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Laberge D, Rouillon R, Carpentier R. Comparative study of thylakoid membranes sensitivity for herbicide detection after physical or chemical immobilization. Enzyme Microb Technol. 2000;26(5):332-336doi: 10.1016/s0141-0229(99)00174-x.
Laberge, D., Rouillon, R., & Carpentier, R. (2000). Comparative study of thylakoid membranes sensitivity for herbicide detection after physical or chemical immobilization. Enzyme and microbial technology, 26(5), 332-336. https://doi.org/10.1016/s0141-0229(99)00174-x
Laberge, et al. "Comparative study of thylakoid membranes sensitivity for herbicide detection after physical or chemical immobilization." Enzyme and microbial technology vol. 26,5 (2000): 332-336. doi: https://doi.org/10.1016/s0141-0229(99)00174-x
Laberge D, Rouillon R, Carpentier R. Comparative study of thylakoid membranes sensitivity for herbicide detection after physical or chemical immobilization. Enzyme Microb Technol. 2000 Mar 01;26(5):332-336. doi: 10.1016/s0141-0229(99)00174-x. PMID: 10713204.
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Enzyme Microb Technol. 2000 Mar 01;26(5):332-336. doi: 10.1016/s0141-0229(99)00174-x.

Comparative study of thylakoid membranes sensitivity for herbicide detection after physical or chemical immobilization.

Enzyme and microbial technology

Laberge, Rouillon, Carpentier

Affiliations

  1. Groupe de Recherche en Énergie et Information Biomoléculaires, Université du Québec à Trois-Rivières, C.P. 500, Trois-Rivières (Québec), Canada

PMID: 10713204 DOI: 10.1016/s0141-0229(99)00174-x

Abstract

A micro-test using immobilized thylakoid membranes as sensing element in a micro-electrochemical cell has been developed to assess impairment at the level of the light-driven transport of electrons. In this study, thylakoids isolated from spinach leaves were either immobilized by entrapment in poly(vinylalcohol) bearing styrylpyridinium groups or by chemical immobilization in an albumin-glutaraldehyde crosslinked matrix. The two immobilization procedures were compared upon the sensitivity of the immobilized materials to detect nine herbicides targetting photosystem II. Despite the largely differing mode of immobilization, the procedures led to strikingly similar detection capabilities for herbicides. Inherent characteristics of both immobilization procedures are also discussed.

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