration of the pulse sequences and minimize radiation damping effects on water-flipback solvent suppression. The modifications are illustrated for the HNCO-TROSY experiment, but are applicable to all triple-resonance experiments that detect proton magnetization after a reverse polarization transfer step from a (15)N spin. The methods are applied to yeast triosephosphate isomerase, a symmetric dimer with 248 amino acid residues per monomer.

Copyright 1999 Academic Press.

MeSH terms

• Magnetic Resonance Spectroscopy / methods

Publication Types

• Journal Article
• Research Support, U.S. Gov't, Non-P.H.S.
• Research Support, U.S. Gov't, P.H.S.

Grant support

• GM19247/GM/NIGMS NIH HHS/United States
• GM40089/GM/NIGMS NIH HHS/United States
• GM59273/GM/NIGMS NIH HHS/United States