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Tomme P, Boraston A, Kormos JM, et al. Affinity electrophoresis for the identification and characterization of soluble sugar binding by carbohydrate-binding modules. Enzyme Microb Technol. 2000;27(7):453-458doi: 10.1016/s0141-0229(00)00246-5.
Tomme, P., Boraston, A., Kormos, J. M., Warren, R. A., & Kilburn, D. G. (2000). Affinity electrophoresis for the identification and characterization of soluble sugar binding by carbohydrate-binding modules. Enzyme and microbial technology, 27(7), 453-458. https://doi.org/10.1016/s0141-0229(00)00246-5
Tomme, et al. "Affinity electrophoresis for the identification and characterization of soluble sugar binding by carbohydrate-binding modules." Enzyme and microbial technology vol. 27,7 (2000): 453-458. doi: https://doi.org/10.1016/s0141-0229(00)00246-5
Tomme P, Boraston A, Kormos JM, Warren RA, Kilburn DG. Affinity electrophoresis for the identification and characterization of soluble sugar binding by carbohydrate-binding modules. Enzyme Microb Technol. 2000 Oct 01;27(7):453-458. doi: 10.1016/s0141-0229(00)00246-5. PMID: 10978766.
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Enzyme Microb Technol. 2000 Oct 01;27(7):453-458. doi: 10.1016/s0141-0229(00)00246-5.

Affinity electrophoresis for the identification and characterization of soluble sugar binding by carbohydrate-binding modules.

Enzyme and microbial technology

Tomme, Boraston, Kormos, Warren, Kilburn

Affiliations

  1. Protein Engineering Network of Centres of Excellence, Department of Microbiology and Immunology and The Biotechnology Laboratory, #237-6174 University of British Columbia, V6T 1Z3, Vancouver, BC, Canada

PMID: 10978766 DOI: 10.1016/s0141-0229(00)00246-5

Abstract

Affinity electrophoresis was used to identify and quantify the interaction of carbohydrate-binding modules (CBMs) with soluble polysaccharides. Association constants determined by AE were in excellent agreement with values obtained by isothermal titration calorimetry and fluorescence titration. The method was adapted to the identification, study and characterization of mutant carbohydrate-binding modules with altered affinities and specificities. Competition affinity electrophoresis was used to monitor binding of small, soluble mono- and disaccharides to one of the modules.

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