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Moreno FA, McGavin C, Malan TP, et al. Tryptophan depletion selectively reduces CSF 5-HT metabolites in healthy young men: results from single lumbar puncture sampling technique. Int J Neuropsychopharmacol. 2000;3(4):277-283doi: 10.1017/S1461145700002133.
Moreno, F. A., McGavin, C., Malan, T. P., Gelenberg, A. J., Heninger, G. R., Mathé, A. A., & Delgado, P. L. (2000). Tryptophan depletion selectively reduces CSF 5-HT metabolites in healthy young men: results from single lumbar puncture sampling technique. The international journal of neuropsychopharmacology, 3(4), 277-283. https://doi.org/10.1017/S1461145700002133
Moreno, Francisco A., et al. "Tryptophan depletion selectively reduces CSF 5-HT metabolites in healthy young men: results from single lumbar puncture sampling technique." The international journal of neuropsychopharmacology vol. 3,4 (2000): 277-283. doi: https://doi.org/10.1017/S1461145700002133
Moreno FA, McGavin C, Malan TP, Gelenberg AJ, Heninger GR, Mathé AA, Delgado PL. Tryptophan depletion selectively reduces CSF 5-HT metabolites in healthy young men: results from single lumbar puncture sampling technique. Int J Neuropsychopharmacol. 2000 Dec;3(4):277-283. doi: 10.1017/S1461145700002133. PMID: 11343605.
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Int J Neuropsychopharmacol. 2000 Dec;3(4):277-283. doi: 10.1017/S1461145700002133.

Tryptophan depletion selectively reduces CSF 5-HT metabolites in healthy young men: results from single lumbar puncture sampling technique.

The international journal of neuropsychopharmacology

Francisco A. Moreno, Cameron McGavin, T. Phillip Malan, Alan J. Gelenberg, George R. Heninger, Aleksander A. Mathé, Pedro L. Delgado

PMID: 11343605 DOI: 10.1017/S1461145700002133

Abstract

Plasma tryptophan (Trp) depletion is a commonly used tool for determining the role of brain serotonin (5-HT) function in a variety of psychiatric disorders. This study measured the cerebrospinal fluid (CSF) monoamine metabolite response to Trp depletion and control testing in five healthy subjects utilizing a single lumbar puncture. Testing was done in a placebo-controlled, double-blind, randomized, cross-over design. Plasma-free and total Trp levels and behavioural ratings were obtained prior to and 5 h after ingestion of each amino-acid drink. CSF was obtained by performing a standard lumbar puncture 7 h after ingestion of the drink. Compared to control testing, Trp depletion caused a significant decrease of CSF 5-hydroxyindoleacetic acid (5-HIAA) (p = 0.03), but not of homovanillic acid or 3-methoxy-4-hydroxy-phenylglycol. Behavioural ratings were minimally affected in all subjects. This confirms that plasma Trp depletion reduces central nervous system measures of 5-HT function and suggests that the single lumbar puncture technique may be sufficient to detect the extent of CSF 5-HIAA changes during Trp depletion studies.

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