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Citovsky V, Knorr D, Zambryski P. Gene I, a potential cell-to-cell movement locus of cauliflower mosaic virus, encodes an RNA-binding protein. Proc Natl Acad Sci U S A. 1991;88(6):2476-80doi: 10.1073/pnas.88.6.2476.
Citovsky, V., Knorr, D., & Zambryski, P. (1991). Gene I, a potential cell-to-cell movement locus of cauliflower mosaic virus, encodes an RNA-binding protein. Proceedings of the National Academy of Sciences of the United States of America, 88(6), 2476-80. https://doi.org/10.1073/pnas.88.6.2476
Citovsky, V, et al. "Gene I, a potential cell-to-cell movement locus of cauliflower mosaic virus, encodes an RNA-binding protein." Proceedings of the National Academy of Sciences of the United States of America vol. 88,6 (1991): 2476-80. doi: https://doi.org/10.1073/pnas.88.6.2476
Citovsky V, Knorr D, Zambryski P. Gene I, a potential cell-to-cell movement locus of cauliflower mosaic virus, encodes an RNA-binding protein. Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2476-80. doi: 10.1073/pnas.88.6.2476. PMID: 11607169; PMCID: PMC51255.
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Proc Natl Acad Sci U S A. 1991 Mar 15;88(6):2476-80. doi: 10.1073/pnas.88.6.2476.

Gene I, a potential cell-to-cell movement locus of cauliflower mosaic virus, encodes an RNA-binding protein.

Proceedings of the National Academy of Sciences of the United States of America

V Citovsky, D Knorr, P Zambryski

Affiliations

  1. Department of Plant Biology, University of California, Berkeley, CA 94720, USA.

PMID: 11607169 PMCID: PMC51255 DOI: 10.1073/pnas.88.6.2476

Abstract

Cauliflower mosaic virus (CaMV) is a double-stranded DNA (dsDNA) pararetrovirus capable of cell-to-cell movement presumably through intercellular connections, the plasmodesmata, of the infected plant. This movement is likely mediated by a specific viral protein encoded by the gene I locus. Here we report that the purified gene I protein binds RNA and single-stranded DNA (ssDNA) but not dsDNA regardless of nucleotide sequence specificity. The binding is highly cooperative, and the affinity of the gene I protein for RNA is 10-fold higher than for ssDNA. CaMV replicates by reverse transcription of a 358 RNA that is homologous to the entire genome. We propose that the 35S RNA may be involved in cell-to-cell movement of CaMV as an intermediate that is transported through plasmodesmata as an RNA-gene I protein complex.

References

  1. EMBO J. 1986 Dec 1;5(12):3083-90 - PubMed
  2. EMBO J. 1984 Dec 1;3(12):2731-6 - PubMed
  3. Science. 1988 Apr 22;240(4851):501-4 - PubMed
  4. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
  5. Nucleic Acids Res. 1990 Sep 25;18(18):5505-13 - PubMed
  6. Adv Virus Res. 1990;38:201-48 - PubMed
  7. Proc Natl Acad Sci U S A. 1985 Aug;82(16):5260-4 - PubMed
  8. Mol Biol Evol. 1985 Nov;2(6):455-68 - PubMed
  9. Gene. 1987;56(1):125-35 - PubMed
  10. Adv Virus Res. 1987;32:35-96 - PubMed
  11. J Mol Biol. 1986 May 5;189(1):113-30 - PubMed
  12. Annu Rev Biochem. 1986;55:103-36 - PubMed
  13. Virology. 1989 Oct;172(2):451-9 - PubMed
  14. Bioessays. 1989 Jun;10(6):179-86 - PubMed
  15. Cell. 1989 Dec 22;59(6):1135-43 - PubMed
  16. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9203-7 - PubMed
  17. Virology. 1989 Apr;169(2):436-46 - PubMed
  18. Proc Natl Acad Sci U S A. 1989 Feb;86(4):1193-7 - PubMed
  19. Virology. 1988 Apr;163(2):503-8 - PubMed
  20. Adv Virus Res. 1984;29:313-64 - PubMed
  21. J Mol Biol. 1986 Feb 20;187(4):603-15 - PubMed
  22. Cell. 1987 Dec 24;51(6):1145-57 - PubMed
  23. Virology. 1987 Jun;158(2):444-6 - PubMed
  24. Cell. 1990 Feb 23;60(4):637-47 - PubMed
  25. J Virol. 1990 Jun;64(6):2594-8 - PubMed
  26. J Gen Virol. 1990 May;71 ( Pt 5):1009-18 - PubMed
  27. Virology. 1990 Feb;174(2):622-4 - PubMed
  28. Methods Enzymol. 1983;101:20-78 - PubMed
  29. Nucleic Acids Res. 1981 Jun 25;9(12):2871-88 - PubMed
  30. Nucleic Acids Res. 1983 Dec 20;11(24):8901-14 - PubMed
  31. Proc Natl Acad Sci U S A. 1982 Oct;79(19):5818-22 - PubMed
  32. Cell. 1982 Oct;30(3):763-73 - PubMed
  33. Cell. 1982 Jun;29(2):395-402 - PubMed
  34. Cell. 1980 Aug;21(1):285-94 - PubMed
  35. Eur J Biochem. 1977 Dec;81(3):557-62 - PubMed
  36. Nucleic Acids Res. 1978 Aug;5(8):2913-25 - PubMed
  37. J Ultrastruct Res. 1976 Sep;56(3):351-64 - PubMed
  38. Virology. 1969 Apr;37(4):681-5 - PubMed
  39. Gene. 1985;33(1):103-19 - PubMed

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