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Karlsson G. Thickness measurements of lacey carbon films. J Microsc. 2001;203:326-8doi: 10.1046/j.1365-2818.2001.00930.x.
Karlsson, G. (2001). Thickness measurements of lacey carbon films. Journal of microscopy, 203326-8. https://doi.org/10.1046/j.1365-2818.2001.00930.x
Karlsson, G. "Thickness measurements of lacey carbon films." Journal of microscopy vol. 203 (2001): 326-8. doi: https://doi.org/10.1046/j.1365-2818.2001.00930.x
Karlsson G. Thickness measurements of lacey carbon films. J Microsc. 2001 Sep;203:326-8. doi: 10.1046/j.1365-2818.2001.00930.x. PMID: 11555151.
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J Microsc. 2001 Sep;203:326-8. doi: 10.1046/j.1365-2818.2001.00930.x.

Thickness measurements of lacey carbon films.

Journal of microscopy

G Karlsson

Affiliations

  1. The Biomicroscopy Unit, Inorganic Chemistry 2, Chemical Centre, PO Box 124, Lund University, S 221 00 Lund, Sweden. [email protected]

PMID: 11555151 DOI: 10.1046/j.1365-2818.2001.00930.x

Abstract

Lacey carbon filmed grids interact differently with liquid samples during plunging and subsequent studies in cryo-TEM depending on type and manufacture. One important parameter is the thickness of the film, which is investigated here by ultrathin sectioning. The film thickness of the carbon-Formvar type is found to be 100-200 nm, with the variation of the thickness occurring in the polymer layer. The pure carbon type is found to be 20-30 nm thick. Knowing the thickness makes it easier to choose the optimal type for each cryo-TEM sample.

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