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Iwata H, Kobayashi S, Iwase H, et al. Flow Cytometric Assay of c-erbB-2 Protein in Fine Needle Aspirates of Fresh and Frozen Human Breast Cancer Tissues. Breast Cancer. 1996;3(2):111-117doi: 10.1007/BF02966971.
Iwata, H., Kobayashi, S., Iwase, H., Itoh, Y., Kuzushima, T., Naitoh, A., Yamashita, T., Itoh, K., Toyama, T., & Masaoka, A. (1996). Flow Cytometric Assay of c-erbB-2 Protein in Fine Needle Aspirates of Fresh and Frozen Human Breast Cancer Tissues. Breast cancer (Tokyo, Japan), 3(2), 111-117. https://doi.org/10.1007/BF02966971
Iwata, et al. "Flow Cytometric Assay of c-erbB-2 Protein in Fine Needle Aspirates of Fresh and Frozen Human Breast Cancer Tissues." Breast cancer (Tokyo, Japan) vol. 3,2 (1996): 111-117. doi: https://doi.org/10.1007/BF02966971
Iwata H, Kobayashi S, Iwase H, Itoh Y, Kuzushima T, Naitoh A, Yamashita T, Itoh K, Toyama T, Masaoka A. Flow Cytometric Assay of c-erbB-2 Protein in Fine Needle Aspirates of Fresh and Frozen Human Breast Cancer Tissues. Breast Cancer. 1996 Jun 28;3(2):111-117. doi: 10.1007/BF02966971. PMID: 11091562.
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Breast Cancer. 1996 Jun 28;3(2):111-117. doi: 10.1007/BF02966971.

Flow Cytometric Assay of c-erbB-2 Protein in Fine Needle Aspirates of Fresh and Frozen Human Breast Cancer Tissues.

Breast cancer (Tokyo, Japan)

Iwata, Kobayashi, Iwase, Itoh, Kuzushima, Naitoh, Yamashita, Itoh, Toyama, Masaoka

Affiliations

  1. The Second Department of Surgery, Nagoya City University Medical School, 1 Kawasumi, Mizuho-cho, Mizuho-ku, 467, Japan.

PMID: 11091562 DOI: 10.1007/BF02966971

Abstract

Using FCM (flow cytometry), we analyzed the ErbB-2 (c-erbB-2 protein) status of 61 breast cancer tissue samples obtained by FNAB (fine-needle aspiration biopsy). The number of cancer cells collected by FNAB from fresh samples was 1.7+/-0.7 x10&sup5;, 2.5 +/-0.6 x10&sup5;, and 4.0+/- 0.8x10 &sup5;, by single, double and triple aspirations, respectively. A mean of 3.0+/-0.6 x10&sup5;cells was collected on three aspirations from frozen samples. The number of cells collected on three aspirations was sufficient for the measurement of ErbB-2. Cells with higher ErbB-2 levels than those of normal human lymphocytes were designated ErbB-2 positive cells. The mean overall for ErbB-2 positive cell rates was 20.5 +/- 27.9 % (mean+/-SD). The rates were 27.9 +/- 31.6 % in patients with recurrence and 15.9 +/- 24.4% in patients without recurrence. When the cut-off value was set at 20% of the positive cell rate (P =0.008, generalized Wilcoxon test), patients with ErbB-2 negative tumors showed highly significantly longer survival without recurrence (P=0.008, generalized Wilcoxontest) and better overall survival rates (P=0.013) than patients with ErbB-2 positive tumors. Among 61 specimens, 16 (26.2%) scored positive for ErbB-2 by FCM. These finding indicated that the analysis of ErbB-2 status using FCM of samples obtained by FNAB should be useful for preoperatively evaluating the prognosis of patients with breast cancer.

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