Display options
Cite
Willeman WF, Hanefeld U, Straathof AJ, et al. Estimation of kinetic parameters by progress curve analysis for the synthesis of (R)-mandelonitrile by Prunus amygdalus hydroxynitrile lyase. Enzyme Microb Technol. 2000;27(6):423-433doi: 10.1016/s0141-0229(00)00226-x.
Willeman, W. F., Hanefeld, U., Straathof, A. J., & Heijnen, J. J. (2000). Estimation of kinetic parameters by progress curve analysis for the synthesis of (R)-mandelonitrile by Prunus amygdalus hydroxynitrile lyase. Enzyme and microbial technology, 27(6), 423-433. https://doi.org/10.1016/s0141-0229(00)00226-x
Willeman, et al. "Estimation of kinetic parameters by progress curve analysis for the synthesis of (R)-mandelonitrile by Prunus amygdalus hydroxynitrile lyase." Enzyme and microbial technology vol. 27,6 (2000): 423-433. doi: https://doi.org/10.1016/s0141-0229(00)00226-x
Willeman WF, Hanefeld U, Straathof AJ, Heijnen JJ. Estimation of kinetic parameters by progress curve analysis for the synthesis of (R)-mandelonitrile by Prunus amygdalus hydroxynitrile lyase. Enzyme Microb Technol. 2000 Sep 01;27(6):423-433. doi: 10.1016/s0141-0229(00)00226-x. PMID: 10938422.
Copy Download .nbib Format: NLM
Share it on

Enzyme Microb Technol. 2000 Sep 01;27(6):423-433. doi: 10.1016/s0141-0229(00)00226-x.

Estimation of kinetic parameters by progress curve analysis for the synthesis of (R)-mandelonitrile by Prunus amygdalus hydroxynitrile lyase.

Enzyme and microbial technology

Willeman, Hanefeld, Straathof, Heijnen

Affiliations

  1. Delft University of Technology, Kluyver Laboratory for Biotechnology Julianalaan 67, NL-2628, BC Delft, The Netherlands

PMID: 10938422 DOI: 10.1016/s0141-0229(00)00226-x

Abstract

Consistent sets of kinetic parameters were estimated for the synthesis of (R)-mandelonitrile, catalyzed by Prunus amygdalus hydroxynitrile lyase, at 5 and 25 degrees C and pH 5.5 by progress curve analysis. The rate constants and equilibrium constants of the nonenzymatic reaction were determined separately to reduce the number of parameters to be estimated simultaneously. At a lower temperature the equilibrium is much more favorable and the formation of rac-mandelonitrile by the nonenzymatic reaction is suppressed. The estimated kinetic parameters were used to identify that the rate determining step in the catalytic cycle is the release of (R)-mandelonitrile from the ternary complex.

Publication Types