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Miyakawa H, Kitazawa E, Fujikawa H, et al. Analysis of two major anti-M2 antibodies (anti-PDC-E2/anti-BCOADC-E2) in primary biliary cirrhosis: relationship to titers of immunofluorescent anti-mitochondrial antibody. Hepatol Res. 2000;18(1):1-9doi: 10.1016/s1386-6346(99)00079-0.
Miyakawa, H., Kitazawa, E., Fujikawa, H., Kikuchi, K., Abe, K., Kawaguchi, N., & Kako, M. (2000). Analysis of two major anti-M2 antibodies (anti-PDC-E2/anti-BCOADC-E2) in primary biliary cirrhosis: relationship to titers of immunofluorescent anti-mitochondrial antibody. Hepatology research : the official journal of the Japan Society of Hepatology, 18(1), 1-9. https://doi.org/10.1016/s1386-6346(99)00079-0
Miyakawa, et al. "Analysis of two major anti-M2 antibodies (anti-PDC-E2/anti-BCOADC-E2) in primary biliary cirrhosis: relationship to titers of immunofluorescent anti-mitochondrial antibody." Hepatology research : the official journal of the Japan Society of Hepatology vol. 18,1 (2000): 1-9. doi: https://doi.org/10.1016/s1386-6346(99)00079-0
Miyakawa H, Kitazawa E, Fujikawa H, Kikuchi K, Abe K, Kawaguchi N, Kako M. Analysis of two major anti-M2 antibodies (anti-PDC-E2/anti-BCOADC-E2) in primary biliary cirrhosis: relationship to titers of immunofluorescent anti-mitochondrial antibody. Hepatol Res. 2000 Jul;18(1):1-9. doi: 10.1016/s1386-6346(99)00079-0. PMID: 10838031.
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Hepatol Res. 2000 Jul;18(1):1-9. doi: 10.1016/s1386-6346(99)00079-0.

Analysis of two major anti-M2 antibodies (anti-PDC-E2/anti-BCOADC-E2) in primary biliary cirrhosis: relationship to titers of immunofluorescent anti-mitochondrial antibody.

Hepatology research : the official journal of the Japan Society of Hepatology

Miyakawa, Kitazawa, Fujikawa, Kikuchi, Abe, Kawaguchi, Kako

Affiliations

  1. Fourth Department of Internal Medicine, Teikyo University School of Medicine, 3-8-3 Mizonokuchi, Takatsu-ku, Kawasaki-shi, 213-8507, Kanagawa, Japan

PMID: 10838031 DOI: 10.1016/s1386-6346(99)00079-0

Abstract

To analyze anti-M2 components in primary biliary cirrhosis (PBC) we measured two major anti-M2 antibodies (anti-PDC-E2 and anti-BCOADC-E2) by immunoblotting and ELISA, and compared the results between 38 immunofluorescent anti-mitochondrial antibody (AMA)-negative PBC patients (group A) and 39 strongly AMA-positive PBC patients (group B) with titers of 1:640. Using bovine heart mitochondrial fraction as antigen, the immunoblot positivity rate of anti-PDC-E2 in group B was significantly higher than that in group A, whereas the positivity rate of anti-BCOADC-E2 was not significantly different between the two groups. This result was similar to that obtained by ELISA using recombinant fusion proteins. In group A there was a significant inverse correlation between ELISA optical density values of anti-PDC-E2 and of anti-BCOADC-E2, but in group B there was no correlation between the two values. Only three patients from group A and 21 from group B were positive for both antibodies. Taken together these results appear to indicate that the detection of anti-BCOADC-E2 is critical for the accurate serological diagnosis of AMA-negative PBC patients. The detection of anti-BCOADC-E2 may also help to distinguish between AMA-negative PBC and autoimmune cholangitis patients.

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