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Starchev K, Ricka J, Buffle J. Noise on Fluorescence Correlation Spectroscopy. J Colloid Interface Sci. 2001;233(1):50-55doi: 10.1006/jcis.2000.7229.
Starchev, K., Ricka, J., & Buffle, J. (2001). Noise on Fluorescence Correlation Spectroscopy. Journal of colloid and interface science, 233(1), 50-55. https://doi.org/10.1006/jcis.2000.7229
Starchev, et al. "Noise on Fluorescence Correlation Spectroscopy." Journal of colloid and interface science vol. 233,1 (2001): 50-55. doi: https://doi.org/10.1006/jcis.2000.7229
Starchev K, Ricka J, Buffle J. Noise on Fluorescence Correlation Spectroscopy. J Colloid Interface Sci. 2001 Jan 01;233(1):50-55. doi: 10.1006/jcis.2000.7229. PMID: 11112305.
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J Colloid Interface Sci. 2001 Jan 01;233(1):50-55. doi: 10.1006/jcis.2000.7229.

Noise on Fluorescence Correlation Spectroscopy.

Journal of colloid and interface science

Starchev, Ricka, Buffle

Affiliations

  1. Department of Inorganic, Analytical, and Applied Chemistry, University of Geneva, Sciences II, 30 Quai Ernest Ansermet, Geneva 4, 1211, Switzerland

PMID: 11112305 DOI: 10.1006/jcis.2000.7229

Abstract

The time dependence of the noise and the signal-to-noise (SN) ratio of the fluorescence correlation spectroscopy (FCS) autocorrelation function is obtained from replica measurements of standard dextran solutions. The noise dependence on the delay time is fitted by a hyperbolic function with two fitting parameters. The dependence of these parameters on concentration, fluorescence intensity, and accumulation time is obtained experimentally. The behavior of SN at zero delay time agrees well with the theoretical predictions reported in the literature. The obtained data are useful for the quantitative evaluation of the FCS data fits, as well as for simulation of the FCS autocorrelation functions. Copyright 2001 Academic Press.

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