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Yamashita M, Iwabuchi K. Bombyx mori prohemocyte division and differentiation in individual microcultures. J Insect Physiol. 2001;47(4):325-31doi: 10.1016/s0022-1910(00)00144-x.
Yamashita, M., & Iwabuchi, K. (2001). Bombyx mori prohemocyte division and differentiation in individual microcultures. Journal of insect physiology, 47(4), 325-31. https://doi.org/10.1016/s0022-1910(00)00144-x
Yamashita, M, and Iwabuchi, K. "Bombyx mori prohemocyte division and differentiation in individual microcultures." Journal of insect physiology vol. 47,4 (2001): 325-31. doi: https://doi.org/10.1016/s0022-1910(00)00144-x
Yamashita M, Iwabuchi K. Bombyx mori prohemocyte division and differentiation in individual microcultures. J Insect Physiol. 2001 Apr;47(4):325-31. doi: 10.1016/s0022-1910(00)00144-x. PMID: 11166296.
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J Insect Physiol. 2001 Apr;47(4):325-31. doi: 10.1016/s0022-1910(00)00144-x.

Bombyx mori prohemocyte division and differentiation in individual microcultures.

Journal of insect physiology

M Yamashita, K Iwabuchi

Affiliations

  1. Tokyo University of Agriculture and Technology, Saiwai-cho, Fuchu, 183-8509, Tokyo, Japan

PMID: 11166296 DOI: 10.1016/s0022-1910(00)00144-x

Abstract

We followed the fate of microcultured Bombyx mori prohemocytes in vitro. Prohemocytes isolated from larval hemolymph (day 1 of 4th instar) were maintained for 4-11 days in serum-free MGM-450 medium and some of them underwent mitotic division. Over 60% of the non-dividing prohemocytes differentiated to plasmatocytes or granulocytes. Some of the granulocytes subsequently transformed to spherulocytes. Of the dividing prohemocytes, 59.2% of the daughter cells differentiated into other types of hemocytes such as plasmatocytes, granulocytes and spherulocytes, and the remainder divided into new prohemocytes. Four of these renewed prohemocytes generated daughter cells composed of plasmatocytes and granulocytes. These results suggest that prohemocytes possess the properties of stem cells, and that plasmatocytes and spherulocytes may be terminally differentiated cells, whereas granulocytes, at least in part, may be a transient form of spherulocyte. Oenocytoids were not produced, suggesting that the lineage of oenocytoids differs from that of other types of hemocytes and that it is determined before release from hemopoietic organs.

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