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Meyer A, Raba C, Fischer K. Ion-pair RP-HPLC determination of sugars, amino sugars, and uronic acids after derivatization with p-aminobenzoic acid. Anal Chem. 2001;73(11):2377-82doi: 10.1021/ac001402s.
Meyer, A., Raba, C., & Fischer, K. (2001). Ion-pair RP-HPLC determination of sugars, amino sugars, and uronic acids after derivatization with p-aminobenzoic acid. Analytical chemistry, 73(11), 2377-82. https://doi.org/10.1021/ac001402s
Meyer, A, et al. "Ion-pair RP-HPLC determination of sugars, amino sugars, and uronic acids after derivatization with p-aminobenzoic acid." Analytical chemistry vol. 73,11 (2001): 2377-82. doi: https://doi.org/10.1021/ac001402s
Meyer A, Raba C, Fischer K. Ion-pair RP-HPLC determination of sugars, amino sugars, and uronic acids after derivatization with p-aminobenzoic acid. Anal Chem. 2001 Jun 01;73(11):2377-82. doi: 10.1021/ac001402s. PMID: 11403275.
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Anal Chem. 2001 Jun 01;73(11):2377-82. doi: 10.1021/ac001402s.

Ion-pair RP-HPLC determination of sugars, amino sugars, and uronic acids after derivatization with p-aminobenzoic acid.

Analytical chemistry

A Meyer, C Raba, K Fischer

Affiliations

  1. FB VI-Geography/Geosciences, Analytical and Ecological Chemistry, University of Trier, Germany.

PMID: 11403275 DOI: 10.1021/ac001402s

Abstract

A new, selective, and sensitive ion-pair RP-HPLC method for the simultaneous determination of three classes of natural organic compounds, i.e., carbohydrates, amino sugars, and uronic acids, in environmental samples is presented. p-Aminobenzoic acid is used for precolumn derivatization of the analytes, enabling fluorescence (lambda(ex) 313 nm, lambda(em) 358 nm) or photometric detection (303 nm). The dependence of the derivatization yield on the reaction conditions is examined. Derivatives of lactose, galactose, glucose, mannose, xylose, arabinose, galacturonic acid, glucuronic acid, N-acetylglucosamine, and glycerinealdehyde were separated on a RP-C18 column with hydrophilic end capping within 35 min, applying TBAHSO4 as the ion-pair reagent. The concentration detection limits range between 20 and 30 microg L(-1) ((1-2) x 10(-7) M) for fluorescence detection and between 30 and 75 microg L(-1) for UV detection. A good linearity is achieved in the concentration range from 50 microg L(-1) to 100 mg L(-1) (r2 > 0.99). The described method has been applied for the determination of mono-/disaccharides, uronic acids, and amino sugars in soil solutions and in landfill leachates.

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