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Kobori H, Hayashi M, Saruta T. Thyroid Hormone Stimulates Renin Gene Expression Through the Thyroid Hormone Response Element. Hypertension. 2001;37(1):99-104doi: 10.1161/01.hyp.37.1.99.
Kobori, H., Hayashi, M., & Saruta, T. (2001). Thyroid Hormone Stimulates Renin Gene Expression Through the Thyroid Hormone Response Element. Hypertension (Dallas, Tex. : 1979), 37(1), 99-104. https://doi.org/10.1161/01.hyp.37.1.99
Kobori, Hiroyuki, et al. "Thyroid Hormone Stimulates Renin Gene Expression Through the Thyroid Hormone Response Element." Hypertension (Dallas, Tex. : 1979) vol. 37,1 (2001): 99-104. doi: https://doi.org/10.1161/01.hyp.37.1.99
Kobori H, Hayashi M, Saruta T. Thyroid Hormone Stimulates Renin Gene Expression Through the Thyroid Hormone Response Element. Hypertension. 2001 Jan;37(1):99-104. doi: 10.1161/01.hyp.37.1.99. PMID: 11208763; PMCID: PMC2573046.
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Hypertension. 2001 Jan;37(1):99-104. doi: 10.1161/01.hyp.37.1.99.

Thyroid Hormone Stimulates Renin Gene Expression Through the Thyroid Hormone Response Element.

Hypertension (Dallas, Tex. : 1979)

Hiroyuki Kobori, Matsuhiko Hayashi, Takao Saruta

Affiliations

  1. Department of Internal Medicine, Keio University School of Medicine, Tokyo, Japan.

PMID: 11208763 PMCID: PMC2573046 DOI: 10.1161/01.hyp.37.1.99

Abstract

-We previously reported that thyroid hormone stimulates renin synthesis in vivo and in vitro. Here, we analyzed the 5'-flanking sequence of the human renin gene for promoter activity responsive to thyroid hormone using Calu-6 cells, which secrete renin endogenously and express thyroid hormone receptor-ss. The luciferase reporter gene was cloned together with 5'-flanking portions of the human renin gene of various lengths into the pGL3-Basic vector. Luciferase activity assays were performed using the Dual Luciferase Reporter Assay System. 3,3',5-Triiodo-L-thyronine stimulated the promoter activity of pGL3-Basic-1111/+12 and pGL3-Basic-1298/+12 by 2.3+/-0.1- and 1.7+/-0.1-fold, respectively. Shorter constructs (pGL3-Basic-144/+12, pGL3-Basic-226/+12, pGL3-Basic-452/+12, and pGL3-Basic-953/+12) were not stimulated by thyroid hormone. These results suggest that there is a possible thyroid hormone response element (5'-AGG TCA GGT CAc aat GTT CCT-3') between nucleotides -1111 and -953. In 3 constructs with site-directed mutations in this sequence, basal promoter activities were significantly increased, whereas promoter activation by thyroid hormone was abolished. Electrophoretic mobility shift assays showed that the -1111/-953 DNA fragment of the intact human renin gene was bound to nuclear proteins of Calu-6 cells; however, none of the 3 mutant probes were bound to any nuclear proteins. These results suggest that thyroid hormone stimulates the promoter activity of the human renin gene through thyroid hormone response element-dependent mechanisms in Calu-6 cells.

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