Display options
Cite
Bobe G, Beitz DC, Freeman AE, et al. Separation and Quantification of Bovine Milk Proteins by Reversed-Phase High-Performance Liquid Chromatography. J Agric Food Chem. 1998;46(2):458-463doi: 10.1021/jf970499p.
Bobe, G., Beitz, D. C., Freeman, A. E., & Lindberg, G. L. (1998). Separation and Quantification of Bovine Milk Proteins by Reversed-Phase High-Performance Liquid Chromatography. Journal of agricultural and food chemistry, 46(2), 458-463. https://doi.org/10.1021/jf970499p
Bobe, et al. "Separation and Quantification of Bovine Milk Proteins by Reversed-Phase High-Performance Liquid Chromatography." Journal of agricultural and food chemistry vol. 46,2 (1998): 458-463. doi: https://doi.org/10.1021/jf970499p
Bobe G, Beitz DC, Freeman AE, Lindberg GL. Separation and Quantification of Bovine Milk Proteins by Reversed-Phase High-Performance Liquid Chromatography. J Agric Food Chem. 1998 Feb 16;46(2):458-463. doi: 10.1021/jf970499p. PMID: 10554263.
Copy Download .nbib Format: NLM
Share it on

J Agric Food Chem. 1998 Feb 16;46(2):458-463. doi: 10.1021/jf970499p.

Separation and Quantification of Bovine Milk Proteins by Reversed-Phase High-Performance Liquid Chromatography.

Journal of agricultural and food chemistry

Bobe, Beitz, Freeman, Lindberg

Affiliations

  1. Department of Animal Science, Iowa State University, Ames, Iowa 50011.

PMID: 10554263 DOI: 10.1021/jf970499p

Abstract

Current analytical methods for milk proteins lack the capacity to simultaneously separate and quantify the six major bovine milk proteins and their genetic variants. A method is described that simultaneously separates and quantifies the six major bovine milk proteins. The separation is based on reversed-phase partitioning of the six major milk proteins and several genetic variants of kappa-casein, beta-casein, and beta-lactoglobulin. The described method has for each of the six milk proteins a linear quantitative response, precision (coefficient of variation below 5.1% within days of analysis, and below 7.1% between days of analysis), resolution (over 2.5 between proteins), peak efficiency (theoretical plate numbers between 8 000 and 50 000), and a sample treatment without filtration steps that together with the analysis takes 2 h. The composition of protein from milk of each of 234 cows was determined using the current method, and the results were similar to reference values for milk proteins.

Publication Types