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Romeis T, Kämper J, Kahmann R. Single-chain fusions of two unrelated homeodomain proteins trigger pathogenicity in Ustilago maydis. Proc Natl Acad Sci U S A. 1997;94(4):1230-4doi: 10.1073/pnas.94.4.1230.
Romeis, T., Kämper, J., & Kahmann, R. (1997). Single-chain fusions of two unrelated homeodomain proteins trigger pathogenicity in Ustilago maydis. Proceedings of the National Academy of Sciences of the United States of America, 94(4), 1230-4. https://doi.org/10.1073/pnas.94.4.1230
Romeis, T, et al. "Single-chain fusions of two unrelated homeodomain proteins trigger pathogenicity in Ustilago maydis." Proceedings of the National Academy of Sciences of the United States of America vol. 94,4 (1997): 1230-4. doi: https://doi.org/10.1073/pnas.94.4.1230
Romeis T, Kämper J, Kahmann R. Single-chain fusions of two unrelated homeodomain proteins trigger pathogenicity in Ustilago maydis. Proc Natl Acad Sci U S A. 1997 Feb 18;94(4):1230-4. doi: 10.1073/pnas.94.4.1230. PMID: 11038599; PMCID: PMC19773.
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Proc Natl Acad Sci U S A. 1997 Feb 18;94(4):1230-4. doi: 10.1073/pnas.94.4.1230.

Single-chain fusions of two unrelated homeodomain proteins trigger pathogenicity in Ustilago maydis.

Proceedings of the National Academy of Sciences of the United States of America

T Romeis, J Kämper, R Kahmann

Affiliations

  1. Institut für Genetik und Mikrobiologie der Universität München, Maria-Ward-Strasse 1a, 80638 Munich, Germany.

PMID: 11038599 PMCID: PMC19773 DOI: 10.1073/pnas.94.4.1230

Abstract

Pathogenic and sexual development of the fungus Ustilago maydis, the causal agent of corn smut disease, is regulated by heterodimerization of two unrelated homeodomain proteins bE and bW, both encoded by the multi-allelic b mating-type locus. This complex can only be formed if the two proteins are derived from different alleles. The heterodimer is believed to function as a transcriptional regulator that binds to target sites upstream of developmentally regulated genes. We have synthesized a translational fusion in which bE is tethered to bW by a designed flexible kink region. U. maydis strains expressing this synthetic b-fusion become pathogenic for corn illustrating that the single-chain fusion substitutes for the active bE/bW heterodimer. Synthetic b-fusions in which bE and bW originate from the same allele as well as fusions deleted for the dimerization domains were shown to be active while both homeodomains were required for function. Such active fusion proteins are expected to be instrumental in the identification of pathogenicity genes.

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