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Muttray AF, Mohn WW. Quantitation of the Population Size and Metabolic Activity of a Resin Acid Degrading Bacterium in Activated Sludge Using Slot-Blot Hybridization to Measure the rRNA:rDNA Ratio. Microb Ecol. 1999;38(4):348-357doi: 10.1007/s002489901005.
Muttray, A. F., & Mohn, W. W. (1999). Quantitation of the Population Size and Metabolic Activity of a Resin Acid Degrading Bacterium in Activated Sludge Using Slot-Blot Hybridization to Measure the rRNA:rDNA Ratio. Microbial ecology, 38(4), 348-357. https://doi.org/10.1007/s002489901005
Muttray, and Mohn. "Quantitation of the Population Size and Metabolic Activity of a Resin Acid Degrading Bacterium in Activated Sludge Using Slot-Blot Hybridization to Measure the rRNA:rDNA Ratio." Microbial ecology vol. 38,4 (1999): 348-357. doi: https://doi.org/10.1007/s002489901005
Muttray AF, Mohn WW. Quantitation of the Population Size and Metabolic Activity of a Resin Acid Degrading Bacterium in Activated Sludge Using Slot-Blot Hybridization to Measure the rRNA:rDNA Ratio. Microb Ecol. 1999 Nov;38(4):348-357. doi: 10.1007/s002489901005. PMID: 10758181.
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Microb Ecol. 1999 Nov;38(4):348-357. doi: 10.1007/s002489901005.

Quantitation of the Population Size and Metabolic Activity of a Resin Acid Degrading Bacterium in Activated Sludge Using Slot-Blot Hybridization to Measure the rRNA:rDNA Ratio.

Microbial ecology

Muttray, Mohn

Affiliations

  1. Department of Microbiology and Immunology, and Pulp and Paper Centre, University of British Columbia, #300-6174 University Boulevard, Vancouver, British Columbia, V6T 1Z3, Canada

PMID: 10758181 DOI: 10.1007/s002489901005

Abstract

The 16S rRNA:rDNA ratio is a useful parameter for measuring metabolic activity of a selected member of a complex microbial community, as in pulp effluent activated sludge systems. The RNA:DNA ratio of Sphingomonas sp. DhA-33, previously isolated from a sequencing batch reactor treating pulp mill effluent, is positively correlated with its growth rate (µ) under steady-state conditions. DhA-33 was grown in a chemostat with growth rates ranging from 0.04 to 0.15 cell divisions per hour. DhA-33 was also able to degrade dehydroabietic acid in bleached kraft mill effluent (BKME) plus mineral medium in batch culture. Slot-blot hybridization with radioactively labeled species-specific oligonucleotide probes for 16S rRNA and 16S rDNA was used to measure rRNA, rDNA, and the RNA:DNA ratio of this strain when in a mixed sludge community. An increase in DhA-33 rDNA indicated growth of DhA-33 within the community. The RNA:DNA ratio of DhA-33 increased sharply during exponential growth and declined as cells entered stationary phase. The RNA:DNA ratio decreased earlier and faster in DhA- 33/sludge co-cultures than in DhA-33 pure cultures, presumably due to an earlier depletion of nutrients. The species-specific quantification of the RNA:DNA ratio makes it possible to estimate the metabolic activity of selected members of a microbial community in situ.

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