Display options
Cite
Tinland B, Hohn B, Puchta H. Agrobacterium tumefaciens transfers single-stranded transferred DNA (T-DNA) into the plant cell nucleus. Proc Natl Acad Sci U S A. 1994;91(17):8000-4doi: 10.1073/pnas.91.17.8000.
Tinland, B., Hohn, B., & Puchta, H. (1994). Agrobacterium tumefaciens transfers single-stranded transferred DNA (T-DNA) into the plant cell nucleus. Proceedings of the National Academy of Sciences of the United States of America, 91(17), 8000-4. https://doi.org/10.1073/pnas.91.17.8000
Tinland, B, et al. "Agrobacterium tumefaciens transfers single-stranded transferred DNA (T-DNA) into the plant cell nucleus." Proceedings of the National Academy of Sciences of the United States of America vol. 91,17 (1994): 8000-4. doi: https://doi.org/10.1073/pnas.91.17.8000
Tinland B, Hohn B, Puchta H. Agrobacterium tumefaciens transfers single-stranded transferred DNA (T-DNA) into the plant cell nucleus. Proc Natl Acad Sci U S A. 1994 Aug 16;91(17):8000-4. doi: 10.1073/pnas.91.17.8000. PMID: 11607492; PMCID: PMC44532.
Copy Download .nbib Format: NLM
Share it on

Proc Natl Acad Sci U S A. 1994 Aug 16;91(17):8000-4. doi: 10.1073/pnas.91.17.8000.

Agrobacterium tumefaciens transfers single-stranded transferred DNA (T-DNA) into the plant cell nucleus.

Proceedings of the National Academy of Sciences of the United States of America

B Tinland, B Hohn, H Puchta

Affiliations

  1. Friedrich Miescher-Institute, Basel, Switzerland.

PMID: 11607492 PMCID: PMC44532 DOI: 10.1073/pnas.91.17.8000

Abstract

Transferred DNA (T-DNA) is transferred as a single-stranded derivative from Agrobacterium to the plant cell nucleus. This conclusion is drawn from experiments exploiting the different properties of single- and double-stranded DNA to perform extrachromosomal homologous recombination in plant cells. After transfer from Agrobacterium to plant cells, T-DNA molecules recombined much more efficiently if the homologous sequences were of opposite polarity than if they were of the same polarity. This observation reflects the properties of single-stranded DNA; single-stranded DNA molecules of opposite polarity can anneal directly, whereas single-stranded DNA molecules of the same polarity first have to become double stranded to anneal. Judging from the relative amounts of single- to double-stranded T-DNA derivatives undergoing recombination, we infer that the T-DNA derivatives enter the plant nucleus in their single-stranded form.

References

  1. Nucleic Acids Res. 1991 May 25;19(10):2693-700 - PubMed
  2. Mol Gen Genet. 1991 Nov;230(1-2):1-7 - PubMed
  3. Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1456-60 - PubMed
  4. Genes Dev. 1991 Feb;5(2):287-97 - PubMed
  5. Proc Natl Acad Sci U S A. 1990 Dec;87(24):9534-7 - PubMed
  6. Plant Mol Biol. 1990 Jan;14(1):61-72 - PubMed
  7. EMBO J. 1990 Oct;9(10):3077-84 - PubMed
  8. J Bacteriol. 1988 Aug;170(8):3367-74 - PubMed
  9. J Bacteriol. 1989 Oct;171(10):5281-9 - PubMed
  10. Mol Gen Genet. 1989 Apr;216(2-3):428-38 - PubMed
  11. J Bacteriol. 1987 Mar;169(3):1046-55 - PubMed
  12. Mol Cell Biol. 1985 Jan;5(1):59-69 - PubMed
  13. Annu Rev Genet. 1988;22:1-30 - PubMed
  14. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9154-8 - PubMed
  15. Nucleic Acids Res. 1989 Aug 25;17(16):6747 - PubMed
  16. Cell. 1989 Jan 27;56(2):193-201 - PubMed
  17. Cell. 1989 Jun 2;57(5):847-57 - PubMed
  18. Proc Natl Acad Sci U S A. 1989 Apr;86(7):2133-7 - PubMed
  19. J Mol Biol. 1988 Sep 20;203(2):373-84 - PubMed
  20. Cell. 1986 Oct 24;47(2):155-7 - PubMed
  21. EMBO J. 1990 Apr;9(4):1177-85 - PubMed
  22. Mol Cell Biol. 1984 Jun;4(6):1020-34 - PubMed
  23. Cell. 1977 Jun;11(2):263-71 - PubMed
  24. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4060-4 - PubMed
  25. Proc Natl Acad Sci U S A. 1987 Apr;84(7):1881-5 - PubMed
  26. Proc Natl Acad Sci U S A. 1989 Jun;86(11):4017-21 - PubMed
  27. Science. 1907 Apr 26;25(643):671-3 - PubMed
  28. EMBO J. 1986 Jun;5(6):1129-35 - PubMed
  29. Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):2994-8 - PubMed
  30. Proc Natl Acad Sci U S A. 1994 Jan 18;91(2):694-8 - PubMed
  31. Plant Mol Biol. 1989 Dec;13(6):711-9 - PubMed
  32. Mol Gen Genet. 1993 Jun;239(3):345-53 - PubMed
  33. Science. 1992 Jun 26;256(5065):1802-5 - PubMed
  34. Cell. 1992 Jan 10;68(1):109-18 - PubMed
  35. Proc Natl Acad Sci U S A. 1993 Dec 15;90(24):11538-42 - PubMed
  36. Nucleic Acids Res. 1993 Nov 11;21(22):5034-40 - PubMed
  37. Nucleic Acids Res. 1992 Jun 11;20(11):2785-94 - PubMed
  38. Plant Mol Biol. 1990 Feb;14(2):269-76 - PubMed
  39. Mol Microbiol. 1990 Jul;4(7):1199-205 - PubMed
  40. J Bacteriol. 1992 Jan;174(1):303-8 - PubMed
  41. Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):11837-41 - PubMed
  42. Plant Mol Biol. 1992 Sep;19(6):1019-30 - PubMed
  43. Proc Natl Acad Sci U S A. 1992 Aug 15;89(16):7442-6 - PubMed
  44. Mol Cell Biol. 1992 Aug;12(8):3372-9 - PubMed
  45. Plant Mol Biol. 1992 May;19(1):15-38 - PubMed
  46. Mol Cell Biol. 1992 Jan;12(1):329-36 - PubMed
  47. Nucleic Acids Res. 1991 Jun 25;19(12):3455 - PubMed
  48. Mol Gen Genet. 1991 Nov;230(1-2):209-18 - PubMed
  49. EMBO J. 1991 Mar;10(3):697-704 - PubMed

Publication Types