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Satoh MI, Hovington H, Cadrin M. Reduction of cytochemical ecto-ATPase activities in keratin 8-deficient FVB/N mouse livers. Med Electron Microsc. 1999;32(4):209-212doi: 10.1007/s007959900003.
Satoh, M. I., Hovington, H., & Cadrin, M. (1999). Reduction of cytochemical ecto-ATPase activities in keratin 8-deficient FVB/N mouse livers. Medical electron microscopy : official journal of the Clinical Electron Microscopy Society of Japan, 32(4), 209-212. https://doi.org/10.1007/s007959900003
Satoh, M.I., et al. "Reduction of cytochemical ecto-ATPase activities in keratin 8-deficient FVB/N mouse livers." Medical electron microscopy : official journal of the Clinical Electron Microscopy Society of Japan vol. 32,4 (1999): 209-212. doi: https://doi.org/10.1007/s007959900003
Satoh MI, Hovington H, Cadrin M. Reduction of cytochemical ecto-ATPase activities in keratin 8-deficient FVB/N mouse livers. Med Electron Microsc. 1999 Dec;32(4):209-212. doi: 10.1007/s007959900003. PMID: 11810447.
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Med Electron Microsc. 1999 Dec;32(4):209-212. doi: 10.1007/s007959900003.

Reduction of cytochemical ecto-ATPase activities in keratin 8-deficient FVB/N mouse livers.

Medical electron microscopy : official journal of the Clinical Electron Microscopy Society of Japan

M.I. Satoh, Hélène Hovington, M. Cadrin

Affiliations

  1. Département de chimie-biologie, Université du Québec à Trois-Rivières, C.P.500 Trois-Rivières, Québec, Canada G9A 5H7. [email protected]

PMID: 11810447 DOI: 10.1007/s007959900003

Abstract

Keratin 8 (K8) and keratin 18 are the intermediate filament proteins that are expressed in hepatocytes. A K8-deficient FVB/N mouse is a unique animal model for assessing the contribution of keratin intermediate filaments (IFs) to the structural and functional integrity of hepatocytes. Hepatocytes from homozygous (-/-) K8-deficient mice manifest a reduced bile acid secretion and an increased fragility to mechanical stress and hepatotoxic drugs. Hepatocytes from heterozygous (+/-) mice are more susceptible to drug-induced injury. Immunofluorescent microscopy revealed that hepatocytes from (+/-) mice maintained K8 IFs and F-actin that are similar to those in wild-type (+/+) mouse hepatocytes. In (-/-) mouse hepatocytes, K8 protein was negative and F-actin presented a coarse and irregular pattern. Ecto-ATPase, detected by enzyme histochemistry and observed by electron microscopy, was reduced in the bile canaliculi of both (+/-) and (-/-) mouse livers, in comparison with that of (+/+) mouse livers. These results reveal for the first time different microscopical findings regarding the livers of these three genotypes. They also suggest that the reduction of ecto-ATPase plays a role in the increased fragility of (+/-) and (-/-) mouse livers.

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