Display options
Cite
Klein HH, Pich S, Lindert-Heimberg S, et al. Ultrastructural Evaluation of Postischemic Cell Death (Lethal Reperfusion Injury) in Porcine Hearts. J Thromb Thrombolysis. 1996;3(4):361-366doi: 10.1007/BF00133079.
Klein, H. H., Pich, S., Lindert-Heimberg, S., Nebendahl, K., Sprengel, U., Müller, H. H., & Schaper, J. (1996). Ultrastructural Evaluation of Postischemic Cell Death (Lethal Reperfusion Injury) in Porcine Hearts. Journal of thrombosis and thrombolysis, 3(4), 361-366. https://doi.org/10.1007/BF00133079
Klein, et al. "Ultrastructural Evaluation of Postischemic Cell Death (Lethal Reperfusion Injury) in Porcine Hearts." Journal of thrombosis and thrombolysis vol. 3,4 (1996): 361-366. doi: https://doi.org/10.1007/BF00133079
Klein HH, Pich S, Lindert-Heimberg S, Nebendahl K, Sprengel U, Müller HH, Schaper J. Ultrastructural Evaluation of Postischemic Cell Death (Lethal Reperfusion Injury) in Porcine Hearts. J Thromb Thrombolysis. 1996;3(4):361-366. doi: 10.1007/BF00133079. PMID: 10602565.
Copy Download .nbib Format: NLM
Share it on

J Thromb Thrombolysis. 1996;3(4):361-366. doi: 10.1007/BF00133079.

Ultrastructural Evaluation of Postischemic Cell Death (Lethal Reperfusion Injury) in Porcine Hearts.

Journal of thrombosis and thrombolysis

Klein, Pich, Lindert-Heimberg, Nebendahl, Sprengel, Müller, Schaper

Affiliations

  1. Department of Cardiology, University of Marburg, FRG and Abteilung für Kardiologie, Städt. Krankenanstalten Idar-Oberstein GmbH, Dr. Ottmar-Kohler-Str. 2, 55743 Idar-Oberstein, FRG.

PMID: 10602565 DOI: 10.1007/BF00133079

Abstract

This study investigated whether reperfusion results in an increase of ultrastructurally determined myocardial injury in pig hearts. The left anterior descending coronary artery (LAD) was distally occluded in 12 pigs for 35-45 minutes and then reperfused for 3 hours. At the end of ischemia, as well as after 3 hours of reperfusion, one transmural biopsy was removed from the center of the risk region and subdivided into four-specimens, representing the subendocardial (I), subendo-midmyocardial (II), subepi-midmyocardial (III), and subepicardial layers (IV). The degree of injury was assessed by electronmicroscopy and was scored as reversible (1), an almost equal mixture of reversible and irreversible (2), and totally irreversible (3) damage. In addition, infarct size was determined as the ratio of infarcted (tetrazolium stain) to ischemic (dye technique) myocardium. Infarct sizes ranged from 29.3% to 93% (mean 61.2%). The scores of injury of the four tissue layers before and after reperfusion did not differ significantly: layer I, 2.4 +/- 0.8/2.3 +/- 0.9; layer II, 2.2 +/- 0.9/2.0 +/- 0.9; layer III, 1.8 +/- 0.9/2.0 +/- 0.9; and layer IV, 1.6 +/- 0.9/1.3 +/- 0.6. The means of the four layers were almost identical at the end of ischemia (2.1 +/- 0.8) and after 3 hours of reperfusion (2.0 +/- 0.6). A linear regression analysis with 95% confidence limits of the score values before and after reperfusion indicated that maximally 25% of a mean final infarct size of about 50% may be due to lethal reperfusion injury. This study suggests that cell death in regional ischemia and reperfusion occurs predominantly during ischemia and not during reperfusion.

References

  1. Circ Res. 1954 Sep;2(5):460-5 - PubMed
  2. Eur Heart J. 1986 Jul;7 Suppl B:3-9 - PubMed
  3. Circulation. 1989 Oct;80(4):1049-62 - PubMed
  4. Clin Cardiol. 1979 Oct;2(5):317-27 - PubMed
  5. Circ Res. 1982 Dec;51(6):683-93 - PubMed
  6. Basic Res Cardiol. 1984 Mar-Apr;79(2):164-75 - PubMed
  7. Am J Cardiovasc Pathol. 1993;4(4):336-42 - PubMed
  8. Circulation. 1986 Dec;74(6):1424-33 - PubMed
  9. J Am Coll Cardiol. 1993 Feb;21(2):537-45 - PubMed
  10. Lab Invest. 1979 Jun;40(6):633-44 - PubMed
  11. Circulation. 1987 May;75(5):1074-82 - PubMed
  12. J Mol Cell Cardiol. 1979 Jun;11(6):521-41 - PubMed
  13. Arch Pathol. 1965 Feb;79:135-43 - PubMed
  14. Cardiovasc Res. 1993 Jan;27(1):9-17 - PubMed
  15. Circulation. 1992 Mar;85(3):1160-74 - PubMed
  16. J Mol Cell Cardiol. 1984 Jan;16(1):79-94 - PubMed
  17. Basic Res Cardiol. 1988 Sep-Oct;83(5):550-9 - PubMed
  18. J Am Coll Cardiol. 1989 May;13(6):1395-401 - PubMed
  19. Basic Res Cardiol. 1988 Mar-Apr;83(2):141-8 - PubMed
  20. Circulation. 1990 Sep;82(3):1020-33 - PubMed

Publication Types