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Sahi SV, Chilton MD, Chilton WS. Corn metabolites affect growth and virulence of Agrobacterium tumefaciens. Proc Natl Acad Sci U S A. 1990;87(10):3879-83doi: 10.1073/pnas.87.10.3879.
Sahi, S. V., Chilton, M. D., & Chilton, W. S. (1990). Corn metabolites affect growth and virulence of Agrobacterium tumefaciens. Proceedings of the National Academy of Sciences of the United States of America, 87(10), 3879-83. https://doi.org/10.1073/pnas.87.10.3879
Sahi, S V, et al. "Corn metabolites affect growth and virulence of Agrobacterium tumefaciens." Proceedings of the National Academy of Sciences of the United States of America vol. 87,10 (1990): 3879-83. doi: https://doi.org/10.1073/pnas.87.10.3879
Sahi SV, Chilton MD, Chilton WS. Corn metabolites affect growth and virulence of Agrobacterium tumefaciens. Proc Natl Acad Sci U S A. 1990 May;87(10):3879-83. doi: 10.1073/pnas.87.10.3879. PMID: 11607078; PMCID: PMC54007.
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Proc Natl Acad Sci U S A. 1990 May;87(10):3879-83. doi: 10.1073/pnas.87.10.3879.

Corn metabolites affect growth and virulence of Agrobacterium tumefaciens.

Proceedings of the National Academy of Sciences of the United States of America

S V Sahi, M D Chilton, W S Chilton

Affiliations

  1. Department of Botany, North Carolina State University, Raleigh, NC 27695-7612, USA.

PMID: 11607078 PMCID: PMC54007 DOI: 10.1073/pnas.87.10.3879

Abstract

Homogenates of corn seedlings inhibit both growth of Agrobacterium tumefaciens and induction of its Ti plasmid virulence (vir) genes by acetosyringone (AS). The heat-labile inhibitor has been identified as 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (DIMBOA), present in 2-week-old seedlings (B73) at a concentration of 1.5 mM or greater. A concentration of 0.3 mM DIMBOA is sufficient to block growth of A. tumefaciens completely for 220 hr. DIMBOA at 0.1 mM concentration completely inhibited vir gene induction by 100 microM AS and reduced growth rate by 50%. Thus, DIMBOA can be expected to have a significant effect on attempts to transform corn by using A. tumefaciens as a vector.

References

  1. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4060-4 - PubMed
  2. Proc Natl Acad Sci U S A. 1986 Jan;83(2):379-83 - PubMed
  3. Proc Natl Acad Sci U S A. 1987 Aug;84(15):5345-9 - PubMed
  4. Plant Physiol. 1978 May;61(5):803-5 - PubMed
  5. Plant Physiol. 1978 May;61(5):796-802 - PubMed
  6. Plant Physiol. 1978 May;61(5):791-5 - PubMed
  7. Plant Physiol. 1979 Jan;63(1):14-9 - PubMed
  8. Science. 1989 Jun 16;244(4910):1293-9 - PubMed
  9. J Bacteriol. 1988 Sep;170(9):4047-54 - PubMed
  10. EMBO J. 1985 Apr;4(4):891-8 - PubMed
  11. EMBO J. 1986 Jul;5(7):1445-54 - PubMed
  12. Virology. 1988 Jan;162(1):248-50 - PubMed
  13. J Bacteriol. 1990 Feb;172(2):525-30 - PubMed
  14. J Bacteriol. 1990 Feb;172(2):1142-4 - PubMed
  15. Plasmid. 1978 Feb;1(2):238-53 - PubMed
  16. Biochemistry. 1967 Sep;6(9):2866-70 - PubMed

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