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Dekker RF, Barbosa AM. The effects of aeration and veratryl alcohol on the production of two laccases by the ascomycete Botryosphaeria sp. Enzyme Microb Technol. 2001;28(1):81-88doi: 10.1016/s0141-0229(00)00274-x.
Dekker, R. F., & Barbosa, A. M. (2001). The effects of aeration and veratryl alcohol on the production of two laccases by the ascomycete Botryosphaeria sp. Enzyme and microbial technology, 28(1), 81-88. https://doi.org/10.1016/s0141-0229(00)00274-x
Dekker, and Barbosa. "The effects of aeration and veratryl alcohol on the production of two laccases by the ascomycete Botryosphaeria sp." Enzyme and microbial technology vol. 28,1 (2001): 81-88. doi: https://doi.org/10.1016/s0141-0229(00)00274-x
Dekker RF, Barbosa AM. The effects of aeration and veratryl alcohol on the production of two laccases by the ascomycete Botryosphaeria sp. Enzyme Microb Technol. 2001 Jan 02;28(1):81-88. doi: 10.1016/s0141-0229(00)00274-x. PMID: 11118601.
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Enzyme Microb Technol. 2001 Jan 02;28(1):81-88. doi: 10.1016/s0141-0229(00)00274-x.

The effects of aeration and veratryl alcohol on the production of two laccases by the ascomycete Botryosphaeria sp.

Enzyme and microbial technology

Dekker, Barbosa

Affiliations

  1. Division of Science, Biotechnology Program, Murdoch University, 6150, W.A., Murdoch, W.A., Australia

PMID: 11118601 DOI: 10.1016/s0141-0229(00)00274-x

Abstract

The ascomycete, Botryosphaeria sp, produced two extracellular constitutive laccases (PPO-I and PPO-II) active toward the substrates: 2, 2(1)-azino-bis(3-ethyl-benzthiazoline-6-sulfonic acid) [ABTS], and 2,6-dimethoxyphenol (DMP), respectively. The production of both laccases increased when the fungal isolate was grown in the presence of veratryl alcohol, and resulted in optimal laccase production (100- and 25- fold, respectively) at 40 mM. The effect of aeration on growth and laccase production was studied in baffled flasks, and showed that aeration of the cultures increased the production of both enzymes 4-5 fold in the presence of veratryl alcohol. Both laccases were susceptible to inhibition by azide, acetate and chloride anions. Veratryl alcohol inhibited the laccase-catalyzed polymerization of DMP. Growing cultures of Botryosphaeria sp. produced an exopolysaccharide of the beta-glucan type whose synthesis was depressed when grown in the presence of veratryl alcohol.

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