FEMS Microbiol Ecol. 2000 Jan 01;31(1):21-28. doi: 10.1111/j.1574-6941.2000.tb00667.x.
FEMS microbiology ecology
Parham, Gibson
PMID: 10620715 DOI: 10.1111/j.1574-6941.2000.tb00667.x
Nitrate-limited batch cultures, incorporating 20 different fermentation substrates and inoculated with human faeces, mainly selected for the growth of enterobacteria. The microbial diversity involved was determined by a combination of phenotypic and genotypic procedures. Continuous culture with lactate as the sole electron donor selected for similar micro-organisms, but when antibiotics were incorporated to inhibit Escherichia coli and lactate was replaced with choline, there was a wider microbial diversity recovered. Clostridium ramosum and Bacteroides vulgatus were then isolated as well as enterobacteriaceae.