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Ben Mouaz A, Lindheimer M, Montet JC, et al. A study of the adsorption of bile salts onto model lecithin membranes. Colloids Surf B Biointerfaces. 2001;20(2):119-127doi: 10.1016/s0927-7765(00)00185-5.
Ben Mouaz A, Lindheimer, M., Montet, J. C., Zajac, J., & Lagerge, S. (2001). A study of the adsorption of bile salts onto model lecithin membranes. Colloids and surfaces. B, Biointerfaces, 20(2), 119-127. https://doi.org/10.1016/s0927-7765(00)00185-5
Ben Mouaz A, et al. "A study of the adsorption of bile salts onto model lecithin membranes." Colloids and surfaces. B, Biointerfaces vol. 20,2 (2001): 119-127. doi: https://doi.org/10.1016/s0927-7765(00)00185-5
Ben Mouaz A, Lindheimer M, Montet JC, Zajac J, Lagerge S. A study of the adsorption of bile salts onto model lecithin membranes. Colloids Surf B Biointerfaces. 2001 Feb 01;20(2):119-127. doi: 10.1016/s0927-7765(00)00185-5. PMID: 11087984.
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Colloids Surf B Biointerfaces. 2001 Feb 01;20(2):119-127. doi: 10.1016/s0927-7765(00)00185-5.

A study of the adsorption of bile salts onto model lecithin membranes.

Colloids and surfaces. B, Biointerfaces

Ben Mouaz A, Lindheimer, Montet, Zajac, Lagerge

Affiliations

  1. Laboratoire des Agrégats Moléculaires et Matériaux Inorganiques, ESA 5072, Université Montpellier II, Place E. Bataillon CC 015, 34095 Cedex 05, Montpellier, France

PMID: 11087984 DOI: 10.1016/s0927-7765(00)00185-5

Abstract

Bile salts play a central role in the promotion of cytotoxicity or cytoprotection. In this study, we examined the interaction of different bile salts with egg lecithin vesicles using 31P NMR spectroscopy. The effects of taurochenodeoxycholate (TCDC or 3alpha,7alpha,-dihydroxy-5beta-cholanoyl taurine, of tauroursodeoxycholate (TUDC) or 3alpha,7beta,-dihydroxy-5beta-cholanoyl taurine) and of taurobetamuricholate (TbetaMC or 3alpha,6beta,7beta,-trihydroxy-5beta-cholanoyl taurine), at various bile salt/lecithin ratios, were evaluated. From the percent 31P present in vesicles, the micellar capacity of bile salts to dissolve lecithin was determined. TCDC was incorporated into vesicles for bile salt/lecithin molar ratios lower than 0.62 while for TUDC and TbetaMC, the critical ratios were 0.94 and 1.1, respectively. The 31P chemical shift change was markedly larger with TCDC than that found with TUDC and TbetaMC. In order to specify the low interactions observed between hydrophilic bile salts and lecithin, we determined the intermixed micellar/vesicular bile salt concentrations (IMVC) of bile salt/lecithin solutions using rapid ultrafiltration-centrifugation for TUDC and lecithin solubility measurements for TUDC, TbetaMC and TCDC. The low IMVC obtained indicate that even hydrophilic bile salts were bound mostly to the mixed aggregates. In conclusion, the low disturbance in the arrangement of lecithin induced by TUDC and TbetaMC appears to be due to the interfacial location of these bile salts. TCDC (7alpha OH) penetrates more deeply in the membrane than the 7beta hydroxylated bile salts that may partly explain the distinct damaging effects of these bile salts.

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